ID   R1AB_CVCAI              Reviewed;         168 AA.
AC   P36697;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Replicase polyprotein 1ab;
DE            Short=pp1ab;
DE   AltName: Full=ORF1ab polyprotein;
DE   Contains:
DE     RecName: Full=Putative 2'-O-methyl transferase;
DE              EC=2.1.1.-;
DE     AltName: Full=nsp16;
DE   Flags: Fragment;
GN   Name=rep; ORFNames=1a-1b;
OS   Canine coronavirus (strain Insavc-1) (CCoV) (Canine enteric coronavirus).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Tegacovirus.
OX   NCBI_TaxID=36391;
OH   NCBI_TaxID=9615; Canis lupus familiaris (Dog) (Canis familiaris).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1431811; DOI=10.1099/0022-1317-73-11-2849;
RA   Horsburgh B.C., Brierley I., Brown T.D.K.;
RT   "Analysis of a 9.6 kb sequence from the 3' end of canine coronavirus
RT   genomic RNA.";
RL   J. Gen. Virol. 73:2849-2862(1992).
CC   -!- FUNCTION: The replicase polyprotein of coronaviruses is a
CC       multifunctional protein: it contains the activities necessary for the
CC       transcription of negative stranded RNA, leader RNA, subgenomic mRNAs
CC       and progeny virion RNA as well as proteinases responsible for the
CC       cleavage of the polyprotein into functional products.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Ribosomal frameshifting; Named isoforms=2;
CC       Name=Replicase polyprotein 1ab; Synonyms=pp1ab;
CC         IsoId=P36697-1; Sequence=Displayed;
CC       Name=Replicase polyprotein 1a; Synonyms=pp1a, ORF1a polyprotein;
CC         IsoId=P36697-2; Sequence=Not described;
CC   -!- MISCELLANEOUS: [Isoform Replicase polyprotein 1ab]: Produced by -1
CC       ribosomal frameshifting at the 1a-1b genes boundary.
CC   -!- MISCELLANEOUS: [Isoform Replicase polyprotein 1a]: Produced by
CC       conventional translation. {ECO:0000305}.
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DR   EMBL; D13096; BAA02407.1; -; Genomic_RNA.
DR   PIR; PQ0481; PQ0481.
DR   SMR; P36697; -.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR009461; NSP16_CoV-like.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF06460; CoV_Methyltr_2; 1.
DR   PROSITE; PS51955; NIV_2_O_MTASE; 1.
PE   4: Predicted;
KW   Methyltransferase; Ribosomal frameshifting; Transferase.
FT   CHAIN           <1..168
FT                   /note="Putative 2'-O-methyl transferase"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000037289"
FT   DOMAIN          <1..165
FT                   /note="Nidovirus-type SAM-dependent 2'-O-MTase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01300"
FT   NON_TER         1
SQ   SEQUENCE   168 AA;  18980 MW;  B6FDD920BEA746B8 CRC64;
     PNTKSIDGEN TSKDGFFTYV NGFIKEKLSL GGSAAIKITE FSWNKDLYEL IQRFEYWTVF
     CTSVNTSSSE GFLIGVNYLG PYCDRAIVDG NIMHANYIFW RNSTIMALSH NSVLDTPKFK
     CRCNNALIVN LKEKELNEMV IGLLKKGKLL IRNNGKLLNF GNHLVNVP