AAEB_ECOLI
ID AAEB_ECOLI Reviewed; 655 AA.
AC P46481; Q2M8X3;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=p-hydroxybenzoic acid efflux pump subunit AaeB {ECO:0000255|HAMAP-Rule:MF_01545};
DE Short=pHBA efflux pump protein B {ECO:0000255|HAMAP-Rule:MF_01545};
GN Name=aaeB {ECO:0000255|HAMAP-Rule:MF_01545}; Synonyms=yhcP;
GN OrderedLocusNames=b3240, JW3209;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION, AND INDUCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15489430; DOI=10.1128/jb.186.21.7196-7204.2004;
RA Van Dyk T.K., Templeton L.J., Cantera K.A., Sharpe P.L., Sariaslani F.S.;
RT "Characterization of the Escherichia coli AaeAB efflux pump: a metabolic
RT relief valve?";
RL J. Bacteriol. 186:7196-7204(2004).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Forms an efflux pump with AaeA. Could function as a metabolic
CC relief valve, allowing to eliminate certain compounds when they
CC accumulate to high levels in the cell. Substrates are p-hydroxybenzoic
CC acid (pHBA), 6-hydroxy-2-naphthoic and 2-hydroxycinnamate.
CC {ECO:0000255|HAMAP-Rule:MF_01545, ECO:0000269|PubMed:15489430}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- INDUCTION: Positively coregulated with aaeA and aaeX by AaeR.
CC {ECO:0000255|HAMAP-Rule:MF_01545, ECO:0000269|PubMed:15489430}.
CC -!- SIMILARITY: Belongs to the aromatic acid exporter ArAE (TC 2.A.85)
CC family. {ECO:0000255|HAMAP-Rule:MF_01545}.
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DR EMBL; U18997; AAA58042.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76272.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77283.1; -; Genomic_DNA.
DR PIR; B65116; B65116.
DR RefSeq; NP_417707.1; NC_000913.3.
DR RefSeq; WP_000510991.1; NZ_CP064683.1.
DR AlphaFoldDB; P46481; -.
DR BioGRID; 4263393; 383.
DR IntAct; P46481; 2.
DR STRING; 511145.b3240; -.
DR TCDB; 2.A.85.1.2; the aromatic acid exporter (arae) family.
DR PaxDb; P46481; -.
DR PRIDE; P46481; -.
DR EnsemblBacteria; AAC76272; AAC76272; b3240.
DR EnsemblBacteria; BAE77283; BAE77283; BAE77283.
DR GeneID; 947747; -.
DR KEGG; ecj:JW3209; -.
DR KEGG; eco:b3240; -.
DR PATRIC; fig|511145.12.peg.3337; -.
DR EchoBASE; EB2673; -.
DR eggNOG; COG1289; Bacteria.
DR HOGENOM; CLU_027647_0_0_6; -.
DR InParanoid; P46481; -.
DR PhylomeDB; P46481; -.
DR BioCyc; EcoCyc:G7685-MON; -.
DR BioCyc; MetaCyc:G7685-MON; -.
DR PRO; PR:P46481; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046942; P:carboxylic acid transport; IMP:EcoliWiki.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR HAMAP; MF_01545; AaeB; 1.
DR InterPro; IPR006726; PHBA_efflux_AaeB/fusaric-R.
DR InterPro; IPR023706; PHBA_efflux_pump_AaeB.
DR PANTHER; PTHR30509:SF10; PTHR30509:SF10; 1.
DR Pfam; PF04632; FUSC; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..655
FT /note="p-hydroxybenzoic acid efflux pump subunit AaeB"
FT /id="PRO_0000210077"
FT TOPO_DOM 1..12
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01545"
FT TOPO_DOM 34..37
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01545"
FT TOPO_DOM 59..68
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01545"
FT TOPO_DOM 90..92
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01545"
FT TOPO_DOM 114..120
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01545"
FT TOPO_DOM 142..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01545"
FT TOPO_DOM 173..369
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 370..390
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01545"
FT TOPO_DOM 391..406
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01545"
FT TOPO_DOM 428..430
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 431..451
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01545"
FT TOPO_DOM 452..458
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 459..479
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01545"
FT TOPO_DOM 480..481
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01545"
FT TOPO_DOM 503..655
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 655 AA; 73591 MW; 6C559FC19C276656 CRC64;
MGIFSIANQH IRFAVKLATA IVLALFVGFH FQLETPRWAV LTAAIVAAGT AFAAGGEPYS
GAIRYRGFLR IIGTFIGCIA GLVIIIAMIR APLLMILVCC IWAGFCTWIS SLVRIENSYA
WGLAGYTALI IVITIQPEPL LTPQFAVERC SEIVIGIVCA IMADLLFSPR SIKQEVDREL
ESLLVAQYQL MQLCIKHGDG EVVDKAWGDL VRRTTALQGM RSNLNMESSR WARANRRLKA
INTLSLTLIT QSCETYLIQN TRPELITDTF REFFDTPVET AQDVHKQLKR LRRVIAWTGE
RETPVTIYSW VAAATRYQLL KRGVISNTKI NATEEEILQG EPEVKVESAE RHHAMVNFWR
TTLSCILGTL FWLWTGWTSG SGAMVMIAVV TSLAMRLPNP RMVAIDFIYG TLAALPLGLL
YFLVIIPNTQ QSMLLLCISL AVLGFFLGIE VQKRRLGSMG ALASTINIIV LDNPMTFHFS
QFLDSALGQI VGCVLAFTVI LLVRDKSRDR TGRVLLNQFV SAAVSAMTTN VARRKENHLP
ALYQQLFLLM NKFPGDLPKF RLALTMIIAH QRLRDAPIPV NEDLSAFHRQ MRRTADHVIS
ARSDDKRRRY FGQLLEELEI YQEKLRIWQA PPQVTEPVNR LAGMLHKYQH ALTDS
