RA51C_ARATH
ID RA51C_ARATH Reviewed; 363 AA.
AC Q8GXF0; O22144; Q8RY30; Q93X07; Q9FT75; Q9FT80;
DT 09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 09-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=DNA repair protein RAD51 homolog 3;
DE AltName: Full=DNA repair-recombination protein RAD51C;
DE Short=AtRAD51C;
GN Name=RAD51C; OrderedLocusNames=At2g45280; ORFNames=F4L23.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INDUCTION, SUBUNIT, TISSUE
RP SPECIFICITY, AND INTERACTION WITH XRCC3.
RC STRAIN=cv. Columbia; TISSUE=Flower bud, and Root;
RX PubMed=12139010; DOI=10.1023/a:1016047231597;
RA Osakabe K., Yoshioka T., Ichikawa H., Toki S.;
RT "Molecular cloning and characterization of RAD51-like genes from
RT Arabidopsis thaliana.";
RL Plant Mol. Biol. 50:71-81(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Siaud N.;
RT "A study of RAD51 homologues from Arabidopsis.";
RL Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-363 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
CC -!- FUNCTION: Involved in the homologous recombination repair (HRR) pathway
CC of double-stranded DNA breaks arising during DNA replication or induced
CC by DNA-damaging agents. {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Interacts with XRCC3.
CC {ECO:0000269|PubMed:12139010}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=Alpha;
CC IsoId=Q8GXF0-1; Sequence=Displayed;
CC Name=2; Synonyms=Beta;
CC IsoId=Q8GXF0-2; Sequence=VSP_011864, VSP_011865;
CC -!- TISSUE SPECIFICITY: Detected in various tissues. More expressed in
CC reproductive tissues than in vegetative tissues, with the highest level
CC in young flower buds. {ECO:0000269|PubMed:12139010}.
CC -!- INDUCTION: By genotoxic stress and by DNA damage.
CC {ECO:0000269|PubMed:12139010}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing donor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the RecA family. RAD51 subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC42893.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB062456; BAB64343.1; -; mRNA.
DR EMBL; AB073493; BAB70685.1; -; mRNA.
DR EMBL; AJ296174; CAC14294.1; -; Genomic_DNA.
DR EMBL; AJ299424; CAC14091.1; -; mRNA.
DR EMBL; AC002387; AAB82635.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC10533.1; -; Genomic_DNA.
DR EMBL; AY078935; AAL84941.1; -; mRNA.
DR EMBL; AK118275; BAC42893.1; ALT_INIT; mRNA.
DR PIR; F84888; F84888.
DR RefSeq; NP_566040.1; NM_130091.2. [Q8GXF0-1]
DR AlphaFoldDB; Q8GXF0; -.
DR SMR; Q8GXF0; -.
DR BioGRID; 4472; 1.
DR STRING; 3702.AT2G45280.2; -.
DR PRIDE; Q8GXF0; -.
DR EnsemblPlants; AT2G45280.1; AT2G45280.1; AT2G45280. [Q8GXF0-1]
DR GeneID; 819136; -.
DR Gramene; AT2G45280.1; AT2G45280.1; AT2G45280. [Q8GXF0-1]
DR KEGG; ath:AT2G45280; -.
DR Araport; AT2G45280; -.
DR eggNOG; KOG1434; Eukaryota.
DR HOGENOM; CLU_041732_1_1_1; -.
DR InParanoid; Q8GXF0; -.
DR OMA; RLILYWN; -.
DR PhylomeDB; Q8GXF0; -.
DR PRO; PR:Q8GXF0; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8GXF0; baseline and differential.
DR Genevisible; Q8GXF0; AT.
DR GO; GO:0033063; C:Rad51B-Rad51C-Rad51D-XRCC2 complex; IBA:GO_Central.
DR GO; GO:0033065; C:Rad51C-XRCC3 complex; IBA:GO_Central.
DR GO; GO:0005657; C:replication fork; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000707; P:meiotic DNA recombinase assembly; IBA:GO_Central.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IBA:GO_Central.
DR CDD; cd01123; Rad51_DMC1_radA; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR InterPro; IPR016467; DNA_recomb/repair_RecA-like.
DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR033925; Rad51_DMC1_RadA.
DR InterPro; IPR020588; RecA_ATP-bd.
DR Pfam; PF08423; Rad51; 1.
DR PIRSF; PIRSF005856; Rad51; 1.
DR SUPFAM; SSF47794; SSF47794; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50162; RECA_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; DNA damage; DNA recombination;
KW DNA repair; DNA-binding; Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..363
FT /note="DNA repair protein RAD51 homolog 3"
FT /id="PRO_0000122945"
FT BINDING 130..137
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 241
FT /note="K -> G (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12139010"
FT /id="VSP_011864"
FT VAR_SEQ 242..363
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12139010"
FT /id="VSP_011865"
FT CONFLICT 17
FT /note="L -> F (in Ref. 5; AAL84941)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="E -> K (in Ref. 2; CAC14091)"
FT /evidence="ECO:0000305"
FT CONFLICT 228
FT /note="N -> S (in Ref. 5; AAL84941)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 363 AA; 40215 MW; DC38925F9459F25C CRC64;
MISFGRRKSP AIEETSLATS VMEAWRLPLS PSIRGKLISA GYTCLSSIAS VSSSDLARDA
NITEEEAFEI LKLANQSCCN GSRSLINGAK NAWDMLHEEE SLPRITTSCS DLDNILGGGI
SCRDVTEIGG VPGIGKTQIG IQLSVNVQIP RECGGLGGKA IYIDTEGSFM VERALQIAEA
CVEDMEEYTG YMHKHFQANQ VQMKPEDILE NIFYFRVCSY TEQIALVNHL EKFISENKDV
KVVIVDSITF HFRQDYDDLA QRTRVLSEMA LKFMKLAKKF SLAVVLLNQV TTKFSEGSFQ
LALALGDSWS HSCTNRVILY WNGDERYAYI DKSPSLPSAS ASYTVTSRGL RNSSSSSKRV
KMM
