ID   RA51A_XENLA             Reviewed;         336 AA.
AC   Q91918; Q5HZQ1;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=DNA repair protein RAD51 homolog A;
DE            Short=xRAD51.1;
GN   Name=rad51-a; Synonyms=rad51-1, rad51.1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Ovary;
RX   PubMed=7642095; DOI=10.1016/0378-1119(95)00148-y;
RA   Maeshima K., Morimatsu K., Shinohara A., Horii T.;
RT   "RAD51 homologues in Xenopus laevis: two distinct genes are highly
RT   expressed in ovary and testis.";
RL   Gene 160:195-200(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Egg;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays an important role in homologous strand exchange, a key
CC       step in DNA repair through homologous recombination (HR). Binds to
CC       single-stranded DNA in an ATP-dependent manner to form nucleoprotein
CC       filaments which are essential for the homology search and strand
CC       exchange. Catalyzes the recognition of homology and strand exchange
CC       between homologous DNA partners to form a joint molecule between a
CC       processed DNA break and the repair template. Recruited to resolve
CC       stalled replication forks during replication stress. Also involved in
CC       interstrand cross-link repair. {ECO:0000250|UniProtKB:Q06609}.
CC   -!- SUBUNIT: Forms linear homooligomers, giving rise to a RAD51
CC       nucleoprotein filament, which is essential for strand-pairing reactions
CC       during DNA recombination. {ECO:0000250|UniProtKB:Q06609}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q06609}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q06609}. Chromosome
CC       {ECO:0000250|UniProtKB:Q06609}. Note=Accumulated at sites of DNA
CC       damage. Recruited to stalled replication forks during replication
CC       stress. {ECO:0000250|UniProtKB:Q06609}.
CC   -!- SIMILARITY: Belongs to the RecA family. RAD51 subfamily. {ECO:0000305}.
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DR   EMBL; D38489; BAA07501.1; -; mRNA.
DR   EMBL; BC088930; AAH88930.1; -; mRNA.
DR   RefSeq; NP_001081236.1; NM_001087767.1.
DR   AlphaFoldDB; Q91918; -.
DR   SMR; Q91918; -.
DR   DNASU; 397726; -.
DR   GeneID; 397726; -.
DR   KEGG; xla:397726; -.
DR   CTD; 397726; -.
DR   Xenbase; XB-GENE-1016887; rad51.S.
DR   OrthoDB; 877394at2759; -.
DR   Proteomes; UP000186698; Chromosome 8S.
DR   Bgee; 397726; Expressed in oocyte and 11 other tissues.
DR   GO; GO:0005694; C:chromosome; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0000228; C:nuclear chromosome; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0000150; F:DNA strand exchange activity; IEA:InterPro.
DR   GO; GO:0003690; F:double-stranded DNA binding; ISS:UniProtKB.
DR   GO; GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
DR   GO; GO:0017116; F:single-stranded DNA helicase activity; ISS:UniProtKB.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR   GO; GO:0000730; P:DNA recombinase assembly; ISS:UniProtKB.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; ISS:UniProtKB.
DR   GO; GO:1990426; P:mitotic recombination-dependent replication fork processing; IEA:InterPro.
DR   CDD; cd01123; Rad51_DMC1_radA; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR011941; DNA_recomb/repair_Rad51.
DR   InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR   InterPro; IPR016467; DNA_recomb/repair_RecA-like.
DR   InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR033925; Rad51_DMC1_RadA.
DR   InterPro; IPR020588; RecA_ATP-bd.
DR   InterPro; IPR020587; RecA_monomer-monomer_interface.
DR   Pfam; PF08423; Rad51; 1.
DR   PIRSF; PIRSF005856; Rad51; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF47794; SSF47794; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02239; recomb_RAD51; 1.
DR   PROSITE; PS50162; RECA_2; 1.
DR   PROSITE; PS50163; RECA_3; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Chromosome; Cytoplasm; DNA-binding; Nucleotide-binding;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..336
FT                   /note="DNA repair protein RAD51 homolog A"
FT                   /id="PRO_0000122936"
FT   DOMAIN          45..74
FT                   /note="HhH"
FT   MOTIF           242..257
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250|UniProtKB:Q06609"
FT   BINDING         124..131
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   336 AA;  36661 MW;  A2620DF8C580D2BA CRC64;
     MAMQAHYEAE ATEEEHFGPQ AISRLEQCGI NANDVKKLEE AGFHTVEAVA YAPKKELLNI
     KGISEAKAEK ILAEAAKLVP MGFTTATEFH QRRSEIIQIS TGSKELDKLL QGGVETGSIT
     EMFGEFRTGK TQLCHTLAVT CQLPIDRGGG EGKAMYIDTE GTFRPERLLA VAERYGLSGS
     DVLDNVAYAR AFNTDHQTQL LYQASAMMAE SRYALLIVDS ATALYRTDYS GRGELSARQM
     HLARFLRMLL RLADEFGVAV VITNQVVAQV DGAAMFAADP KKPIGGNIIA HASTTRLYLR
     KGRGETRICK IYDSPCLPEA EAMFAINADG VGDAKD