R12BB_MOUSE
ID R12BB_MOUSE Reviewed; 834 AA.
AC Q66JV4; A2AJP4; Q3TE05; Q8BNP2; Q8K0T9;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=RNA-binding protein 12B-B;
DE AltName: Full=RNA-binding motif protein 12B-B;
GN Name=Rbm12b2; Synonyms=Rbm12bb;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Adipose tissue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Embryonic germ cell, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q66JV4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q66JV4-2; Sequence=VSP_022525;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH30408.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK080943; BAC38085.1; -; mRNA.
DR EMBL; AK169895; BAE41443.1; -; mRNA.
DR EMBL; AL772167; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC030408; AAH30408.1; ALT_INIT; mRNA.
DR EMBL; BC080741; AAH80741.1; -; mRNA.
DR CCDS; CCDS17975.1; -. [Q66JV4-1]
DR RefSeq; NP_945195.1; NM_198957.2. [Q66JV4-1]
DR RefSeq; XP_011248435.1; XM_011250133.1.
DR RefSeq; XP_017175942.1; XM_017320453.1. [Q66JV4-1]
DR RefSeq; XP_017175943.1; XM_017320454.1.
DR AlphaFoldDB; Q66JV4; -.
DR STRING; 10090.ENSMUSP00000066311; -.
DR iPTMnet; Q66JV4; -.
DR PhosphoSitePlus; Q66JV4; -.
DR EPD; Q66JV4; -.
DR jPOST; Q66JV4; -.
DR MaxQB; Q66JV4; -.
DR PaxDb; Q66JV4; -.
DR PeptideAtlas; Q66JV4; -.
DR PRIDE; Q66JV4; -.
DR ProteomicsDB; 300300; -. [Q66JV4-1]
DR ProteomicsDB; 300301; -. [Q66JV4-2]
DR Ensembl; ENSMUST00000063839; ENSMUSP00000066311; ENSMUSG00000052137. [Q66JV4-1]
DR Ensembl; ENSMUST00000095143; ENSMUSP00000092765; ENSMUSG00000052137. [Q66JV4-1]
DR GeneID; 77604; -.
DR KEGG; mmu:77604; -.
DR UCSC; uc008sak.1; mouse. [Q66JV4-1]
DR CTD; 77604; -.
DR MGI; MGI:1924854; Rbm12b2.
DR VEuPathDB; HostDB:ENSMUSG00000052137; -.
DR eggNOG; KOG4307; Eukaryota.
DR GeneTree; ENSGT00940000158322; -.
DR HOGENOM; CLU_004368_1_0_1; -.
DR InParanoid; Q66JV4; -.
DR OMA; WRQPPEG; -.
DR OrthoDB; 249376at2759; -.
DR PhylomeDB; Q66JV4; -.
DR TreeFam; TF331899; -.
DR BioGRID-ORCS; 77604; 3 hits in 70 CRISPR screens.
DR PRO; PR:Q66JV4; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q66JV4; protein.
DR Bgee; ENSMUSG00000052137; Expressed in manus and 199 other tissues.
DR Genevisible; Q66JV4; MM.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0043484; P:regulation of RNA splicing; IBA:GO_Central.
DR CDD; cd12746; RRM2_RBM12B; 1.
DR CDD; cd12513; RRM3_RBM12B; 1.
DR Gene3D; 3.30.70.330; -; 5.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR034588; RBM12B_RRM2.
DR InterPro; IPR034858; RBM12B_RRM3.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 3.
DR SMART; SM00360; RRM; 5.
DR SUPFAM; SSF54928; SSF54928; 4.
DR PROSITE; PS50102; RRM; 4.
PE 2: Evidence at transcript level;
KW Alternative splicing; Phosphoprotein; Reference proteome; Repeat;
KW RNA-binding.
FT CHAIN 1..834
FT /note="RNA-binding protein 12B-B"
FT /id="PRO_0000273369"
FT DOMAIN 154..229
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 283..359
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 401..478
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 758..834
FT /note="RRM 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 237..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 546..572
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 621..643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 259..274
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 553..572
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 701
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q80YR9"
FT VAR_SEQ 1..216
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_022525"
FT CONFLICT 167
FT /note="E -> D (in Ref. 3; AAH80741)"
FT /evidence="ECO:0000305"
FT CONFLICT 432
FT /note="Y -> H (in Ref. 1; BAE41443)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 834 AA; 96450 MW; AC6565109383C6AD CRC64;
MAVVIRLLGL PFIAGPVDIR HFFKGLTIPD GGVHIIGGKV GEAFIIFATD EDARRAISRS
GGFIKDSSVE LFLSSKVEMQ KTIEMKRTAR VGRGRPGSGA SGVGNVYHFS DALKEEESYS
GYGSSVNRDA GFHTNGTGLD LRPRKTRPLK AENPYLFLRG LPYLVNEDDV RVFFSGLCVD
GVILLKHHDG RNNGDAIVKF ASCVDASGGL KCHRSFMGSR FIEVMQGSEQ QWIEFGGTAT
EGGDTPRMRS EEHSPSRRIN GRHFRKRSHS KSPRARSRSP LGFYVHLKNL SLNTNKRDLR
NLFRDTDLTN DQIKFVYKDE RRTRYAFVMF KNQKDYNTAL GLHKTVLQYR PVLIDPVSRK
EMVRIIECYE KKRPESLEKE RPGRVSQKYS QEGFSGSGQK LCIYIRNLPF DVTKGEVQKF
FADFSLVEDD IYLLCDDKGV GLGEALVRFK SEEQAMKAER LNRQRFLGIE VLLRLISEEQ
MQEFGIKSSW LSNERTQACS RSHDGDDCSC LFDLKDPSSC SFGQSESLRY HPKDLRKMGH
FKHPQGYFRQ SDRCSPEDFR HSPEDYRHPW EEHTSHSREE DWRLPLEDWP QEDDFRQCHE
KDHRQLRSPW EEDFRRPSQE HFRRSYQEHI RRPPQEHFRR SREEDFRHVA DEDFRQASDE
DFRISQEDLR YPTDEDFRRV SVEDLREVPE KDLRLPKNFR SSGEEFWTPP DFRGQHPFVN
FDHLQGGKFD FEKYKLENFH DGKFVPDLKF NCGSGGIIRV MISNLPFKAN ANEILDFFHG
YKVIPDSVSI QYNEEGLPLG EAIVSMTNYN EALSAVKDLS GRPVGPRKVK LSLL
