RA51D_BOVIN
ID RA51D_BOVIN Reviewed; 326 AA.
AC Q2HJ51;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=DNA repair protein RAD51 homolog 4;
DE AltName: Full=R51H3;
DE AltName: Full=RAD51 homolog D;
DE AltName: Full=RAD51-like protein 3;
GN Name=RAD51D; Synonyms=RAD51L3;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Uterus;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the homologous recombination repair (HRR) pathway
CC of double-stranded DNA breaks arising during DNA replication or induced
CC by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has
CC DNA-dependent ATPase activity. Part of the RAD51 paralog protein
CC complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon
CC DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of
CC RAD51 recruitment. BCDX2 binds predominantly to the intersection of the
CC four duplex arms of the Holliday junction and to junction of
CC replication forks. The BCDX2 complex was originally reported to bind
CC single-stranded DNA, single-stranded gaps in duplex DNA and
CC specifically to nicks in duplex DNA. Involved in telomere maintenance.
CC The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction
CC resolution by BLM (By similarity). {ECO:0000250|UniProtKB:O75771}.
CC -!- SUBUNIT: Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D
CC and XRCC2; the complex has a ring-like structure arranged into a flat
CC disc around a central channel. In the absence of DNA, the BCDX2
CC subcomplex XRCC2:RAD51D formed a multimeric ring structure; in the
CC presence of single-stranded DNA it formed a filamentous structure with
CC the ssDNA. Interacts with SWSAP1 and ZSWIM7; involved in homologous
CC recombination repair. Interacts with BLM; required for stimulation of
CC BLM activity by the BCDX2 subcomplex XRCC2:RAD51D (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RecA family. RAD51 subfamily. {ECO:0000305}.
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DR EMBL; BC113309; AAI13310.1; -; mRNA.
DR RefSeq; NP_001039769.1; NM_001046304.1.
DR AlphaFoldDB; Q2HJ51; -.
DR SMR; Q2HJ51; -.
DR STRING; 9913.ENSBTAP00000025404; -.
DR PaxDb; Q2HJ51; -.
DR Ensembl; ENSBTAT00000025404; ENSBTAP00000025404; ENSBTAG00000019082.
DR GeneID; 529507; -.
DR KEGG; bta:529507; -.
DR CTD; 5892; -.
DR VEuPathDB; HostDB:ENSBTAG00000019082; -.
DR VGNC; VGNC:33687; RAD51D.
DR eggNOG; KOG1433; Eukaryota.
DR GeneTree; ENSGT00940000159095; -.
DR HOGENOM; CLU_058452_0_0_1; -.
DR InParanoid; Q2HJ51; -.
DR OrthoDB; 1214879at2759; -.
DR TreeFam; TF101219; -.
DR Proteomes; UP000009136; Chromosome 19.
DR Bgee; ENSBTAG00000019082; Expressed in semen and 104 other tissues.
DR ExpressionAtlas; Q2HJ51; baseline.
DR GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR GO; GO:0000781; C:chromosome, telomeric region; ISS:UniProtKB.
DR GO; GO:0033063; C:Rad51B-Rad51C-Rad51D-XRCC2 complex; ISS:UniProtKB.
DR GO; GO:0005657; C:replication fork; ISS:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008094; F:ATP-dependent activity, acting on DNA; ISS:UniProtKB.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0043015; F:gamma-tubulin binding; ISS:UniProtKB.
DR GO; GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; ISS:UniProtKB.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IBA:GO_Central.
DR GO; GO:0042148; P:strand invasion; ISS:UniProtKB.
DR GO; GO:0000723; P:telomere maintenance; ISS:UniProtKB.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR InterPro; IPR016467; DNA_recomb/repair_RecA-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020588; RecA_ATP-bd.
DR Pfam; PF08423; Rad51; 1.
DR PIRSF; PIRSF005856; Rad51; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50162; RECA_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..326
FT /note="DNA repair protein RAD51 homolog 4"
FT /id="PRO_0000253607"
FT REGION 1..83
FT /note="preferentially binds ssDNA"
FT /evidence="ECO:0000250"
FT BINDING 107..114
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 326 AA; 34651 MW; BD0B5F327F6189F3 CRC64;
MGVLRAGLCP GLTQDMVQLL QSRGIKTVVD LVCADLEEVA QKCGLSYKAL VALRRVLLAQ
FSAFPFNGAD LYEELKTSTA ILSTGIGSLD KLLDAGLYTG EVTEIVGAPG SGKTQVCLCV
AAHVAHGLQQ NVLYIDSNGG LTASRILQLL QARTPDEEEQ AGALQRIQVV RAFDIFQMLD
VLQDLRGAVS QQVSSSSGTL KVVVVDSVAA VVAPLLGGQQ REGLALMMQL ARELKTLARD
LSVAVLVTNH MTRDRDSGQL KPALGRSWSF VPSTRLLLDS TQSSGSLGSW RVVCLTKSPR
LPTGCQETVD LGSLGTPAFQ GDHKGH
