QPT1B_TOBAC
ID QPT1B_TOBAC Reviewed; 350 AA.
AC A0A1S4DF18; A0A1S4DFD0;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 1.
DT 03-AUG-2022, entry version 22.
DE RecName: Full=Quinolinate phosphoribosyltransferase [decarboxylating] 1b {ECO:0000305};
DE Short=NtQPT1b {ECO:0000305};
DE EC=2.4.2.19 {ECO:0000305|PubMed:23953973, ECO:0000305|PubMed:25582664};
GN Name=QPT1b {ECO:0000305};
GN ORFNames=LOC107829123 {ECO:0000312|RefSeq:XP_016512047.1};
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TN90;
RX PubMed=24807620; DOI=10.1038/ncomms4833;
RA Sierro N., Battey J.N., Ouadi S., Bakaher N., Bovet L., Willig A.,
RA Goepfert S., Peitsch M.C., Ivanov N.V.;
RT "The tobacco genome sequence and its comparison with those of tomato and
RT potato.";
RL Nat. Commun. 5:3833-3833(2014).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND REVIEW ON ALKALOID BIOSYNTHESIS
RP IN NICOTIANA TABACUM.
RX PubMed=23953973; DOI=10.1016/j.phytochem.2013.06.002;
RA Dewey R.E., Xie J.;
RT "Molecular genetics of alkaloid biosynthesis in Nicotiana tabacum.";
RL Phytochemistry 94:10-27(2013).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND REVIEW ON NICOTINE BIOSYNTHESIS.
RX PubMed=25582664; DOI=10.1007/s00438-015-0989-7;
RA Wang X., Bennetzen J.L.;
RT "Current status and prospects for the study of Nicotiana genomics,
RT genetics, and nicotine biosynthesis genes.";
RL Mol. Genet. Genomics 290:11-21(2015).
RN [4]
RP FUNCTION.
RC STRAIN=cv. Burley Stella, cv. Burley TN90, cv. Virginia ITB 683, and
RC cv. Virginia K326;
RX PubMed=31276744; DOI=10.1016/j.fct.2019.110660;
RA Schorderet Weber S., Kaminski K.P., Perret J.-L., Leroy P., Mazurov A.,
RA Peitsch M.C., Ivanov N.V., Hoeng J.;
RT "Antiparasitic properties of leaf extracts derived from selected Nicotiana
RT species and Nicotiana tabacum varieties.";
RL Food Chem. Toxicol. 132:110660-110660(2019).
CC -!- FUNCTION: Involved in the biosynthesis of pyridine alkaloid natural
CC products, leading mainly to the production of anabasine, anatabine,
CC nicotine and nornicotine, effective deterrents against herbivores with
CC antiparasitic and pesticide properties (neurotoxins); nornicotine
CC serves as the precursor in the synthesis of the carcinogen compound N'-
CC nitrosonornicotine (NNN) (Probable) (PubMed:31276744). Involved in the
CC catabolism of quinolinic acid (QA) (Probable).
CC {ECO:0000269|PubMed:31276744, ECO:0000305|PubMed:23953973,
CC ECO:0000305|PubMed:25582664}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=CO2 + diphosphate + nicotinate beta-D-ribonucleotide = 5-
CC phospho-alpha-D-ribose 1-diphosphate + 2 H(+) + quinolinate;
CC Xref=Rhea:RHEA:12733, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:29959, ChEBI:CHEBI:33019, ChEBI:CHEBI:57502,
CC ChEBI:CHEBI:58017; EC=2.4.2.19;
CC Evidence={ECO:0000305|PubMed:23953973, ECO:0000305|PubMed:25582664};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:12735;
CC Evidence={ECO:0000305|PubMed:23953973, ECO:0000305|PubMed:25582664};
CC -!- PATHWAY: Alkaloid biosynthesis; nicotine biosynthesis.
CC {ECO:0000305|PubMed:23953973, ECO:0000305|PubMed:25582664}.
CC -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-
CC ribonucleotide from quinolinate: step 1/1.
CC {ECO:0000305|PubMed:23953973, ECO:0000305|PubMed:25582664}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A0A1S4DF18-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A0A1S4DF18-2; Sequence=VSP_061528;
CC -!- SIMILARITY: Belongs to the NadC/ModD family. {ECO:0000305}.
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DR RefSeq; XP_016512047.1; XM_016656561.1.
DR RefSeq; XP_016512048.1; XM_016656562.1.
DR SMR; A0A1S4DF18; -.
DR GeneID; 107829123; -.
DR KEGG; nta:107829123; -.
DR OMA; CPRKAIP; -.
DR OrthoDB; 1263431at2759; -.
DR UniPathway; UPA00107; -.
DR UniPathway; UPA00253; UER00331.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004514; F:nicotinate-nucleotide diphosphorylase (carboxylating) activity; IBA:GO_Central.
DR GO; GO:0009435; P:NAD biosynthetic process; IBA:GO_Central.
DR GO; GO:0034213; P:quinolinate catabolic process; IBA:GO_Central.
DR CDD; cd01572; QPRTase; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR Gene3D; 3.90.1170.20; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR004393; NadC.
DR InterPro; IPR027277; NadC/ModD.
DR InterPro; IPR036068; Nicotinate_pribotase-like_C.
DR InterPro; IPR037128; Quinolinate_PRibosylTase_N_sf.
DR InterPro; IPR002638; Quinolinate_PRibosylTrfase_C.
DR InterPro; IPR022412; Quinolinate_PRibosylTrfase_N.
DR PANTHER; PTHR32179; PTHR32179; 1.
DR Pfam; PF01729; QRPTase_C; 1.
DR Pfam; PF02749; QRPTase_N; 1.
DR PIRSF; PIRSF006250; NadC_ModD; 1.
DR SUPFAM; SSF51690; SSF51690; 1.
DR TIGRFAMs; TIGR00078; nadC; 1.
PE 1: Evidence at protein level;
KW Alkaloid metabolism; Alternative splicing; Glycosyltransferase;
KW Pyridine nucleotide biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..350
FT /note="Quinolinate phosphoribosyltransferase
FT [decarboxylating] 1b"
FT /id="PRO_0000455794"
FT BINDING 141
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WJJ7"
FT BINDING 172..174
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WJJ7"
FT BINDING 196
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WJJ7"
FT BINDING 206
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WJJ7"
FT BINDING 239
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WJJ7"
FT BINDING 266
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WJJ7"
FT BINDING 298..300
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WJJ7"
FT BINDING 319..321
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P9WJJ7"
FT VAR_SEQ 1..24
FT /note="Missing (in isoform 2)"
FT /id="VSP_061528"
SQ SEQUENCE 350 AA; 37819 MW; 98EB6816B54AB3F9 CRC64;
MFKVLPFTAI VHPNAITAPR LVVKMSAIAT KNAVESFVVK PPAHPTYDLK GVIQLALSED
AGDIGDVTCK ATIPIDMESE AHFLAKEDGI VAGIALAEMI FAEVDPSLKM EWSINDGDKV
HKGLKFGKVQ GKAHSIVIAE RVVLNFMQRM SGIATLTKAM ADAAHPATIL ETRKTAPGLR
LVDKWAVLIG GGKNHRMGLF DMVMIKDNHI SAAGGVSKAL NSVDQYLEQN KLQMGVEVET
RTIAEVHEVL EYASQTKTSL TRIMLDNMVV PLSNGDIEVS MLEEAVDLIN GRFETEASGN
VTLETVHKIG QTGVTYISSG ALTHSVKALD ISLKIDTELA LEVGRRTKRA
