R47BP_ARATH
ID R47BP_ARATH Reviewed; 425 AA.
AC Q8VXZ9; F4K135;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Polyadenylate-binding protein RBP47B';
DE Short=Poly(A)-binding protein RBP47B';
DE AltName: Full=RNA-binding protein 47B';
DE Short=AtRBP47B prime;
DE Short=AtRBP47B';
GN Name=RBP47B'; OrderedLocusNames=At5g19350; ORFNames=F7K24.100;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=21120628; DOI=10.1007/s10059-011-0001-2;
RA Peal L., Jambunathan N., Mahalingam R.;
RT "Phylogenetic and expression analysis of RNA-binding proteins with triple
RT RNA recognition motifs in plants.";
RL Mol. Cells 31:55-64(2011).
CC -!- FUNCTION: Heterogeneous nuclear ribonucleoprotein (hnRNP)-protein
CC binding the poly(A) tail of mRNA and probably involved in some steps of
CC pre-mRNA maturation. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with the poly(A) tail of mRNA in nucleus.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasmic granule
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VXZ9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VXZ9-2; Sequence=VSP_042355;
CC -!- SIMILARITY: Belongs to the polyadenylate-binding RBP47 family.
CC {ECO:0000305}.
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DR EMBL; AF296837; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92689.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92690.1; -; Genomic_DNA.
DR EMBL; AY074319; AAL67015.1; -; mRNA.
DR EMBL; AY114004; AAM45052.1; -; mRNA.
DR RefSeq; NP_001078604.1; NM_001085135.2. [Q8VXZ9-2]
DR RefSeq; NP_197436.1; NM_121940.5. [Q8VXZ9-1]
DR AlphaFoldDB; Q8VXZ9; -.
DR SMR; Q8VXZ9; -.
DR BioGRID; 17331; 1.
DR STRING; 3702.AT5G19350.1; -.
DR iPTMnet; Q8VXZ9; -.
DR PaxDb; Q8VXZ9; -.
DR PRIDE; Q8VXZ9; -.
DR ProteomicsDB; 236619; -. [Q8VXZ9-1]
DR EnsemblPlants; AT5G19350.1; AT5G19350.1; AT5G19350. [Q8VXZ9-1]
DR EnsemblPlants; AT5G19350.2; AT5G19350.2; AT5G19350. [Q8VXZ9-2]
DR GeneID; 832055; -.
DR Gramene; AT5G19350.1; AT5G19350.1; AT5G19350. [Q8VXZ9-1]
DR Gramene; AT5G19350.2; AT5G19350.2; AT5G19350. [Q8VXZ9-2]
DR KEGG; ath:AT5G19350; -.
DR Araport; AT5G19350; -.
DR TAIR; locus:2150215; AT5G19350.
DR eggNOG; KOG0118; Eukaryota.
DR OMA; YVKIPAN; -.
DR PhylomeDB; Q8VXZ9; -.
DR PRO; PR:Q8VXZ9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8VXZ9; baseline and differential.
DR Genevisible; Q8VXZ9; AT.
DR GO; GO:0010494; C:cytoplasmic stress granule; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005685; C:U1 snRNP; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0008143; F:poly(A) binding; ISS:UniProtKB.
DR GO; GO:0034605; P:cellular response to heat; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:TAIR.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 3.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; mRNA processing; Nucleus; Reference proteome; Repeat;
KW RNA-binding.
FT CHAIN 1..425
FT /note="Polyadenylate-binding protein RBP47B'"
FT /id="PRO_0000415768"
FT DOMAIN 24..102
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 116..195
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 237..309
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT VAR_SEQ 209..212
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_042355"
SQ SEQUENCE 425 AA; 46597 MW; 34D888E44BB1F89A CRC64;
MAMMHPPQPP QGSYHHPQTL EEVRTLWIGD LQYWVDENYL TSCFSQTGEL VSVKVIRNKI
TGQPEGYGFI EFISHAAAER TLQTYNGTQM PGTELTFRLN WASFGSGQKV DAGPDHSIFV
GDLAPDVTDY LLQETFRVHY SSVRGAKVVT DPSTGRSKGY GFVKFAEESE RNRAMAEMNG
LYCSTRPMRI SAATPKKNVG VQQQYVTKAV YPVTVPSAVA APVQAYVAPP ESDVTCTTIS
VANLDQNVTE EELKKAFSQL GEVIYVKIPA TKGYGYVQFK TRPSAEEAVQ RMQGQVIGQQ
AVRISWSKNP GQDGWVTQAD PNQWNGYYGY GQGYDAYAYG ATQDPSVYAY GGYGYPQYPQ
QGEGTQDISN SAAGGVAGAE QELYDPLATP DVDKLNAAYL SVHASAILGR PMWQRTSSLT
SQLGK
