QPTR_ECOLI
ID QPTR_ECOLI Reviewed; 221 AA.
AC P37619; Q2M7D4;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Queuosine precursor transporter {ECO:0000305};
DE Short=Q precursor transporter {ECO:0000255|HAMAP-Rule:MF_02088, ECO:0000305};
GN Name=yhhQ; OrderedLocusNames=b3471, JW3436;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=28208705; DOI=10.3390/biom7010012;
RA Zallot R., Yuan Y., de Crecy-Lagard V.;
RT "The Escherichia coli COG1738 member YhhQ is involved in 7-
RT cyanodeazaguanine (preQ(0)) transport.";
RL Biomolecules 7:0-0(2017).
CC -!- FUNCTION: Involved in the import of queuosine (Q) precursors, required
CC for Q precursor salvage. Transports 7-cyano-7-deazaguanine (preQ(0))
CC and 7-aminomethyl-7-deazaguanine (preQ(1)), with a preference for
CC preQ(0). {ECO:0000269|PubMed:28208705}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_02088, ECO:0000269|PubMed:15919996}; Multi-pass membrane
CC protein {ECO:0000255|HAMAP-Rule:MF_02088}.
CC -!- DISRUPTION PHENOTYPE: YhhQ-queD double mutant cannot produce Q modified
CC tRNA in minimal media with preQ(0) or preQ(1). YhhQ expressed from a
CC plasmid restores the presence of Q modified tRNA.
CC {ECO:0000269|PubMed:28208705}.
CC -!- SIMILARITY: Belongs to the vitamin uptake transporter (VUT/ECF) (TC
CC 2.A.88) family. Q precursor transporter subfamily. {ECO:0000255|HAMAP-
CC Rule:MF_02088, ECO:0000305}.
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DR EMBL; U00039; AAB18446.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76496.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77822.1; -; Genomic_DNA.
DR PIR; S47690; S47690.
DR RefSeq; NP_417928.1; NC_000913.3.
DR RefSeq; WP_001100469.1; NZ_SSZK01000008.1.
DR AlphaFoldDB; P37619; -.
DR STRING; 511145.b3471; -.
DR PaxDb; P37619; -.
DR PRIDE; P37619; -.
DR EnsemblBacteria; AAC76496; AAC76496; b3471.
DR EnsemblBacteria; BAE77822; BAE77822; BAE77822.
DR GeneID; 947984; -.
DR KEGG; ecj:JW3436; -.
DR KEGG; eco:b3471; -.
DR PATRIC; fig|1411691.4.peg.3254; -.
DR EchoBASE; EB2131; -.
DR eggNOG; COG1738; Bacteria.
DR HOGENOM; CLU_090905_0_0_6; -.
DR InParanoid; P37619; -.
DR OMA; IWLSLFH; -.
DR PhylomeDB; P37619; -.
DR BioCyc; EcoCyc:EG12217-MON; -.
DR BioCyc; MetaCyc:EG12217-MON; -.
DR PRO; PR:P37619; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0072531; P:pyrimidine-containing compound transmembrane transport; IDA:EcoCyc.
DR GO; GO:1990397; P:queuosine salvage; IDA:EcoCyc.
DR HAMAP; MF_02088; Q_prec_transport; 1.
DR InterPro; IPR003744; YhhQ.
DR PANTHER; PTHR34300; PTHR34300; 1.
DR Pfam; PF02592; Vut_1; 1.
DR TIGRFAMs; TIGR00697; TIGR00697; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..221
FT /note="Queuosine precursor transporter"
FT /id="PRO_0000169556"
FT TOPO_DOM 1..12
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02088"
FT TOPO_DOM 34
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02088"
FT TOPO_DOM 56..70
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02088"
FT TOPO_DOM 92..97
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02088"
FT TOPO_DOM 119..143
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02088"
FT TOPO_DOM 165..184
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02088"
FT TOPO_DOM 206..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:15919996"
SQ SEQUENCE 221 AA; 25269 MW; E3D90237BEE7ABCC CRC64;
MNVFSQTQRY KALFWLSLFH LLVITSSNYL VQLPVSILGF HTTWGAFSFP FIFLATDLTV
RIFGAPLARR IIFAVMIPAL LISYVISSLF YMGSWQGFGA LAHFNLFVAR IATASFMAYA
LGQILDVHVF NRLRQSRRWW LAPTASTLFG NVSDTLAFFF IAFWRSPDAF MAEHWMEIAL
VDYCFKVLIS IVFFLPMYGV LLNMLLKRLA DKSEINALQA S