R1A_LIYV9
ID R1A_LIYV9 Reviewed; 1873 AA.
AC Q83044;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Replicase polyprotein 1a;
DE Contains:
DE RecName: Full=Leader protease;
DE Short=L-PRO;
DE EC=3.4.22.-;
DE Contains:
DE RecName: Full=Methyltransferase/helicase;
DE EC=2.1.1.-;
DE EC=3.6.4.13;
GN ORFNames=ORF1a;
OS Lettuce infectious yellows virus (isolate United States/92) (LIYV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Closteroviridae; Crinivirus.
OX NCBI_TaxID=651355;
OH NCBI_TaxID=161934; Beta vulgaris (Sugar beet).
OH NCBI_TaxID=3654; Citrullus lanatus (Watermelon) (Citrullus vulgaris).
OH NCBI_TaxID=3656; Cucumis melo (Muskmelon).
OH NCBI_TaxID=3661; Cucurbita maxima (Pumpkin) (Winter squash).
OH NCBI_TaxID=3662; Cucurbita moschata (Winter crookneck squash) (Cucurbita pepo var. moschata).
OH NCBI_TaxID=3663; Cucurbita pepo (Vegetable marrow) (Summer squash).
OH NCBI_TaxID=4039; Daucus carota (Wild carrot).
OH NCBI_TaxID=4236; Lactuca sativa (Garden lettuce).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=11831736; DOI=10.1006/viro.1995.1133;
RA Klaassen V.A., Boeshore M.L., Koonin E.V., Tian T., Falk B.W.;
RT "Genome structure and phylogenetic analysis of lettuce infectious yellows
RT virus, a whitefly-transmitted, bipartite closterovirus.";
RL Virology 208:99-110(1995).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Ribosomal frameshifting; Named isoforms=2;
CC Comment=The replicase 1a is produced from conventional translation of
CC the 1a ORF. The replicase 1ab is generated probably by a +1 ribosomal
CC frameshifting mechanism occurring at the 1a-1b genes boundary.;
CC Name=Replicase 1a;
CC IsoId=Q83044-1; Sequence=Displayed;
CC Name=Replicase 1ab;
CC IsoId=Q83045-1; Sequence=External;
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DR EMBL; U15440; AAA61797.1; -; Genomic_RNA.
DR SMR; Q83044; -.
DR Proteomes; UP000001099; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
PE 4: Predicted;
KW ATP-binding; Helicase; Hydrolase; Methyltransferase; Nucleotide-binding;
KW Protease; Reference proteome; Ribosomal frameshifting; Thiol protease;
KW Transferase.
FT CHAIN 1..1873
FT /note="Replicase polyprotein 1a"
FT /id="PRO_0000402507"
FT CHAIN 1..413
FT /note="Leader protease"
FT /evidence="ECO:0000250"
FT /id="PRO_0000402508"
FT CHAIN 414..1873
FT /note="Methyltransferase/helicase"
FT /evidence="ECO:0000250"
FT /id="PRO_0000402509"
FT DOMAIN 495..674
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT DOMAIN 1538..1705
