ID   QUEA_PSESM              Reviewed;         354 AA.
AC   Q9L6W9;
DT   24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=S-adenosylmethionine:tRNA ribosyltransferase-isomerase {ECO:0000255|HAMAP-Rule:MF_00113};
DE            EC=2.4.99.17 {ECO:0000255|HAMAP-Rule:MF_00113};
DE   AltName: Full=Queuosine biosynthesis protein QueA {ECO:0000255|HAMAP-Rule:MF_00113};
GN   Name=queA {ECO:0000255|HAMAP-Rule:MF_00113}; OrderedLocusNames=PSPTO_1412;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=10781092; DOI=10.1073/pnas.97.9.4856;
RA   Alfano J.R., Charkowski A.O., Deng W.-L., Badel J.L., Petnicki-Ocwieja T.,
RA   van Dijk K., Collmer A.;
RT   "The Pseudomonas syringae Hrp pathogenicity island has a tripartite mosaic
RT   structure composed of a cluster of type III secretion genes bounded by
RT   exchangeable effector and conserved effector loci that contribute to
RT   parasitic fitness and pathogenicity in plants.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:4856-4861(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RA   Ramos A.R., Rehm A.H., Collmer A.R.;
RT   "Pseudomonas syringae pv. tomato DC3000 hrpL through hrcU.";
RL   Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- FUNCTION: Transfers and isomerizes the ribose moiety from AdoMet to the
CC       7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give
CC       epoxyqueuosine (oQ-tRNA). {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-L-
CC         methionine = adenine + epoxyqueuosine(34) in tRNA + H(+) + L-
CC         methionine; Xref=Rhea:RHEA:32155, Rhea:RHEA-COMP:10342, Rhea:RHEA-
CC         COMP:10346, ChEBI:CHEBI:15378, ChEBI:CHEBI:16708, ChEBI:CHEBI:57844,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:82833, ChEBI:CHEBI:82834;
CC         EC=2.4.99.17; Evidence={ECO:0000255|HAMAP-Rule:MF_00113};
CC   -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SIMILARITY: Belongs to the QueA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00113}.
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DR   EMBL; AF232004; AAF71483.1; -; Genomic_DNA.
DR   EMBL; AE016853; AAO54933.1; -; Genomic_DNA.
DR   RefSeq; NP_791238.1; NC_004578.1.
DR   RefSeq; WP_005771042.1; NC_004578.1.
DR   AlphaFoldDB; Q9L6W9; -.
DR   SMR; Q9L6W9; -.
DR   STRING; 223283.PSPTO_1412; -.
DR   EnsemblBacteria; AAO54933; AAO54933; PSPTO_1412.
DR   GeneID; 1183049; -.
DR   KEGG; pst:PSPTO_1412; -.
DR   PATRIC; fig|223283.9.peg.1432; -.
DR   eggNOG; COG0809; Bacteria.
DR   HOGENOM; CLU_039110_1_0_6; -.
DR   OMA; YSYGDGM; -.
DR   OrthoDB; 368001at2; -.
DR   PhylomeDB; Q9L6W9; -.
DR   UniPathway; UPA00392; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051075; F:S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.10.240; -; 1.
DR   Gene3D; 3.40.1780.10; -; 1.
DR   HAMAP; MF_00113; QueA; 1.
DR   InterPro; IPR003699; QueA.
DR   InterPro; IPR042118; QueA_dom1.
DR   InterPro; IPR042119; QueA_dom2.
DR   InterPro; IPR036100; QueA_sf.
DR   PANTHER; PTHR30307; PTHR30307; 1.
DR   Pfam; PF02547; Queuosine_synth; 1.
DR   SUPFAM; SSF111337; SSF111337; 1.
DR   TIGRFAMs; TIGR00113; queA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Queuosine biosynthesis; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..354
FT                   /note="S-adenosylmethionine:tRNA ribosyltransferase-
FT                   isomerase"
FT                   /id="PRO_0000165427"
SQ   SEQUENCE   354 AA;  38719 MW;  AE6484EA1F98FB23 CRC64;
     MRVADFTFEL PDSLIARHPL AERRSSRLLT LDGPTGALAH RQFTDLLEHL RSGDLMVFNN
     TRVIPARLFG QKASGGKLEI LVERVLDSHR VLAHVRASKS PKPGSSILID GGGEAEMVAR
     HDALFELRFA EEVLPLLDRV GHMPLPPYID RPDEGADRER YQTVYAQRAG AVAAPTAGLH
     FDQPLMEAIA AKGVETAFVT LHVGAGTFQP VRVEQIEDHH MHSEWLEVSQ DVVDAVAACR
     ARGGRVIAVG TTSVRSLESA ARDGQLKPFS GDTDIFIYPG RPFHVVDALV TNFHLPESTL
     LMLVSAFAGY PETMAAYAAA IEHGYRFFSY GDAMFITRNP APTAPQESAP EDHA