R3GEF_MOUSE
ID R3GEF_MOUSE Reviewed; 383 AA.
AC Q8VDV3; Q3TTM0;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Guanine nucleotide exchange factor for Rab-3A;
DE AltName: Full=Rab-3A-interacting-like protein 1;
DE Short=Rab3A-interacting-like protein 1;
DE AltName: Full=Rabin3-like 1;
GN Name=Rab3il1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Kidney, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate
CC RAB3A, a GTPase that regulates synaptic vesicle exocytosis. Promotes
CC the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins
CC into their active GTP-bound form. May also activate RAB8A and RAB8B (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with RAB3A and IHPK1 through the coiled-coil domain.
CC This interaction is competitive. IHPK1 kinase activity is not required
CC for this interaction (By similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VDV3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VDV3-2; Sequence=VSP_028346;
CC -!- SIMILARITY: Belongs to the SEC2 family. {ECO:0000305}.
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DR EMBL; AK132764; BAE21345.1; -; mRNA.
DR EMBL; AK161298; BAE36305.1; -; mRNA.
DR EMBL; BC020147; AAH20147.1; -; mRNA.
DR CCDS; CCDS29569.1; -. [Q8VDV3-1]
DR CCDS; CCDS84417.1; -. [Q8VDV3-2]
DR RefSeq; NP_001334358.1; NM_001347429.1. [Q8VDV3-2]
DR RefSeq; NP_653121.1; NM_144538.2. [Q8VDV3-1]
DR AlphaFoldDB; Q8VDV3; -.
DR SMR; Q8VDV3; -.
DR BioGRID; 217000; 2.
DR STRING; 10090.ENSMUSP00000108786; -.
DR iPTMnet; Q8VDV3; -.
DR PhosphoSitePlus; Q8VDV3; -.
DR jPOST; Q8VDV3; -.
DR MaxQB; Q8VDV3; -.
DR PaxDb; Q8VDV3; -.
DR PeptideAtlas; Q8VDV3; -.
DR PRIDE; Q8VDV3; -.
DR ProteomicsDB; 301907; -. [Q8VDV3-1]
DR ProteomicsDB; 301908; -. [Q8VDV3-2]
DR Antibodypedia; 28364; 193 antibodies from 21 providers.
DR DNASU; 74760; -.
DR Ensembl; ENSMUST00000113161; ENSMUSP00000108786; ENSMUSG00000024663. [Q8VDV3-1]
DR Ensembl; ENSMUST00000121418; ENSMUSP00000113828; ENSMUSG00000024663. [Q8VDV3-2]
DR Ensembl; ENSMUST00000144788; ENSMUSP00000121449; ENSMUSG00000024663. [Q8VDV3-1]
DR GeneID; 74760; -.
DR KEGG; mmu:74760; -.
DR UCSC; uc008gov.1; mouse. [Q8VDV3-1]
DR CTD; 5866; -.
DR MGI; MGI:1922010; Rab3il1.
DR VEuPathDB; HostDB:ENSMUSG00000024663; -.
DR eggNOG; KOG4324; Eukaryota.
DR GeneTree; ENSGT00940000159102; -.
DR HOGENOM; CLU_038204_1_0_1; -.
DR InParanoid; Q8VDV3; -.
DR OrthoDB; 619794at2759; -.
DR PhylomeDB; Q8VDV3; -.
DR TreeFam; TF313748; -.
DR Reactome; R-MMU-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR BioGRID-ORCS; 74760; 5 hits in 71 CRISPR screens.
DR PRO; PR:Q8VDV3; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q8VDV3; protein.
DR Bgee; ENSMUSG00000024663; Expressed in seminiferous tubule of testis and 189 other tissues.
DR ExpressionAtlas; Q8VDV3; baseline and differential.
DR Genevisible; Q8VDV3; MM.
DR GO; GO:0070319; C:Golgi to plasma membrane transport vesicle; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0019900; F:kinase binding; ISO:MGI.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR040351; RAB3IL/RAB3IP/Sec2.
DR InterPro; IPR009449; Sec2_N.
DR PANTHER; PTHR14430; PTHR14430; 1.
DR Pfam; PF06428; Sec2p; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Guanine-nucleotide releasing factor;
KW Phosphoprotein; Protein transport; Reference proteome; Transport.
FT CHAIN 1..383
FT /note="Guanine nucleotide exchange factor for Rab-3A"
FT /id="PRO_0000305296"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 167..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 77..128
FT /evidence="ECO:0000255"
FT COMPBIAS 28..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 169
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 180
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TBN0"
FT VAR_SEQ 1..69
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028346"
SQ SEQUENCE 383 AA; 42713 MW; 30B2F1C12495BF82 CRC64;
MWSGQPQQDE GLPVGLSAIP VPWKNLGPSK SNRESSGGLV EASTSWEEAQ GEEHPAPAPL
DVSRLRSSSM EIREKGSEFL KEELYKAQKE LKLKDEECER LCKVRAQLEQ ELEELTASLF
EEAHKMVREA NMKQATSEKQ LKEAWGKIDM LQAEVTALKT LVITSTPASP NRELHPQLLS
PTKAGPRKGH SRQKSTSSSL CPVVCPTAGH IPTPDKEGKE VDTTLFAEFQ AWRASPTLDK
SCPFLERVYR EDVGPCLDFT VQELSALVRT AVEDNTLTIE PVASQTLPAV KVPTVECNNT
NTCALSGLAR TCHHRIRLGD SDSHYYISPS SRARITAVCN FFTYIRYIQQ GLVRQDAEPM
FWEIMRLRKG MSLAKLGFFP QEA
