RA51B_ARATH
ID RA51B_ARATH Reviewed; 370 AA.
AC Q9SK02; Q5H793; Q5H794; Q5K4Q0;
DT 09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=DNA repair protein RAD51 homolog 2;
DE Short=AtRAD51B;
GN Name=RAD51B; OrderedLocusNames=At2g28560; ORFNames=T17D12.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RA Siaud N.;
RT "A study of RAD51 homologues from Arabidopsis.";
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, AND
RP INDUCTION.
RC STRAIN=cv. Columbia; TISSUE=Flower bud;
RX PubMed=15952068; DOI=10.1007/s11103-005-2187-1;
RA Osakabe K., Abe K., Yamanouchi H., Takyuu T., Yoshioka T., Ito Y., Kato T.,
RA Tabata S., Kurei S., Yoshioka Y., Machida Y., Seki M., Kobayashi M.,
RA Shinozaki K., Ichikawa H., Toki S.;
RT "Arabidopsis Rad51B is important for double-strand DNA breaks repair in
RT somatic cells.";
RL Plant Mol. Biol. 57:819-833(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: May be involved in the homologous recombination repair (HRR)
CC pathway of double-stranded DNA breaks arising during DNA replication or
CC induced by DNA-damaging agents. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=RAD51Balpha;
CC IsoId=Q9SK02-1; Sequence=Displayed;
CC Name=2; Synonyms=RAD51Bbeta;
CC IsoId=Q9SK02-2; Sequence=VSP_016462, VSP_016463, VSP_016464;
CC Name=3; Synonyms=RAD51Bbeta;
CC IsoId=Q9SK02-3; Sequence=VSP_016465;
CC -!- TISSUE SPECIFICITY: Preferentially expressed in flower buds and roots.
CC {ECO:0000269|PubMed:15952068}.
CC -!- INDUCTION: Induced by ionizing irradiation and treatment with a cross-
CC linking reagent, cisplatin. {ECO:0000269|PubMed:15952068}.
CC -!- MISCELLANEOUS: [Isoform 3]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the RecA family. RAD51 subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD21490.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAD70704.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AJ549435; CAD70704.1; ALT_FRAME; mRNA.
DR EMBL; AB194809; BAD89163.1; -; mRNA.
DR EMBL; AB194810; BAD89164.1; -; mRNA.
DR EMBL; AC006587; AAD21490.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08140.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08141.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08143.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61239.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61240.1; -; Genomic_DNA.
DR PIR; D84686; D84686.
DR RefSeq; NP_001031438.1; NM_001036361.2. [Q9SK02-1]
DR RefSeq; NP_001154538.1; NM_001161066.1. [Q9SK02-2]
DR RefSeq; NP_001323469.1; NM_001336173.1. [Q9SK02-1]
DR RefSeq; NP_001323470.1; NM_001336172.1. [Q9SK02-1]
DR RefSeq; NP_180423.3; NM_128416.4. [Q9SK02-3]
DR AlphaFoldDB; Q9SK02; -.
DR SMR; Q9SK02; -.
DR BioGRID; 2754; 1.
DR STRING; 3702.AT2G28560.1; -.
DR PaxDb; Q9SK02; -.
DR PRIDE; Q9SK02; -.
DR EnsemblPlants; AT2G28560.1; AT2G28560.1; AT2G28560. [Q9SK02-3]
DR EnsemblPlants; AT2G28560.2; AT2G28560.2; AT2G28560. [Q9SK02-1]
DR EnsemblPlants; AT2G28560.4; AT2G28560.4; AT2G28560. [Q9SK02-2]
DR EnsemblPlants; AT2G28560.5; AT2G28560.5; AT2G28560. [Q9SK02-1]
DR EnsemblPlants; AT2G28560.6; AT2G28560.6; AT2G28560. [Q9SK02-1]
DR GeneID; 817404; -.
DR Gramene; AT2G28560.1; AT2G28560.1; AT2G28560. [Q9SK02-3]
DR Gramene; AT2G28560.2; AT2G28560.2; AT2G28560. [Q9SK02-1]
DR Gramene; AT2G28560.4; AT2G28560.4; AT2G28560. [Q9SK02-2]
DR Gramene; AT2G28560.5; AT2G28560.5; AT2G28560. [Q9SK02-1]
DR Gramene; AT2G28560.6; AT2G28560.6; AT2G28560. [Q9SK02-1]
DR KEGG; ath:AT2G28560; -.
DR Araport; AT2G28560; -.
DR TAIR; locus:2056583; AT2G28560.
DR eggNOG; KOG1433; Eukaryota.
DR InParanoid; Q9SK02; -.
DR OrthoDB; 1345899at2759; -.
DR PhylomeDB; Q9SK02; -.
DR PRO; PR:Q9SK02; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SK02; baseline and differential.
DR Genevisible; Q9SK02; AT.
DR GO; GO:0033063; C:Rad51B-Rad51C-Rad51D-XRCC2 complex; IBA:GO_Central.
DR GO; GO:0005657; C:replication fork; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008094; F:ATP-dependent activity, acting on DNA; IBA:GO_Central.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0000150; F:DNA strand exchange activity; ISS:TAIR.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IMP:TAIR.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR InterPro; IPR016467; DNA_recomb/repair_RecA-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR030548; RAD51B.
DR InterPro; IPR020588; RecA_ATP-bd.
DR PANTHER; PTHR46456; PTHR46456; 1.
DR Pfam; PF08423; Rad51; 1.
DR PIRSF; PIRSF005856; Rad51; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50162; RECA_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; DNA damage; DNA recombination;
KW DNA repair; DNA-binding; Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..370
FT /note="DNA repair protein RAD51 homolog 2"
FT /id="PRO_0000122944"
FT BINDING 109..116
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..32
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15952068"
FT /id="VSP_016462"
FT VAR_SEQ 270..275
FT /note="AKVKDE -> DFLCNS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15952068"
FT /id="VSP_016463"
FT VAR_SEQ 276..370
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15952068"
FT /id="VSP_016464"
FT VAR_SEQ 359..370
FT /note="HSDMINFHGDCS -> IVDPDYVALFRTF (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_016465"
FT CONFLICT 330
FT /note="H -> Q (in Ref. 1; CAD70704)"
FT /evidence="ECO:0000305"
FT CONFLICT 353
FT /note="T -> P (in Ref. 1; CAD70704)"
FT /evidence="ECO:0000305"
FT CONFLICT Q9SK02-3:362..364
FT /note="PDY -> FKD (in Ref. 1; CAD70704)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 370 AA; 40468 MW; EB7E5EAA5E35115A CRC64;
MANKLIGEMG LHTKISNIFA ARNIITAKDA LSMTEFELME LLDVGMKEIR SAISFISEAT
SPPCQSARSL LEKKVENEHL SGHLPTHLKG LDDTLCGGIP FGVLTELVGP PGIGKSQFCM
KLALSASFPV AYGGLDGRVI YIDVESKFSS RRVIEMGLES FPEVFHLKGM AQEMAGRILV
LRPTSLANFT ESIQELKNSI LQNQVKLLVI DSMTALLSGE NKPGAQRQPQ LGWHISFLKS
LAEFSRIPIV VTNQVRSQNR DETSQYSFQA KVKDEFKDNT KTYDSHLVAA LGINWAHAVT
IRLVLEAKSG QRIIKVAKSP MSPPLAFPFH ITSAGISLLS DNGTELKGPG INTIHARGHS
DMINFHGDCS
