ID   R1AB_CVTMI              Reviewed;         306 AA.
AC   Q9WQ76;
DT   10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   29-SEP-2021, entry version 72.
DE   RecName: Full=Replicase polyprotein 1ab;
DE            Short=pp1ab;
DE   AltName: Full=ORF1ab polyprotein;
DE   Contains:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              Short=Pol;
DE              Short=RdRp;
DE              EC=2.7.7.48;
DE     AltName: Full=nsp12;
DE   Flags: Fragment;
GN   Name=rep; ORFNames=1a-1b;
OS   Turkey coronavirus (strain Minnesota) (TCoV) (TCV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Gammacoronavirus; Igacovirus.
OX   NCBI_TaxID=231430;
OH   NCBI_TaxID=9103; Meleagris gallopavo (Wild turkey).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=10392726; DOI=10.1016/s0168-1702(99)00017-9;
RA   Stephensen C.B., Casebolt D.B., Gangopadhyay N.N.;
RT   "Phylogenetic analysis of a highly conserved region of the polymerase gene
RT   from 11 coronaviruses and development of a consensus polymerase chain
RT   reaction assay.";
RL   Virus Res. 60:181-189(1999).
CC   -!- FUNCTION: The replicase polyprotein of coronaviruses is a
CC       multifunctional protein: it contains the activities necessary for the
CC       transcription of negative stranded RNA, leader RNA, subgenomic mRNAs
CC       and progeny virion RNA as well as proteinases responsible for the
CC       cleavage of the polyprotein into functional products.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Ribosomal frameshifting; Named isoforms=2;
CC       Name=Replicase polyprotein 1ab; Synonyms=pp1ab;
CC         IsoId=Q9WQ76-1; Sequence=Displayed;
CC       Name=Replicase polyprotein 1a; Synonyms=pp1a, ORF1a polyprotein;
CC         IsoId=Q9WQ76-2; Sequence=Not described;
CC   -!- MISCELLANEOUS: [Isoform Replicase polyprotein 1ab]: Produced by -1
CC       ribosomal frameshifting at the 1a-1b genes boundary.
CC   -!- MISCELLANEOUS: [Isoform Replicase polyprotein 1a]: Produced by
CC       conventional translation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the coronaviruses polyprotein 1ab family.
CC       {ECO:0000305}.
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DR   EMBL; AF124991; AAD32995.1; -; Genomic_RNA.
DR   SMR; Q9WQ76; -.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR001205; RNA-dir_pol_C.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   Pfam; PF00680; RdRP_1; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS51948; COV_NSP12_RDRP; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Ribosomal frameshifting;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           <1..>306
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000037403"
FT   DOMAIN          <1..>306
FT                   /note="Nsp12 RNA-dependent RNA polymerase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01293"
FT   DOMAIN          106..268
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   NON_TER         1
FT   NON_TER         306
SQ   SEQUENCE   306 AA;  34874 MW;  52E573B1D84F13FE CRC64;
     FNKFGKARLY YEMSLEEQDQ LFESTKKNVL PTITQMNLKY AISAKNRART VAGVSILSTM
     TNRQFHQKIL KSIVNTRNAP VVIGTTKFYG GWDNMLRNLI QGVEDPILMG WDYPKCDRAM
     PNLLRITASL VLARKHTNCC TWSERIYRLY NECAQVLSET VLATGGIYVK PGGTSSGDAT
     TAYANSVFNI IQATSANVAR LLSVITRDIV YDDIKSLQYE LYQQVYRRVN FDPAFVEKFY
     SYLCKNFSLM IFADDGVVCY NNTLAKQGLV ADISGFREIL YYQNNVYMAD SKCWVEPDLE
     KGPHEF