RA16_ORYSJ
ID RA16_ORYSJ Reviewed; 157 AA.
AC Q8H4L8; Q40654;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Alpha-amylase/trypsin inhibitor RA16 {ECO:0000305};
DE AltName: Full=Allergen RA16 {ECO:0000305};
DE AltName: Allergen=Ory s aA_TI;
DE Flags: Precursor;
GN Name=RA16 {ECO:0000305};
GN OrderedLocusNames=Os07g0214600 {ECO:0000312|EMBL:BAF21100.1};
GN ORFNames=OJ1116_C08.118 {ECO:0000312|EMBL:BAC20657.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Endosperm;
RX PubMed=7669811; DOI=10.1016/0167-4838(95)00075-6;
RA Alvarez A.M., Adachi T., Nakase M., Aoki N., Nakamura R., Matsuda T.;
RT "Classification of rice allergenic protein cDNAs belonging to the alpha-
RT amylase/trypsin inhibitor gene family.";
RL Biochim. Biophys. Acta 1251:201-204(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND ALLERGEN.
RX PubMed=23763241; DOI=10.1021/pr4002146;
RA Kurokawa S., Nakamura R., Mejima M., Kozuka-Hata H., Kuroda M.,
RA Takeyama N., Oyama M., Satoh S., Kiyono H., Masumura T., Teshima R.,
RA Yuki Y.;
RT "MucoRice-cholera toxin B-subunit, a rice-based oral cholera vaccine, down-
RT regulates the expression of alpha-amylase/trypsin inhibitor-like protein
RT family as major rice allergens.";
RL J. Proteome Res. 12:3372-3382(2013).
CC -!- FUNCTION: Seed storage protein. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- PTM: Five disulfide bonds are present. {ECO:0000250|UniProtKB:Q01883}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE.
CC {ECO:0000269|PubMed:23763241}.
CC -!- SIMILARITY: Belongs to the cereal trypsin/alpha-amylase inhibitor
CC family. {ECO:0000305}.
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DR EMBL; D42141; BAA07712.1; -; mRNA.
DR EMBL; AP004002; BAC20657.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21100.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT00620.1; -; Genomic_DNA.
DR PIR; S59924; S59924.
DR RefSeq; XP_015645223.1; XM_015789737.1.
DR AlphaFoldDB; Q8H4L8; -.
DR SMR; Q8H4L8; -.
DR STRING; 4530.OS07T0214600-01; -.
DR Allergome; 1049; Ory s aA_TI.
DR PaxDb; Q8H4L8; -.
DR PRIDE; Q8H4L8; -.
DR EnsemblPlants; Os07t0214600-01; Os07t0214600-01; Os07g0214600.
DR GeneID; 4342724; -.
DR Gramene; Os07t0214600-01; Os07t0214600-01; Os07g0214600.
DR KEGG; osa:4342724; -.
DR eggNOG; ENOG502R43K; Eukaryota.
DR HOGENOM; CLU_140628_0_0_1; -.
DR InParanoid; Q8H4L8; -.
DR OMA; NSGPWMW; -.
DR OrthoDB; 1466684at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019863; F:IgE binding; IDA:UniProtKB.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:InterPro.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR002411; Allergen/amylase_inhib_rice.
DR InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PIRSF; PIRSF001657; Allergen/amylase_inhib; 1.
DR PRINTS; PR00808; AMLASEINHBTR.
DR PRINTS; PR00809; RAGALLERGEN.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
DR PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
PE 1: Evidence at protein level;
KW Allergen; Disulfide bond; Reference proteome; Secreted;
KW Seed storage protein; Signal; Storage protein.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..157
FT /note="Alpha-amylase/trypsin inhibitor RA16"
FT /id="PRO_5007214320"
FT DISULFID 41..89
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 55..77
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 63..121
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 78..137
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 91..149
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT CONFLICT 9
FT /note="S -> P (in Ref. 1; BAA07712)"
FT /evidence="ECO:0000305"
FT CONFLICT 69
FT /note="V -> L (in Ref. 1; BAA07712)"
FT /evidence="ECO:0000305"
FT CONFLICT 83
FT /note="A -> G (in Ref. 1; BAA07712)"
FT /evidence="ECO:0000305"
FT CONFLICT 105..106
FT /note="SG -> ER (in Ref. 1; BAA07712)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 157 AA; 16983 MW; E0C753F320BA31FA CRC64;
MASNKVVISA LLVVVVSVLA ATTTMADHHQ EQVVYTPGQL CQPGIGYPTY PLPRCRAFVK
RQCVAPGTVD EQVRRGCCRQ LAAIDSSWCR CDALNHMLRI IYRESGAADA GHPMAEVFRG
CRRGDIERAA ASLPAFCNVD IPNGVGGVCY WLPGTGY
