ID   R1AB_CVPR8              Reviewed;         131 AA.
AC   Q04158;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Replicase polyprotein 1ab;
DE            Short=pp1ab;
DE   AltName: Full=ORF1ab polyprotein;
DE   Contains:
DE     RecName: Full=Putative 2'-O-methyl transferase;
DE              EC=2.1.1.-;
DE     AltName: Full=nsp16;
DE   Flags: Fragment;
GN   Name=rep; ORFNames=1a-1b;
OS   Porcine respiratory coronavirus (strain 86/137004 / isolate British)
OS   (PRCoV) (PRCV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Tegacovirus.
OX   NCBI_TaxID=33736;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1662846; DOI=10.1016/0168-1702(91)90032-q;
RA   Britton P., Mawditt K.L., Page K.W.;
RT   "The cloning and sequencing of the virion protein genes from a British
RT   isolate of porcine respiratory coronavirus: comparison with transmissible
RT   gastroenteritis virus genes.";
RL   Virus Res. 21:181-198(1991).
CC   -!- FUNCTION: The replicase polyprotein of coronaviruses is a
CC       multifunctional protein: it contains the activities necessary for the
CC       transcription of negative stranded RNA, leader RNA, subgenomic mRNAs
CC       and progeny virion RNA as well as proteinases responsible for the
CC       cleavage of the polyprotein into functional products.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Ribosomal frameshifting; Named isoforms=2;
CC       Name=Replicase polyprotein 1ab; Synonyms=pp1ab;
CC         IsoId=Q04158-1; Sequence=Displayed;
CC       Name=Replicase polyprotein 1a; Synonyms=pp1a, ORF1a polyprotein;
CC         IsoId=Q04158-2; Sequence=Not described;
CC   -!- MISCELLANEOUS: [Isoform Replicase polyprotein 1ab]: Produced by -1
CC       ribosomal frameshifting at the 1a-1b genes boundary.
CC   -!- MISCELLANEOUS: [Isoform Replicase polyprotein 1a]: Produced by
CC       conventional translation. {ECO:0000305}.
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DR   EMBL; X60089; CAA42687.1; -; Genomic_RNA.
DR   PIR; S24285; S24285.
DR   SMR; Q04158; -.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR009461; NSP16_CoV-like.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF06460; CoV_Methyltr_2; 1.
DR   PROSITE; PS51955; NIV_2_O_MTASE; 1.
PE   4: Predicted;
KW   Methyltransferase; Ribosomal frameshifting; Transferase.
FT   CHAIN           <1..131
FT                   /note="Putative 2'-O-methyl transferase"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000037401"
FT   DOMAIN          <1..128
FT                   /note="Nidovirus-type SAM-dependent 2'-O-MTase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01300"
FT   NON_TER         1
SQ   SEQUENCE   131 AA;  15161 MW;  A3709FFF0A324D65 CRC64;
     ITEFSWNKYL YELIQRFEYW TVFCTSVNTS SSEGFLIGVN YLGPYCDKAI VDGNIMHANY
     IFWRNSTIMA LSHNSVLDTP KFKCRCNNAL IVNLKEKELN EMVIGLLRKG KLLIRNNGKL
     LNFGNHFINT P