QRIC2_MOUSE
ID QRIC2_MOUSE Reviewed; 592 AA.
AC Q3V2A7; B9EKM0;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Glutamine-rich protein 2;
GN Name=Qrich2 {ECO:0000312|MGI:MGI:2684912};
GN Synonyms=Gm66 {ECO:0000312|MGI:MGI:2684912};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT 471-LEU--GLN-479 DEL.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND SUBCELLULAR LOCATION.
RX PubMed=30683861; DOI=10.1038/s41467-018-08182-x;
RA Shen Y., Zhang F., Li F., Jiang X., Yang Y., Li X., Li W., Wang X.,
RA Cheng J., Liu M., Zhang X., Yuan G., Pei X., Cai K., Hu F., Sun J., Yan L.,
RA Tang L., Jiang C., Tu W., Xu J., Wu H., Kong W., Li S., Wang K., Sheng K.,
RA Zhao X., Yue H., Yang X., Xu W.;
RT "Loss-of-function mutations in QRICH2 cause male infertility with multiple
RT morphological abnormalities of the sperm flagella.";
RL Nat. Commun. 10:433-433(2019).
CC -!- FUNCTION: Has an essential role in the formation of sperm flagella and
CC flagellar structure maintainance. It acts as a suppressor of
CC ubiquitination and degradation of proteins involved in flagellar
CC development and motility. {ECO:0000269|PubMed:30683861}.
CC -!- SUBUNIT: Interacts with AKAP3, ODF2 and TSSK4.
CC {ECO:0000250|UniProtKB:Q9H0J4}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:30683861}.
CC Nucleus {ECO:0000269|PubMed:30683861}. Cytoplasm
CC {ECO:0000269|PubMed:30683861}. Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:30683861}. Note=Localization varies during
CC spermatozoa development. The protein is distributed in the nuclear
CC membrane of the spermatogonia, in the nucleus of round spermatids, in
CC the nucleus and cytoplasm of early elongating spermatids, in the
CC cytoplasm of late elongating spermatids, and in the flagella of
CC epididymal spermatozoa. {ECO:0000269|PubMed:30683861}.
CC -!- TISSUE SPECIFICITY: Expressed in testis (PubMed:30683861). Not detected
CC in heart, brain, kidney, stomach, ovary, liver, lung and uterus.
CC {ECO:0000269|PubMed:30683861}.
CC -!- DEVELOPMENTAL STAGE: Significant expression begins at postnatal day 15
CC and reaches a peak at postnatal day 22. Stable and high expression is
CC detected after postnatal day 22. {ECO:0000269|PubMed:30683861}.
CC -!- DISRUPTION PHENOTYPE: Knockout mice show coiled, bent, irregular, short
CC or absent flagella, defects of sperm flagellar ultrastructure, a
CC significant reduction in sperm locomotion, and decreased sperm count.
CC {ECO:0000269|PubMed:30683861}.
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DR EMBL; AK131945; BAE20891.1; -; mRNA.
DR EMBL; AL645851; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL645861; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC150976; AAI50977.1; -; mRNA.
DR CCDS; CCDS25667.1; -.
DR RefSeq; NP_001028439.1; NM_001033267.2.
DR AlphaFoldDB; Q3V2A7; -.
DR SMR; Q3V2A7; -.
DR STRING; 10090.ENSMUSP00000091437; -.
DR PhosphoSitePlus; Q3V2A7; -.
DR ProteomicsDB; 343448; -.
DR Antibodypedia; 19682; 19 antibodies from 9 providers.
DR Ensembl; ENSMUST00000093909; ENSMUSP00000091437; ENSMUSG00000070331.
DR GeneID; 217341; -.
DR KEGG; mmu:217341; -.
DR UCSC; uc007mlc.1; mouse.
DR CTD; 84074; -.
DR MGI; MGI:2684912; Qrich2.
DR VEuPathDB; HostDB:ENSMUSG00000070331; -.
DR GeneTree; ENSGT00940000161294; -.
DR HOGENOM; CLU_010161_1_0_1; -.
DR BioGRID-ORCS; 217341; 3 hits in 71 CRISPR screens.
DR PRO; PR:Q3V2A7; -.
DR Proteomes; UP000000589; Chromosome 11.
DR Bgee; ENSMUSG00000070331; Expressed in spermatid and 17 other tissues.
DR ExpressionAtlas; Q3V2A7; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0036126; C:sperm flagellum; IDA:UniProtKB.
DR GO; GO:0030031; P:cell projection assembly; IMP:UniProtKB.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:UniProtKB.
DR GO; GO:2000059; P:negative regulation of ubiquitin-dependent protein catabolic process; IMP:UniProtKB.
DR InterPro; IPR032013; DUF4795.
DR Pfam; PF16043; DUF4795; 1.
PE 2: Evidence at transcript level;
KW Cell projection; Cilium; Cytoplasm; Flagellum; Membrane; Nucleus;
KW Reference proteome; Repeat.
FT CHAIN 1..592
FT /note="Glutamine-rich protein 2"
FT /id="PRO_0000447710"
FT REGION 488..592
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 506..524
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 540..568
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 471..479
FT /note="Missing"
FT /evidence="ECO:0000269|PubMed:15489334"
SQ SEQUENCE 592 AA; 66541 MW; 5259C3BDADD48C6A CRC64;
MENSVSEASL YLQDQLDKLR TIIESMLGSS STLLSMSITP HKSTACLVPG QIDPEATCPA
CSLDVSHQVS LLVQRYEQLQ DMVSGLAASR PSKKAKLQGQ DEELLGHVQS AILQVQGDCE
KLNITTSNLI EDHRQKQKDI EVLYQGIERL DKEKANREHL EMEIDEKADK SALASKVSRI
QFDATTEQLN HMMQELVAKM SGQEQDWQKL LDKLLAEMDS KLDRLELDPL KQMLEDRWKS
LRQQLKERPP LYQADEAAAM RRQLLAHFHC LSCDRPLETT VTGQVISVTP IISSMPGHRS
VRPYTVFELE QIRQQSRNLK LGSSFPRVDM SQMERSVGRL HSMHSRMLMD MEKVQVHFGG
SVKASSQMIR ELLHTQCLSH PCYKRGADTA DYSYSTVSRR CGGSHTLTYP YRRNRPQHLS
PLEEIQIAMK HDEVDILGLD GHIYKGRMDT RLPGILGKDA PGVTKHNKAK LQQLQQLQQL
QQLQQLQQLQ QAQHARPHAH RQPSLGNMIS PPSRPQSAQM IADSKAVPSG QKKDRPVSSE
GRLLQSNVSH SSIPTDIASL QGSQQGLNMH IDVPPGEGLE EPTRGPRSTA AH
