R9BP_MOUSE
ID R9BP_MOUSE Reviewed; 237 AA.
AC Q148R9; Q8K462;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Regulator of G-protein signaling 9-binding protein;
DE AltName: Full=RGS9-anchoring protein;
GN Name=Rgs9bp; Synonyms=R9ap;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP INTERACTION WITH RGS9 AND GNB5.
RC STRAIN=C57BL/6 X 129; TISSUE=Retina;
RX PubMed=12119397; DOI=10.1073/pnas.152094799;
RA Hu G., Wensel T.G.;
RT "R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein,
RT RGS9-1.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:9755-9760(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Retina;
RX PubMed=14664818; DOI=10.1016/s1044-7431(03)00231-8;
RA Keresztes G., Mutai H., Hibino H., Hudspeth A.J., Heller S.;
RT "Expression patterns of the RGS9-1 anchoring protein R9AP in the chicken
RT and mouse suggest multiple roles in the nervous system.";
RL Mol. Cell. Neurosci. 24:687-695(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH RGS9.
RX PubMed=12499365; DOI=10.1074/jbc.m211782200;
RA Sokal I., Hu G., Liang Y., Mao M., Wensel T.G., Palczewski K.;
RT "Identification of protein kinase C isozymes responsible for the
RT phosphorylation of photoreceptor-specific RGS9-1 at Ser475.";
RL J. Biol. Chem. 278:8316-8325(2003).
RN [5]
RP FUNCTION.
RX PubMed=12560335; DOI=10.1074/jbc.m212046200;
RA Hu G., Zhang Z., Wensel T.G.;
RT "Activation of RGS9-1GTPase acceleration by its membrane anchor, R9AP.";
RL J. Biol. Chem. 278:14550-14554(2003).
RN [6]
RP INTERACTION WITH RGS9.
RX PubMed=14614075; DOI=10.1523/jneurosci.23-32-10175.2003;
RA Martemyanov K.A., Lishko P.V., Calero N., Keresztes G., Sokolov M.,
RA Strissel K.J., Leskov I.B., Hopp J.A., Kolesnikov A.V., Chen C.-K., Lem J.,
RA Heller S., Burns M.E., Arshavsky V.Y.;
RT "The DEP domain determines subcellular targeting of the GTPase activating
RT protein RGS9 in vivo.";
RL J. Neurosci. 23:10175-10181(2003).
RN [7]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=14625292; DOI=10.1074/jbc.c300456200;
RA Keresztes G., Martemyanov K.A., Krispel C.M., Mutai H., Yoo P.J.,
RA Maison S.F., Burns M.E., Arshavsky V.Y., Heller S.;
RT "Absence of the RGS9.Gbeta5 GTPase-activating complex in photoreceptors of
RT the R9AP knockout mouse.";
RL J. Biol. Chem. 279:1581-1584(2004).
RN [8]
RP FUNCTION.
RX PubMed=16939221; DOI=10.1021/bi060376a;
RA Baker S.A., Martemyanov K.A., Shavkunov A.S., Arshavsky V.Y.;
RT "Kinetic mechanism of RGS9-1 potentiation by R9AP.";
RL Biochemistry 45:10690-10697(2006).
RN [9]
RP FUNCTION, AND INTERACTION WITH RGS9 AND GNB5.
RX PubMed=16908407; DOI=10.1016/j.neuron.2006.07.010;
RA Krispel C.M., Chen D., Melling N., Chen Y.-J., Martemyanov K.A.,
RA Quillinan N., Arshavsky V.Y., Wensel T.G., Chen C.-K., Burns M.E.;
RT "RGS expression rate-limits recovery of rod photoresponses.";
RL Neuron 51:409-416(2006).
CC -!- FUNCTION: Regulator of G protein-coupled receptor (GPCR) signaling in
CC phototransduction. Participates in the recovery phase of visual
CC transduction via its interaction with RGS9-1 isoform. Acts as a
CC membrane-anchor that mediates the targeting of RGS9-1 to the
CC photoreceptor outer segment, where phototransduction takes place.
CC Enhances the ability of RGS9-1 to stimulate G protein GTPase activity,
CC allowing the visual signal to be terminated on the physiologically time
CC scale. It also controls the proteolytic stability of RGS9-1, probably
CC by protecting it from degradation. {ECO:0000269|PubMed:12560335,
CC ECO:0000269|PubMed:14625292, ECO:0000269|PubMed:16908407,
CC ECO:0000269|PubMed:16939221}.
CC -!- SUBUNIT: Specifically interacts with isoform RGS9-1 of RGS9.
