R51A1_MAIZE
ID R51A1_MAIZE Reviewed; 340 AA.
AC Q67EU8; Q9XED6;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=DNA repair protein RAD51 homolog A;
DE AltName: Full=Rad51-like protein A;
DE Short=RAD51A;
DE AltName: Full=ZmRAD51a;
GN Name=RAD51A;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. A632;
RX PubMed=10330467; DOI=10.2307/3870816;
RA Franklin A.E., McElver J., Sunjevaric I., Rothstein R., Bowen B.,
RA Cande W.Z.;
RT "Three-dimensional microscopy of the Rad51 recombination protein during
RT meiotic prophase.";
RL Plant Cell 11:809-824(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-198.
RC STRAIN=cv. B73;
RA Li J., Weber D., Meeley R.B., Bowen B., Schnable P.S.;
RT "Maize RAD51 is required for chromosome segregation but not for chromosome
RT pairing, meiotic recombination or somatic cell viability.";
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RX PubMed=12897254; DOI=10.1105/tpc.012898;
RA Pawlowski W.P., Golubovskaya I.N., Cande W.Z.;
RT "Altered nuclear distribution of recombination protein RAD51 in maize
RT mutants suggests the involvement of RAD51 in meiotic homology
RT recognition.";
RL Plant Cell 15:1807-1816(2003).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=12811575; DOI=10.1007/s00412-003-0242-8;
RA Franklin A.E., Golubovskaya I.N., Bass H.W., Cande W.Z.;
RT "Improper chromosome synapsis is associated with elongated RAD51 structures
RT in the maize desynaptic2 mutant.";
RL Chromosoma 112:17-25(2003).
CC -!- FUNCTION: Binds to single and double-stranded DNA and exhibits DNA-
CC dependent ATPase activity. Unwinds duplex DNA (By similarity).
CC Component of the meiotic recombination pathway. Seems to play a role in
CC mediating chromosome homology search, chromosome pairing and synapsis
CC at early stages and probably chromosome crossing-over at later stages
CC in meiosis. Probably is involved in the repair of meiotic double strand
CC breaks (DBSs) and in homologous recombination. {ECO:0000250,
CC ECO:0000269|PubMed:10330467, ECO:0000269|PubMed:12897254}.
CC -!- SUBUNIT: Self-associates and may interact with XRCC3 homolog.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12811575}.
CC -!- TISSUE SPECIFICITY: Highly expressed in mitotic and meiotic tissues,
CC but low levels in differentiated tissues.
CC {ECO:0000269|PubMed:10330467}.
CC -!- SIMILARITY: Belongs to the RecA family. RAD51 subfamily. {ECO:0000305}.
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DR EMBL; AF079428; AAD32029.1; -; mRNA.
DR EMBL; AY359681; AAR08149.1; -; Genomic_DNA.
DR RefSeq; NP_001104918.1; NM_001111448.1.
DR AlphaFoldDB; Q67EU8; -.
DR SMR; Q67EU8; -.
DR STRING; 4577.GRMZM2G121543_P02; -.
DR PaxDb; Q67EU8; -.
DR GeneID; 541709; -.
DR KEGG; zma:541709; -.
DR MaizeGDB; 112974; -.
DR eggNOG; KOG1433; Eukaryota.
DR OrthoDB; 877394at2759; -.
DR Proteomes; UP000007305; Unplaced.
DR ExpressionAtlas; Q67EU8; baseline and differential.
DR GO; GO:0000794; C:condensed nuclear chromosome; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008094; F:ATP-dependent activity, acting on DNA; IBA:GO_Central.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0000150; F:DNA strand exchange activity; IBA:GO_Central.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0070192; P:chromosome organization involved in meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0000730; P:DNA recombinase assembly; IBA:GO_Central.
DR GO; GO:0006312; P:mitotic recombination; IBA:GO_Central.
DR GO; GO:1990426; P:mitotic recombination-dependent replication fork processing; IEA:InterPro.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IBA:GO_Central.
DR GO; GO:0042148; P:strand invasion; IBA:GO_Central.
DR CDD; cd01123; Rad51_DMC1_radA; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011941; DNA_recomb/repair_Rad51.
DR InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR InterPro; IPR016467; DNA_recomb/repair_RecA-like.
DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR033925; Rad51_DMC1_RadA.
DR InterPro; IPR020588; RecA_ATP-bd.
DR InterPro; IPR020587; RecA_monomer-monomer_interface.
DR Pfam; PF08423; Rad51; 1.
DR PIRSF; PIRSF005856; Rad51; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF47794; SSF47794; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02239; recomb_RAD51; 1.
DR PROSITE; PS50162; RECA_2; 1.
DR PROSITE; PS50163; RECA_3; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..340
FT /note="DNA repair protein RAD51 homolog A"
FT /id="PRO_0000122928"
FT DOMAIN 49..78
FT /note="HhH"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 128..135
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 141
FT /note="P -> L (in Ref. 2; AAR08149)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 340 AA; 36705 MW; 08CE812EC558A2F4 CRC64;
MSSAAQQQQK AAAAEQEEVE HGPFPIEQLQ ASGIAALDVK KLKDSGLHTV EAVAYTPRKD
LLQIKGISEA KADKIIEAAS KIVPLGFTSA SQLHAQRLEI IQVTTGSREL DKILEGGIET
GSITEIYGEF RSGKTQLCHT PCVTCQLPLD QGGGEGKALY IDAEGTFRPQ RLLQIADRFG
LNGADVLENV AYARAYNTDH QSRLLLEAAS MMIETRFALM VVDSATALYR TDFSGRGELS
ARQMHMAKFL RSLQKLADEF GVAVVITNQV VAQVDGSAMF AGPQFKPIGG NIMAHASTTR
LALRKGRGEE RICKVISSPC LAEAEARFQL ASEGIADVKD
