R12BA_MOUSE
ID R12BA_MOUSE Reviewed; 836 AA.
AC Q80YR9; B1AZ74; Q7TQK7; Q8CD70; Q9CRY6;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=RNA-binding protein 12B-A;
DE AltName: Full=RNA-binding motif protein 12B-A;
GN Name=Rbm12b1; Synonyms=Rbm12b, Rbm12ba;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-82 AND 114-836.
RC STRAIN=C57BL/6J; TISSUE=Head, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 239-836.
RC TISSUE=Limb, and Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-703, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-758, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH54081.1; Type=Miscellaneous discrepancy; Note=Possible contaminating sequence. The N-terminal 4 residues do not match the underlying genomic sequence.; Evidence={ECO:0000305};
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DR EMBL; AL929413; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK013872; BAB29027.1; -; mRNA.
DR EMBL; AK031339; BAC27354.1; -; mRNA.
DR EMBL; BC050844; AAH50844.1; -; mRNA.
DR EMBL; BC054081; AAH54081.1; ALT_SEQ; mRNA.
DR CCDS; CCDS17976.1; -.
DR RefSeq; NP_082502.2; NM_028226.2.
DR RefSeq; XP_006538334.1; XM_006538271.3.
DR RefSeq; XP_006538335.1; XM_006538272.3.
DR RefSeq; XP_006538336.1; XM_006538273.3.
DR RefSeq; XP_006538337.1; XM_006538274.2.
DR AlphaFoldDB; Q80YR9; -.
DR STRING; 10090.ENSMUSP00000064195; -.
DR iPTMnet; Q80YR9; -.
DR PhosphoSitePlus; Q80YR9; -.
DR EPD; Q80YR9; -.
DR jPOST; Q80YR9; -.
DR MaxQB; Q80YR9; -.
DR PaxDb; Q80YR9; -.
DR PeptideAtlas; Q80YR9; -.
DR PRIDE; Q80YR9; -.
DR ProteomicsDB; 300221; -.
DR DNASU; 72397; -.
DR Ensembl; ENSMUST00000050069; ENSMUSP00000053555; ENSMUSG00000046667.
DR Ensembl; ENSMUST00000069128; ENSMUSP00000064195; ENSMUSG00000046667.
DR GeneID; 72397; -.
DR KEGG; mmu:72397; -.
DR UCSC; uc008san.3; mouse.
DR CTD; 72397; -.
DR MGI; MGI:1919647; Rbm12b1.
DR VEuPathDB; HostDB:ENSMUSG00000046667; -.
DR eggNOG; KOG4307; Eukaryota.
DR GeneTree; ENSGT00940000158322; -.
DR HOGENOM; CLU_004368_1_0_1; -.
DR InParanoid; Q80YR9; -.
DR OMA; DRDDHSH; -.
DR OrthoDB; 249376at2759; -.
DR PhylomeDB; Q80YR9; -.
DR TreeFam; TF331899; -.
DR BioGRID-ORCS; 72397; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Rbm12b1; mouse.
DR PRO; PR:Q80YR9; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q80YR9; protein.
DR Bgee; ENSMUSG00000046667; Expressed in embryonic post-anal tail and 223 other tissues.
DR Genevisible; Q80YR9; MM.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0043484; P:regulation of RNA splicing; IBA:GO_Central.
DR CDD; cd12746; RRM2_RBM12B; 1.
DR CDD; cd12513; RRM3_RBM12B; 1.
DR Gene3D; 3.30.70.330; -; 5.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR034588; RBM12B_RRM2.
DR InterPro; IPR034858; RBM12B_RRM3.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 3.
DR SMART; SM00360; RRM; 5.
DR SUPFAM; SSF54928; SSF54928; 4.
DR PROSITE; PS50102; RRM; 4.
PE 1: Evidence at protein level;
KW Acetylation; Phosphoprotein; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..836
FT /note="RNA-binding protein 12B-A"
FT /id="PRO_0000273368"
FT DOMAIN 154..229
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 283..359
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 401..478
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 760..836
FT /note="RRM 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 237..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 539..572
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 620..644
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 259..274
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 546..572
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 703
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 758
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT CONFLICT 592
FT /note="S -> P (in Ref. 2; AAH50844)"
FT /evidence="ECO:0000305"
FT CONFLICT 605
FT /note="R -> H (in Ref. 2; AAH50844)"
FT /evidence="ECO:0000305"
FT CONFLICT 637
FT /note="E -> Q (in Ref. 2; AAH50844)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 836 AA; 96596 MW; C39C6A45A6BA606A CRC64;
MAVVIRLLGL PFIAGPVDIR HFFKGLTIPD GGVHIIGGKV GEAFIIFATD EDARRAISRS
GGFIKDSSVE LFLSSKVEMQ KTIEMKRTAR VGRGRPGSGA SGVGNVYHFS DALKEEESYS
GYGSSVNRDA GFHTNGTGLD LRPRKTRPLK AENPYLFLRG LPYLVNEDDV RVFFSGLCVD
GVILLKHHDG RNNGDAIVKF ASCVDASGGL KCHRSFMGSR FIEVMQGSEQ QWIEFGGTAT
EGGDTPRMRS EEHSPSRRIN GRHFRKRSHS KSPRARSRSP LGFYVHLKNL SLNTNKRDLR
NLFRDTDLTN DQIKFVYKDE RRTRYAFVMF KNQKDYNTAL GLHKTVLQYR PVLIDPVSRK
EMVRIIECYE KKRPESLEKE RPGRVSQKYS QEGFSGSGQK LCIYIRNLPF DVTKGEVQKF
FADFSLVEDD IYLLCDDKGV GLGEALVRFK SEEQAMKAER LNRQRFLGIE VLLRLISEEQ
MQEFGVKSSW LSNERTQACS RSHDGDDCSC LFDLKDPSSC SFGQSESLRY HPKDLRKMGH
FKHPQGYFRQ SDRRSPEDFR HSPEDYRHPW EEHTSHSREE DWRLPLEDWK WSQEDDFRQC
HEKDRRQLRS PWEEDFRRPS QEHFRRSYQE HIRQPPEEHF RRSREEDFRH VADEDFRQAS
DEDFRTSQED LRYPTDEDFR RVSVEDLREV PEKDLRLPKN FRSPGEEFWT PPDFRGQHPF
GNFDHLQGGK FDFEKYKFGS FPDAKVTSDL NLNCVSDKII PVKISNLPFK ANASEILDFF
HGYKVIPDSV SLQYNEQGLP IGEAIVAMTN YNEALAAVKD LSGRPVGPRK VKLSLL
