QUEC_ECOLI
ID QUEC_ECOLI Reviewed; 231 AA.
AC P77756; Q2MBY2;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=7-cyano-7-deazaguanine synthase;
DE EC=6.3.4.20;
DE AltName: Full=7-cyano-7-carbaguanine synthase;
DE AltName: Full=PreQ(0) synthase;
DE AltName: Full=Queuosine biosynthesis protein QueC;
GN Name=queC; Synonyms=ybaX; OrderedLocusNames=b0444, JW0434;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Hatada E., Ohmori H., Qiao Y., Tsuji M., Fukuda R.;
RT "Nucleotide sequence analysis of the E. coli chromosome around the 10.0 min
RT region.";
RL Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION IN QUEUOSINE BIOSYNTHESIS.
RC STRAIN=ATCC 33694 / HB101;
RX PubMed=16199558; DOI=10.1128/jb.187.20.6893-6901.2005;
RA Gaur R., Varshney U.;
RT "Genetic analysis identifies a function for the queC (ybaX) gene product at
RT an initial step in the queuosine biosynthetic pathway in Escherichia
RT coli.";
RL J. Bacteriol. 187:6893-6901(2005).
CC -!- FUNCTION: Catalyzes the ATP-dependent conversion of 7-carboxy-7-
CC deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). {ECO:0000250,
CC ECO:0000269|PubMed:16199558}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-
CC deazaguanine + ADP + H(+) + H2O + phosphate; Xref=Rhea:RHEA:27982,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:45075,
CC ChEBI:CHEBI:61036, ChEBI:CHEBI:456216; EC=6.3.4.20;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- PATHWAY: Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
CC -!- INTERACTION:
CC P77756; P0CE47: tufA; NbExp=3; IntAct=EBI-560024, EBI-301077;
CC -!- SIMILARITY: Belongs to the QueC family. {ECO:0000305}.
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DR EMBL; D82943; BAA11648.1; -; Genomic_DNA.
DR EMBL; U82664; AAB40200.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73547.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76224.1; -; Genomic_DNA.
DR PIR; D64774; D64774.
DR RefSeq; NP_414978.1; NC_000913.3.
DR RefSeq; WP_000817220.1; NZ_SSZK01000009.1.
DR AlphaFoldDB; P77756; -.
DR SMR; P77756; -.
DR BioGRID; 4262187; 62.
DR DIP; DIP-11311N; -.
DR IntAct; P77756; 14.
DR STRING; 511145.b0444; -.
DR jPOST; P77756; -.
DR PaxDb; P77756; -.
DR PRIDE; P77756; -.
DR EnsemblBacteria; AAC73547; AAC73547; b0444.
DR EnsemblBacteria; BAE76224; BAE76224; BAE76224.
DR GeneID; 947034; -.
DR KEGG; ecj:JW0434; -.
DR KEGG; eco:b0444; -.
DR PATRIC; fig|1411691.4.peg.1832; -.
DR EchoBASE; EB3041; -.
DR eggNOG; COG0603; Bacteria.
DR HOGENOM; CLU_081854_0_0_6; -.
DR InParanoid; P77756; -.
DR OMA; CESCMRR; -.
DR PhylomeDB; P77756; -.
DR BioCyc; EcoCyc:G6245-MON; -.
DR BioCyc; MetaCyc:G6245-MON; -.
DR UniPathway; UPA00391; -.
DR PRO; PR:P77756; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008616; P:queuosine biosynthetic process; IBA:GO_Central.
DR CDD; cd01995; ExsB; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_01633; QueC; 1.
DR InterPro; IPR018317; QueC.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR42914; PTHR42914; 1.
DR Pfam; PF06508; QueC; 1.
DR PIRSF; PIRSF006293; ExsB; 1.
DR TIGRFAMs; TIGR00364; TIGR00364; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Ligase; Metal-binding; Nucleotide-binding;
KW Queuosine biosynthesis; Reference proteome; Zinc.
FT CHAIN 1..231
FT /note="7-cyano-7-deazaguanine synthase"
FT /id="PRO_0000168631"
FT BINDING 8..18
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 188
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 197
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 200
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 203
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
SQ SEQUENCE 231 AA; 25514 MW; 69E20ED03E6E3E34 CRC64;
MKRAVVVFSG GQDSTTCLVQ ALQQYDEVHC VTFDYGQRHR AEIDVARELA LKLGARAHKV
LDVTLLNELA VSSLTRDSIP VPDYEPEADG IPNTFVPGRN ILFLTLAAIY AYQVKAEAVI
TGVCETDFSG YPDCRDEFVK ALNHAVSLGM AKDIRFETPL MWIDKAETWA LADYYGKLDL
VRNETLTCYN GFKGDGCGHC AACNLRANGL NHYLADKPTV MAAMKQKTGL R
