QUAI_DROME
ID QUAI_DROME Reviewed; 888 AA.
AC Q23989; Q540V4; Q8INY7; Q9VJC9;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Villin-like protein quail;
GN Name=qua; ORFNames=CG6433;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=8044841; DOI=10.1016/0092-8674(94)90298-4;
RA Mahajan-Miklos S., Cooley L.;
RT "The villin-like protein encoded by the Drosophila quail gene is required
RT for actin bundle assembly during oogenesis.";
RL Cell 78:291-301(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Required for the formation of cytoplasmic actin filament
CC bundles in nurse cells, possibly by regulating both the polymerization
CC and organization of actin filaments. Mutations in quail result in
CC female sterility due to the disruption of cytoplasmic transport from
CC the nurse cells into the oocyte late in oogenesis.
CC {ECO:0000269|PubMed:8044841}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=Q23989-1; Sequence=Displayed;
CC Name=A;
CC IsoId=Q23989-2; Sequence=VSP_034369;
CC -!- TISSUE SPECIFICITY: Germline specific in adult flies.
CC {ECO:0000269|PubMed:8044841}.
CC -!- SIMILARITY: Belongs to the villin/gelsolin family. {ECO:0000305}.
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DR EMBL; U10070; AAC13765.1; -; mRNA.
DR EMBL; AE014134; AAF53623.1; -; Genomic_DNA.
DR EMBL; AE014134; AAN10991.1; -; Genomic_DNA.
DR EMBL; AY121684; AAM52011.1; -; mRNA.
DR PIR; A54832; A54832.
DR RefSeq; NP_724060.1; NM_165222.2. [Q23989-2]
DR RefSeq; NP_724061.1; NM_165223.3. [Q23989-1]
DR AlphaFoldDB; Q23989; -.
DR SMR; Q23989; -.
DR BioGRID; 61054; 5.
DR IntAct; Q23989; 2.
DR STRING; 7227.FBpp0297295; -.
DR PaxDb; Q23989; -.
DR DNASU; 35058; -.
DR EnsemblMetazoa; FBtr0080996; FBpp0080549; FBgn0003187. [Q23989-2]
DR EnsemblMetazoa; FBtr0080997; FBpp0080550; FBgn0003187. [Q23989-1]
DR GeneID; 35058; -.
DR KEGG; dme:Dmel_CG6433; -.
DR CTD; 35058; -.
DR FlyBase; FBgn0003187; qua.
DR VEuPathDB; VectorBase:FBgn0003187; -.
DR eggNOG; KOG0443; Eukaryota.
DR InParanoid; Q23989; -.
DR PhylomeDB; Q23989; -.
DR Reactome; R-DME-264870; Caspase-mediated cleavage of cytoskeletal proteins.
DR Reactome; R-DME-6798695; Neutrophil degranulation.
DR SignaLink; Q23989; -.
DR BioGRID-ORCS; 35058; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 35058; -.
DR PRO; PR:Q23989; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0003187; Expressed in secondary oocyte and 32 other tissues.
DR ExpressionAtlas; Q23989; baseline and differential.
DR Genevisible; Q23989; DM.
DR GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0003779; F:actin binding; IDA:FlyBase.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; ISS:FlyBase.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0051014; P:actin filament severing; IBA:GO_Central.
DR GO; GO:0008154; P:actin polymerization or depolymerization; IBA:GO_Central.
DR GO; GO:0051016; P:barbed-end actin filament capping; IBA:GO_Central.
DR GO; GO:0035317; P:imaginal disc-derived wing hair organization; IMP:FlyBase.
DR GO; GO:0007300; P:ovarian nurse cell to oocyte transport; HMP:FlyBase.
DR Gene3D; 1.10.950.10; -; 1.
DR Gene3D; 3.40.20.10; -; 6.
DR InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf.
DR InterPro; IPR036180; Gelsolin-like_dom_sf.
DR InterPro; IPR030016; Quail.
DR InterPro; IPR007122; Villin/Gelsolin.
DR InterPro; IPR003128; Villin_headpiece.
DR InterPro; IPR036886; Villin_headpiece_dom_sf.
DR PANTHER; PTHR11977; PTHR11977; 1.
DR PANTHER; PTHR11977:SF57; PTHR11977:SF57; 1.
DR Pfam; PF02209; VHP; 1.
DR PRINTS; PR00597; GELSOLIN.
DR SMART; SM00262; GEL; 6.
DR SMART; SM00153; VHP; 1.
DR SUPFAM; SSF47050; SSF47050; 1.
DR SUPFAM; SSF82754; SSF82754; 1.
DR PROSITE; PS51089; HP; 1.
PE 2: Evidence at transcript level;
KW Actin capping; Actin-binding; Alternative splicing; Developmental protein;
KW Differentiation; Oogenesis; Reference proteome.
FT CHAIN 1..888
FT /note="Villin-like protein quail"
FT /id="PRO_0000218742"
FT REPEAT 307..366
FT /note="Gelsolin-like"
FT DOMAIN 823..888
FT /note="HP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00595"
FT VAR_SEQ 2..10
FT /note="ELSLSPKET -> PPFNFIKQ (in isoform A)"
FT /evidence="ECO:0000303|PubMed:12537569,
FT ECO:0000303|PubMed:8044841"
FT /id="VSP_034369"
SQ SEQUENCE 888 AA; 100960 MW; DEEDF0441A4D6A2D CRC64;
MELSLSPKET EIRPNTIPDL KVDATFRKVA KHAITFAIWK IDEDRLEAVQ RSHYGTFYDS
CAYIIYAASL SGHYANHETI TREQKPNVSL ERYIHYWLGK NVSEQNRSNV VHKIQELDSY
LGNISSIYRE TQNLESARFL SYFKKGYDVR SGALISAPQR PRLFQLYARK WLRSIEVATI
DWSHFNSDYV MVLQTDNLTY VWIGRSSSGI ERRSALDWVQ KHCSGSPITI VDDGYEQAMS
QEHKELWNTM LPLKKRMVCQ ASQLVSEYAD YNSNKFRIYK CNQRGRLHLD QLDVGMPAKD
DLSDAHGVYL LDNYGQSIWL WVGGQAPQAD ALSAMGNGRA FVKKKKYPDN TLVVRVLEGH
EPVEFKRLFA NWLNVWQENT RGHKPVSTKF GKLDAHSLCE RPKMAADTQL VDDGRGERVI
YRVFGDQVQE VPISKTVVFT TNASFVVKYS VQCATVVPAD LASVGIKTII YQWNGSEASV
ESISRADKFA KASFDGLKEP GMFVQLYEFD EPPHFLQIFE GKLIIRRGQR TEMPYNGNSN
ALLDTFLLKV YGDASYNAKA VEETHLSSIS SKDCYVIKTN HVWVWCGQSS TGDAREMAKA
VGALMGENSL VLEGKESKEF WQSVAMYFNQ TLVINGNGNS CSSSTSSSSG AGSMCNGSSN
GGNISPTLSN NCYLNTSVPS KPRPPVQLFL VWWQQSSLRY EEILGFDQQD LSSDCTYILD
TGSLTYVWLG SQAPNQERYT AIAQSYVQNA PFGRRSATAL AVVRQFQEPN VFKGFFESWQ
NDYGKNFHSY EKMRKDLGNK VTSNCCFASE GSALILNNRQ KDFDGHKKYP LTVLIQEMDM
LPPDINPLKR EVHLTHDDFV SVFNMSFYEF DELPKWKKME LKKQFKLF
