R1AB_CVPFS
ID R1AB_CVPFS Reviewed; 533 AA.
AC P18457;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Replicase polyprotein 1ab;
DE Short=pp1ab;
DE AltName: Full=ORF1ab polyprotein;
DE Contains:
DE RecName: Full=Uridylate-specific endoribonuclease;
DE EC=3.1.-.-;
DE AltName: Full=NendoU;
DE AltName: Full=nsp15;
DE AltName: Full=p41;
DE Contains:
DE RecName: Full=Putative 2'-O-methyl transferase;
DE EC=2.1.1.-;
DE AltName: Full=nsp16;
DE Flags: Fragment;
GN Name=rep; ORFNames=1a-1b;
OS Porcine transmissible gastroenteritis coronavirus (strain FS772/70) (TGEV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC Alphacoronavirus; Tegacovirus.
OX NCBI_TaxID=11150;
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=1964522; DOI=10.1016/0168-1702(90)90090-x;
RA Britton P., Page K.W.;
RT "Sequence of the S gene from a virulent British field isolate of
RT transmissible gastroenteritis virus.";
RL Virus Res. 18:71-80(1990).
CC -!- FUNCTION: The replicase polyprotein of coronaviruses is a
CC multifunctional protein: it contains the activities necessary for the
CC transcription of negative stranded RNA, leader RNA, subgenomic mRNAs
CC and progeny virion RNA as well as proteinases responsible for the
CC cleavage of the polyprotein into functional products.
CC -!- FUNCTION: Nsp9 is a ssRNA-binding protein. {ECO:0000250}.
CC -!- FUNCTION: NendoU is a Mn(2+)-dependent, uridylate-specific enzyme,
CC which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Ribosomal frameshifting; Named isoforms=2;
CC Name=Replicase polyprotein 1ab; Synonyms=pp1ab;
CC IsoId=P18457-1; Sequence=Displayed;
CC Name=Replicase polyprotein 1a; Synonyms=pp1a, ORF1a polyprotein;
CC IsoId=P18457-2; Sequence=Not described;
CC -!- MISCELLANEOUS: [Isoform Replicase polyprotein 1ab]: Produced by -1
CC ribosomal frameshifting at the 1a-1b genes boundary.
CC -!- MISCELLANEOUS: [Isoform Replicase polyprotein 1a]: Produced by
CC conventional translation. {ECO:0000305}.
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DR EMBL; X53128; CAA37284.1; -; Genomic_RNA.
DR PIR; A43489; A43489.
DR SMR; P18457; -.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd20762; capping_2-OMTase_Nidovirales; 1.
DR CDD; cd21161; NendoU_cv_Nsp15-like; 1.
DR Gene3D; 3.40.50.11580; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR043608; CoV_NSP15_M.
DR InterPro; IPR037227; EndoU-like.
DR InterPro; IPR043609; NendoU_nidovirus.
DR InterPro; IPR043174; NSP15_middle_sf.
DR InterPro; IPR044401; NSP15_NendoU_CoV.
DR InterPro; IPR009461; NSP16_CoV-like.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF06460; CoV_Methyltr_2; 1.
DR Pfam; PF19215; CoV_NSP15_C; 1.
DR Pfam; PF19216; CoV_NSP15_M; 1.
DR SUPFAM; SSF142877; SSF142877; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS51961; AV_NSP11N_COV_NSP15M; 1.
DR PROSITE; PS51958; NENDOU; 1.
DR PROSITE; PS51955; NIV_2_O_MTASE; 1.
PE 3: Inferred from homology;
KW Endonuclease; Hydrolase; Methyltransferase; Nuclease;
KW Ribosomal frameshifting; Transferase.
FT CHAIN <1..233
FT /note="Uridylate-specific endoribonuclease"
FT /evidence="ECO:0000250"
FT /id="PRO_0000037384"
FT CHAIN 234..533
FT /note="Putative 2'-O-methyl transferase"
FT /evidence="ECO:0000250"
FT /id="PRO_0000037385"
FT DOMAIN <1..73
FT /note="AV-Nsp11N/CoV-Nsp15M"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01306"
FT DOMAIN 90..230
FT /note="NendoU"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01303"
FT DOMAIN 234..530
FT /note="Nidovirus-type SAM-dependent 2'-O-MTase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01300"
FT ACT_SITE 120
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01303"
FT ACT_SITE 135
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01303"
FT ACT_SITE 176
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01303"
FT ACT_SITE 278
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01300"
FT ACT_SITE 362
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01300"
FT ACT_SITE 402
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01300"
FT ACT_SITE 435
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01300"
FT SITE 233..234
FT /note="Cleavage; by 3CL-PRO"
FT /evidence="ECO:0000250"
FT NON_TER 1
SQ SEQUENCE 533 AA; 60270 MW; B1AE8C49DBA7FC5E CRC64;
LDSEVVTCFD NSIAGSFERF TTTRDAVLIS NNAVKGLSAI KLQYGLLNDL PVSTVGNKPV
TWYIYVRKNG EYVEQIDSYY TQGRTFETFK PRSTMEEDFL SMDTTLFIQK YGLEDYGFEH
VVFGDVSKTT IGGMHLLISQ VRLAKMGLFS VQEFMNNSDS TLKSCCITYA DDPSSKNVCT
YMDILLDDFV TIIKSLDLNV VSKVVDVIVD CKAWKWMLWC ENSHIKTFYP QLQSAEWNPG
YSMPTLYKIQ RMCLERCNLY NYGAQVKLPD GITTNVVKYT QLCQYLNTTT LCVPHKMRVL
HLGAAGASGV APGSTVLRRW LPDDAILVDN DLRDYVSDAD FSVTGDCTSL YIEDKFDLLV
SDLYDGSTKS IDGENTSKDG FFTYINGFIK EKLSLGGSVA IKITEFSWNK DLYELIQRFE
YWTVFCTSVN TSSSEGFLIG INYLGPYCDK AIVDGNIMHA NYIFWRNSTI MALSHNSVLD
TPKFKCRCNN ALIVNLKEKE LNEMVIGLLR KGKLLIRNNG KLLNFGNHFV NTP