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 1706..1873
FT /note="(+)RNA virus helicase C-terminal"
FT REGION 1356..1381
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 344
FT /note="For leader protease activity"
FT /evidence="ECO:0000250"
FT ACT_SITE 393
FT /note="For leader protease activity"
FT /evidence="ECO:0000250"
FT SITE 413..414
FT /note="Cleavage; by the leader protease"
FT /evidence="ECO:0000250"
SQ SEQUENCE 1873 AA; 217266 MW; 55A771D8357761F0 CRC64;
MALEVESAFS LSYLLKENKQ TSNNLFSLIQ VPYTKMTHAK GKKTHLLRCI LGSPFETLTK
SSIRRSLKNL REVCNLPYCN FKKIPKNISY KYQNPFLKLY EINNNFINCK VGATGNTNST
YDWRNLSTIG NGWSVTSYHN DSVSRLHVSM VNNIPNSNTK KLSWWVTRVR SKNPWKRPYK
VIYQRDFRDD GIKPYVSEFI LYAPKNRADL EMQSILLSSV ECFQDIKYAL NYWTNLKFKE
ICNSALKRFT QTNIIITPVK PRVVSVNKAR EVSSSVEVKL QRYEREGEVF VVSKAGKEDT
RVLNDDNAIR NLFNATLNGG KYKLHPEAKS ATGKRFKYYK DGFCWLDVFA DANRRIPEWV
KPHCLLTGSV LMSCGLWDFA KRKMVSVSHG LLHYDRKLER SSARAGVRDF VGASNEAVQR
EDFRDLDLRV EEFAERVLET ANLRSDNRLI DNILTRASDY INKKSKESKE LDINVCLSMD
EKKMITNLFP DIQMSFNQKS YSNHGVFNAM RACENFYFSR KFKNSDYIDA GGDVVSTLRS
KNHNVHICSP RLDLKDAARH IQRATVIDGL KGYGETISFC TNKTEDCAVN RDIIIAVEVY
DMTLRDMAKA MLSHGSRKFE FSCIIPPEIF TKECNVELYE GRLKVTRIGD NVEYYYGSNG
ETFSHSCQTL KDILSVQVFQ FGGRVFKKTL EHSRGQLHFF SICICEKIEP GSVKLKTYYQ
RSELDKVTLR IPVKDSFGVV THYIIKEDRE FVSSMIEYVA NTGIKIDDKM VEWTYSQYRA
KKTVTIKSGK VTQKETRIRK ELIPGFIAII MSEGIRAREK THYLAKMLYT SHYKPSIVNI
IFRLIMHFLG GTKRFIYESL VDCLKFLTNS DYIDTIVNTE SRIEDLDKWF VFEQNVTITT
DAEDQPSILE QSVKTFLSKY SEDVYERGDR SEFELEEFSS EQDLVDQLLN SGGGSKEDEW
FYSYIFSIVR TKCTLSLAWR WTNRIFNFIT SCRAKGKDFV KYIYEIIKII INKPWEALCH
YLVQGYKTVL KIPMTCVDSV NYLKRKSLEK ILSYFSHERA DDEYIFPGYD SDFSEESGDY
GDENTELDSR SRCTVIKSKV EMLFRNIKGH IEKFLQRCGI IDQYNKIYHW FKSILTDVDC
FNSVLNFITH TGCDALLAVL TGHFSIASCA LKFMTDVVIE KNLSEKHHTT TKLIAGNLTQ
CIYKLDFIHP IWIPIRWGVK EISKIHIKKK LLNWRSTKEV TNESICKDLV HKSYIKYFDI
KKAVWLLWFS LLIIFLHPTL GFFILFSIYP AIEIKRYYNN VVCFNNIKMS YPHVIDRLGG
TYNFAKLKKA VREKFSENKS QGVKISEIPA EKDEDVMPPL EEVETPKRSV GRPSSKQDDI
EDECNIKEDP TESLNFSNIE ATSNRYDTLR VGNSKLCKFL RFYPKSKCYA NIQTGDLIRD
SIEEFYHLER SKLDIEISKM QRVVEIMNDT GRMLDNVRRM IDDRSMYVTL DGVSWYRLGC
KDKNPAKEDF KAIFDHNFNI MEGNAKVKEF AVSSDEWRGM YSNERCRAIE QLFNDNNEMV
RRPDVNGLKF YNKPPGAGKT TTIAKLMSKD LKNKVKCLAL SYTKVGRLEL IDKLKKDGIE
KPEKYVKTYD SFLMNNDNIL EIVNLYCDEV FMMHAGHFLT LLTKIAYQNG YCYGDVNQIP
FINRDPYTPA YLSREFFRKQ DLNYDTYTYR CPLDTCYLLS NLKDEMGNII YAGGVKNVNE
VYPTIRSLNL FGINVVGEVP VEYNAKYLTF TQDEKLNLQR HIDSQGGCRN AVSTVNEAQG
CTFSEVNLVR LVQFDNPVMS DINQFVVAIS RHTTTFKYFT PHSRLNDRVS NAISSLQSVS
DFVLKDYHFR QCL
