RA05_ORYSJ
ID RA05_ORYSJ Reviewed; 160 AA.
AC Q01881; Q40655; Q8GTK5;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 2.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Seed allergenic protein RA5;
DE AltName: Allergen=Ory s aA_TI;
DE Flags: Precursor;
GN Name=RA5; OrderedLocusNames=Os07g0215500, LOC_Os07g11510;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Seed;
RX PubMed=7678765; DOI=10.1007/bf00019940;
RA Adachi T., Izumi H., Yamada T., Tanaka K., Takeuchi S., Nakamura R.,
RA Matsuda T.;
RT "Gene structure and expression of rice seed allergenic proteins belonging
RT to the alpha-amylase/trypsin inhibitor family.";
RL Plant Mol. Biol. 21:239-248(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Seed endosperm;
RX PubMed=7669811; DOI=10.1016/0167-4838(95)00075-6;
RA Alvarez A.M., Adachi T., Nakase M., Aoki N., Nakamura R., Matsuda T.;
RT "Classification of rice allergenic protein cDNAs belonging to the alpha-
RT amylase/trypsin inhibitor gene family.";
RL Biochim. Biophys. Acta 1251:201-204(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND ALLERGEN.
RX PubMed=24016103; DOI=10.1021/jf402759f;
RA Golias J., Humlova Z., Halada P., Habova V., Janatkova I., Tuckova L.;
RT "Identification of rice proteins recognized by the IgE antibodies of
RT patients with food allergies.";
RL J. Agric. Food Chem. 61:8851-8860(2013).
CC -!- FUNCTION: Seed storage protein. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- PTM: Five disulfide bonds are present. {ECO:0000250|UniProtKB:Q01883}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE.
CC {ECO:0000269|PubMed:24016103}.
CC -!- SIMILARITY: Belongs to the protease inhibitor I6 (cereal trypsin/alpha-
CC amylase inhibitor) family. {ECO:0000305}.
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DR EMBL; D11430; BAA01996.1; -; mRNA.
DR EMBL; D42142; BAA07713.1; -; mRNA.
DR EMBL; AP003963; BAC19997.1; -; Genomic_DNA.
DR EMBL; AP014963; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S31078; S31078.
DR PIR; S59925; S59925.
DR RefSeq; XP_015644725.1; XM_015789239.1.
DR AlphaFoldDB; Q01881; -.
DR SMR; Q01881; -.
DR STRING; 4530.OS07T0215500-01; -.
DR Allergome; 1049; Ory s aA_TI.
DR PaxDb; Q01881; -.
DR GeneID; 4342727; -.
DR KEGG; osa:4342727; -.
DR eggNOG; ENOG502R43K; Eukaryota.
DR InParanoid; Q01881; -.
DR OrthoDB; 1724634at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR Genevisible; Q01881; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019863; F:IgE binding; IDA:UniProtKB.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; ISS:Gramene.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR002411; Allergen/amylase_inhib_rice.
DR InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PIRSF; PIRSF001657; Allergen/amylase_inhib; 1.
DR PRINTS; PR00808; AMLASEINHBTR.
DR PRINTS; PR00809; RAGALLERGEN.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
DR PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
PE 1: Evidence at protein level;
KW Allergen; Disulfide bond; Reference proteome; Secreted;
KW Seed storage protein; Signal; Storage protein.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..160
FT /note="Seed allergenic protein RA5"
FT /id="PRO_0000014359"
FT DISULFID 42..92
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 56..80
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 64..124
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 81..140
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 94..152
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT CONFLICT 38..41
FT /note="PAPL -> RAR (in Ref. 1; BAA01996)"
FT /evidence="ECO:0000305"
FT CONFLICT 52
FT /note="P -> S (in Ref. 1; BAA01996)"
FT /evidence="ECO:0000305"
FT CONFLICT 65..66
FT /note="Missing (in Ref. 1; BAA01996)"
FT /evidence="ECO:0000305"
FT CONFLICT 69
FT /note="T -> S (in Ref. 1; BAA01996)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 160 AA; 17293 MW; 506A22E066723585 CRC64;
MASNKVVFSV LLLAVVSVLA ATATMAEYHH QDQVVYTPAP LCQPGMGYPM YPLPRCRALV
KRQCVGRGTA AAAEQVRRDC CRQLAAVDDS WCRCEAISHM LGGIYRELGA PDVGHPMSEV
FRGCRRGDLE RAAASLPAFC NVDIPNGGGG VCYWLARSGY
