QQT1_ARATH
ID QQT1_ARATH Reviewed; 298 AA.
AC Q56XY2; Q9FMR4;
DT 28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=GPN-loop GTPase QQT1 {ECO:0000305};
DE EC=3.6.5.- {ECO:0000305};
DE AltName: Full=Protein EMBRYO DEFECTIVE 1705 {ECO:0000303|PubMed:17419841};
DE AltName: Full=Protein QUATRE QUART 1 {ECO:0000303|PubMed:17419841};
GN Name=QQT1 {ECO:0000303|PubMed:17419841};
GN Synonyms=EMB1705 {ECO:0000303|PubMed:17419841};
GN OrderedLocusNames=At5g22370 {ECO:0000312|Araport:AT5G22370};
GN ORFNames=MWD9.17 {ECO:0000312|EMBL:BAB08336.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, INTERACTION WITH QQT2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=17419841; DOI=10.1111/j.1365-313x.2007.03072.x;
RA Lahmy S., Guilleminot J., Schmit A.C., Pelletier G., Chaboute M.E.,
RA Devic M.;
RT "QQT proteins colocalize with microtubules and are essential for early
RT embryo development in Arabidopsis.";
RL Plant J. 50:615-626(2007).
RN [5]
RP FUNCTION.
RX PubMed=28475733; DOI=10.1093/jxb/erx138;
RA Kwak K.J., Kim B.M., Lee K., Kang H.;
RT "quatre-quart1 is an indispensable U12 intron-containing gene that plays a
RT crucial role in Arabidopsis development.";
RL J. Exp. Bot. 68:2731-2739(2017).
CC -!- FUNCTION: Small GTPase that is essential for the correct formation of
CC the tangential divisions in early embryos. Associates with microtubule
CC during mitosis and may function in the positioning of the division
CC plane. May participate in the patterning of the early embryo at the
CC octant-dermatogen transition (PubMed:17419841). Is crucial for normal
CC development of the plant (PubMed:28475733).
CC {ECO:0000269|PubMed:17419841, ECO:0000269|PubMed:28475733}.
CC -!- SUBUNIT: Heterodimer with QQT2. {ECO:0000269|PubMed:17419841}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17419841}. Nucleus
CC {ECO:0000269|PubMed:17419841}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:17419841}. Cytoplasm, cytoskeleton, phragmoplast
CC {ECO:0000269|PubMed:17419841}. Note=During interphase, mainly expressed
CC in the cytoplasm and weakly in the nucleus. Associated with
CC microtubules during cell division. {ECO:0000269|PubMed:17419841}.
CC -!- TISSUE SPECIFICITY: Expressed in vascular tissues, root tips, apical
CC and root meristematic regions, and floral primordia.
CC {ECO:0000269|PubMed:17419841}.
CC -!- DEVELOPMENTAL STAGE: During embryogenesis, expressed in the embryo from
CC octant to torpedo stage. {ECO:0000269|PubMed:17419841}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality due to embryo development
CC arrest at the octant stage. {ECO:0000269|PubMed:17419841}.
CC -!- MISCELLANEOUS: Plants silencing QQT1 exhibit severe defects in growth
CC and development, such as severely arrested stem growth, small size,
CC formation of serrated leaves and abnormal structures of the shoot
CC apical meristems. {ECO:0000269|PubMed:28475733}.
CC -!- SIMILARITY: Belongs to the GPN-loop GTPase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08336.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB007651; BAB08336.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED93018.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93019.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70766.1; -; Genomic_DNA.
DR EMBL; AK221541; BAD94895.1; -; mRNA.
DR RefSeq; NP_001119261.1; NM_001125789.1.
DR RefSeq; NP_001332350.1; NM_001343725.1.
DR RefSeq; NP_197629.2; NM_122142.4.
DR AlphaFoldDB; Q56XY2; -.
DR SMR; Q56XY2; -.
DR IntAct; Q56XY2; 1.
DR STRING; 3702.AT5G22370.1; -.
DR iPTMnet; Q56XY2; -.
DR PaxDb; Q56XY2; -.
DR PRIDE; Q56XY2; -.
DR ProteomicsDB; 226029; -.
DR EnsemblPlants; AT5G22370.1; AT5G22370.1; AT5G22370.
DR EnsemblPlants; AT5G22370.2; AT5G22370.2; AT5G22370.
DR EnsemblPlants; AT5G22370.3; AT5G22370.3; AT5G22370.
DR GeneID; 832298; -.
DR Gramene; AT5G22370.1; AT5G22370.1; AT5G22370.
DR Gramene; AT5G22370.2; AT5G22370.2; AT5G22370.
DR Gramene; AT5G22370.3; AT5G22370.3; AT5G22370.
DR KEGG; ath:AT5G22370; -.
DR Araport; AT5G22370; -.
DR TAIR; locus:2176357; AT5G22370.
DR eggNOG; KOG1533; Eukaryota.
DR HOGENOM; CLU_037460_0_2_1; -.
DR InParanoid; Q56XY2; -.
DR OMA; YAPLPFN; -.
DR OrthoDB; 964931at2759; -.
DR PhylomeDB; Q56XY2; -.
DR PRO; PR:Q56XY2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q56XY2; baseline and differential.
DR GO; GO:0005874; C:microtubule; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0009524; C:phragmoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IMP:TAIR.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR GO; GO:0032502; P:developmental process; IMP:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:1902182; P:shoot apical meristem development; IMP:TAIR.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR004130; Gpn.
DR InterPro; IPR030231; Gpn2.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR21231; PTHR21231; 1.
DR PANTHER; PTHR21231:SF3; PTHR21231:SF3; 1.
DR Pfam; PF03029; ATP_bind_1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; GTP-binding; Hydrolase; Nucleotide-binding;
KW Nucleus; Reference proteome.
FT CHAIN 1..298
FT /note="GPN-loop GTPase QQT1"
FT /id="PRO_0000443286"
FT MOTIF 69..71
FT /note="Gly-Pro-Asn (GPN)-loop; involved in dimer interface"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT BINDING 12..17
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT BINDING 173..176
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
FT SITE 71
FT /note="Stabilizes the phosphate intermediate; shared with
FT dimeric partner"
FT /evidence="ECO:0000250|UniProtKB:Q9UYR9"
SQ SEQUENCE 298 AA; 33565 MW; 35AB2A090D5CE7A4 CRC64;
MVFGQVVIGP PGSGKTTYCN GMSQFLSLMG RKVAIVNLDP ANDALPYECG VNIEELIKLE
DVMSEHSLGP NGGLVYCMEY LEKNIDWLES KLKPLLKDHY ILFDFPGQVE LFFIHDSTKN
VLTKLIKSLN LRLTAVQLID SHLCCDPGNY VSSLLLSLST MLHMELPHVN VLSKIDLIGS
YGKLAFNLDF YTDVQDLSYL EHHLSQDPRS AKYRKLTKEL CSVIEDYSLV NFTTLDIQDK
ESVGDLVKLI DKSNGYIFAG IDASVVEYSK IAIGQTDWDY NRVAAVQEKY MEDEEIQD
