ID   R34K_CLOPA              Reviewed;         308 AA.
AC   P23160;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=34.2 kDa protein in rubredoxin operon;
DE            EC=1.8.1.-;
DE   AltName: Full=ORF A;
OS   Clostridium pasteurianum.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1501;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1637309; DOI=10.1042/bj2850255;
RA   Mathieu I., Meyer J., Moulis J.-M.;
RT   "Cloning, sequencing and expression in Escherichia coli of the rubredoxin
RT   gene from Clostridium pasteurianum.";
RL   Biochem. J. 285:255-262(1992).
CC   -!- SIMILARITY: Belongs to the class-II pyridine nucleotide-disulfide
CC       oxidoreductase family. {ECO:0000305}.
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DR   EMBL; M60116; AAA23276.1; -; Genomic_DNA.
DR   PIR; S29117; S29117.
DR   RefSeq; WP_003447690.1; NZ_CP013019.1.
DR   AlphaFoldDB; P23160; -.
DR   SMR; P23160; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0004791; F:thioredoxin-disulfide reductase activity; IEA:InterPro.
DR   GO; GO:0019430; P:removal of superoxide radicals; IEA:InterPro.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR008255; Pyr_nucl-diS_OxRdtase_2_AS.
DR   InterPro; IPR005982; Thioredox_Rdtase.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01292; TRX_reduct; 1.
DR   PROSITE; PS00573; PYRIDINE_REDOX_2; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; FAD; Flavoprotein; NAD; NADP; Oxidoreductase;
KW   Redox-active center.
FT   CHAIN           1..308
FT                   /note="34.2 kDa protein in rubredoxin operon"
FT                   /id="PRO_0000166790"
FT   BINDING         268..278
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
FT   DISULFID        136..139
FT                   /note="Redox-active"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   308 AA;  34215 MW;  52D9CF51208EFC37 CRC64;
     MKEEKQLDLV IIGAGPAGLT AAIYAIRAKL NTLVLENELV GGQIRETYTV ENFPGFNVIS
     GADLADKMEE HAASIGVNID QFSNIEKIKL SDDEKIIETE DVIYKVKALI IATGAKSRRL
     PIPEEEKLHG KVIHYCELCD GALYQGKDLV VVGGGNSAVE AAIFLTKYAR NITIVHQFDY
     LQAQKYSQDE LFKHKNVKII WDSEIRNIVG ENEIEKIVVE NVKTKQKTEL KADGVFVYIG
     YEPKTELFKD SININKWGYI ETDENMETNI KGVFAAGDVR SKLIRQLTTA VSDGTVAALM
     AEKYIGGK