ID   QUEA_PSEU2              Reviewed;         354 AA.
AC   Q9L6W2; Q4ZX44;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 2.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=S-adenosylmethionine:tRNA ribosyltransferase-isomerase {ECO:0000255|HAMAP-Rule:MF_00113};
DE            EC=2.4.99.17 {ECO:0000255|HAMAP-Rule:MF_00113};
DE   AltName: Full=Queuosine biosynthesis protein QueA {ECO:0000255|HAMAP-Rule:MF_00113};
GN   Name=queA {ECO:0000255|HAMAP-Rule:MF_00113}; OrderedLocusNames=Psyr_1227;
OS   Pseudomonas syringae pv. syringae (strain B728a).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=205918;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10781092; DOI=10.1073/pnas.97.9.4856;
RA   Alfano J.R., Charkowski A.O., Deng W.-L., Badel J.L., Petnicki-Ocwieja T.,
RA   van Dijk K., Collmer A.;
RT   "The Pseudomonas syringae Hrp pathogenicity island has a tripartite mosaic
RT   structure composed of a cluster of type III secretion genes bounded by
RT   exchangeable effector and conserved effector loci that contribute to
RT   parasitic fitness and pathogenicity in plants.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:4856-4861(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B728a;
RX   PubMed=16043691; DOI=10.1073/pnas.0504930102;
RA   Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A.,
RA   Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S.,
RA   Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M.,
RA   Kyrpides N.C., Ivanova N., Lindow S.E.;
RT   "Comparison of the complete genome sequences of Pseudomonas syringae pv.
RT   syringae B728a and pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005).
CC   -!- FUNCTION: Transfers and isomerizes the ribose moiety from AdoMet to the
CC       7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give
CC       epoxyqueuosine (oQ-tRNA). {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-L-
CC         methionine = adenine + epoxyqueuosine(34) in tRNA + H(+) + L-
CC         methionine; Xref=Rhea:RHEA:32155, Rhea:RHEA-COMP:10342, Rhea:RHEA-
CC         COMP:10346, ChEBI:CHEBI:15378, ChEBI:CHEBI:16708, ChEBI:CHEBI:57844,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:82833, ChEBI:CHEBI:82834;
CC         EC=2.4.99.17; Evidence={ECO:0000255|HAMAP-Rule:MF_00113};
CC   -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SIMILARITY: Belongs to the QueA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00113}.
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DR   EMBL; AF232005; AAF71490.1; -; Genomic_DNA.
DR   EMBL; CP000075; AAY36278.1; -; Genomic_DNA.
DR   RefSeq; WP_011266902.1; NC_007005.1.
DR   RefSeq; YP_234316.1; NC_007005.1.
DR   AlphaFoldDB; Q9L6W2; -.
DR   SMR; Q9L6W2; -.
DR   STRING; 205918.Psyr_1227; -.
DR   EnsemblBacteria; AAY36278; AAY36278; Psyr_1227.
DR   KEGG; psb:Psyr_1227; -.
DR   PATRIC; fig|205918.7.peg.1259; -.
DR   eggNOG; COG0809; Bacteria.
DR   HOGENOM; CLU_039110_1_0_6; -.
DR   OMA; YSYGDGM; -.
DR   UniPathway; UPA00392; -.
DR   Proteomes; UP000000426; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051075; F:S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.10.240; -; 1.
DR   Gene3D; 3.40.1780.10; -; 1.
DR   HAMAP; MF_00113; QueA; 1.
DR   InterPro; IPR003699; QueA.
DR   InterPro; IPR042118; QueA_dom1.
DR   InterPro; IPR042119; QueA_dom2.
DR   InterPro; IPR036100; QueA_sf.
DR   PANTHER; PTHR30307; PTHR30307; 1.
DR   Pfam; PF02547; Queuosine_synth; 1.
DR   SUPFAM; SSF111337; SSF111337; 1.
DR   TIGRFAMs; TIGR00113; queA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Queuosine biosynthesis; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..354
FT                   /note="S-adenosylmethionine:tRNA ribosyltransferase-
FT                   isomerase"
FT                   /id="PRO_0000165428"
FT   CONFLICT        59
FT                   /note="N -> T (in Ref. 1; AAF71490)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   354 AA;  38721 MW;  B10435EBFFA8A40D CRC64;
     MRVADFTFEL PDSLIARHPL AERRSSRLLT LDGPTGALAH RQFTDLLEHL RPGDLMVFNN
     TRVIPARLFG QKASGGKLEI LVERVLDSHR VLAHVRSSKS PKPGSSILID GGGEAEMVAR
     HDALFELRFA EEVLPLLERV GRMPLPPYID RPDEGADRER YQTVYAQRAG AVAAPTAGLH
     FDQPLLEAIA AKGVETAFVT LHVGAGTFQP VRVEQIEDHH MHSEWLEVGQ DVVDAVAACR
     ARGGRVIAVG TTSVRSLESA ARDGELKSFS GDTDIFIYPG RPFHVVDALV TNFHLPESTL
     LMLVSAFAGY PETMAAYAAA IEHGYRFFSY GDAMFITRNP APTAPQESAP EDHA