CC Interaction is decreased when RGS9-1 is phosphorylated at 'Ser-475'.
CC Component of the RGS9-1-Gbeta5 complex composed of RGS9-1, Gbeta5
CC (GNB5) and RGS9BP. {ECO:0000269|PubMed:12119397,
CC ECO:0000269|PubMed:12499365, ECO:0000269|PubMed:14614075,
CC ECO:0000269|PubMed:16908407}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:12119397}; Single-
CC pass type IV membrane protein {ECO:0000269|PubMed:12119397}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in photoreceptors of the
CC retina. Weakly expressed in other areas of the central nervous system.
CC {ECO:0000269|PubMed:12119397, ECO:0000269|PubMed:14664818}.
CC -!- DISRUPTION PHENOTYPE: Mice show light responses that recover at a
CC abnormally slow rate. {ECO:0000269|PubMed:14625292}.
CC -!- SIMILARITY: Belongs to the RGS7BP/RGS9BP family. {ECO:0000305}.
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DR EMBL; AF483907; AAM76160.1; -; mRNA.
DR EMBL; AY229888; AAP55847.1; -; mRNA.
DR EMBL; BC118006; AAI18007.1; -; mRNA.
DR CCDS; CCDS21153.1; -.
DR RefSeq; NP_665839.1; NM_145840.3.
DR AlphaFoldDB; Q148R9; -.
DR SMR; Q148R9; -.
DR STRING; 10090.ENSMUSP00000065511; -.
DR PhosphoSitePlus; Q148R9; -.
DR PaxDb; Q148R9; -.
DR PRIDE; Q148R9; -.
DR ProteomicsDB; 300370; -.
DR Antibodypedia; 55122; 25 antibodies from 9 providers.
DR DNASU; 243923; -.
DR Ensembl; ENSMUST00000069912; ENSMUSP00000065511; ENSMUSG00000056043.
DR GeneID; 243923; -.
DR KEGG; mmu:243923; -.
DR UCSC; uc009gkb.2; mouse.
DR CTD; 388531; -.
DR MGI; MGI:2384418; Rgs9bp.
DR VEuPathDB; HostDB:ENSMUSG00000056043; -.
DR eggNOG; ENOG502QT8D; Eukaryota.
DR GeneTree; ENSGT00940000153725; -.
DR HOGENOM; CLU_093021_0_0_1; -.
DR InParanoid; Q148R9; -.
DR OMA; MINEMEM; -.
DR OrthoDB; 1602757at2759; -.
DR PhylomeDB; Q148R9; -.
DR TreeFam; TF331562; -.
DR Reactome; R-MMU-2514859; Inactivation, recovery and regulation of the phototransduction cascade.
DR BioGRID-ORCS; 243923; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Rgs9bp; mouse.
DR PRO; PR:Q148R9; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q148R9; protein.
DR Bgee; ENSMUSG00000056043; Expressed in retinal neural layer and 38 other tissues.
DR Genevisible; Q148R9; MM.
DR GO; GO:0016021; C:integral component of membrane; TAS:MGI.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0001750; C:photoreceptor outer segment; IBA:GO_Central.
DR GO; GO:0050908; P:detection of light stimulus involved in visual perception; IMP:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0009968; P:negative regulation of signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR026512; RGS7BP/RGS9BP.
DR InterPro; IPR026513; RGS9BP.
DR PANTHER; PTHR21029; PTHR21029; 1.
DR PANTHER; PTHR21029:SF1; PTHR21029:SF1; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Membrane; Reference proteome; Sensory transduction;
KW Signal transduction inhibitor; Transmembrane; Transmembrane helix; Vision.
FT CHAIN 1..237
FT /note="Regulator of G-protein signaling 9-binding protein"
FT /id="PRO_0000287587"
FT TOPO_DOM 1..212
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..237
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 153..202
FT /note="SNARE-like"
FT COILED 30..54
FT /evidence="ECO:0000255"
FT CONFLICT 182
FT /note="G -> D (in Ref. 3; AAI18007)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 237 AA; 25177 MW; EFDE190A142FF24F CRC64;
MAREECKALL DALNKTTACY HHLVLTVGGS ADTQDLREEL QKTRQKAREL AVATGARLTV
ALRDRSLATE ERAEFERLWV AFSGCLDLLE ADMQRALALG ATFPLHAPRR PLVRTGVTGG
SSAVAARALS ARSLRHEAES DFDVADLPQL EREVLQVGEM IDDMEMKVNV PRWTVQARQA
AGAELLSGAS AGASSAGGIS VEERAGPCDP SKALAATVFS AVLLVAVALA LCVAKLS
