ID   RAA2_CHLRE              Reviewed;         410 AA.
AC   Q9SMH4;
DT   10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Trans-splicing factor Raa2, chloroplastic;
DE   Flags: Precursor;
GN   Name=RAA2; Synonyms=MAA2;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND MUTAGENESIS.
RC   STRAIN=137c / CC-125;
RX   PubMed=10562560; DOI=10.1093/emboj/18.22.6481;
RA   Perron K., Goldschmidt-Clermont M., Rochaix J.-D.;
RT   "A factor related to pseudouridine synthases is required for chloroplast
RT   group II intron trans-splicing in Chlamydomonas reinhardtii.";
RL   EMBO J. 18:6481-6490(1999).
CC   -!- FUNCTION: Required for trans-splicing of exons 2 and 3 of the
CC       chloroplast encoded psaA mRNA (a group II intron). It is not known if
CC       this protein has pseudouridine activity; mutation of the potential
CC       active site residue does not cause loss of trans-splicing.
CC   -!- SUBUNIT: Possibly associated with other factors required for trans-
CC       splicing.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast. Note=Associated with low
CC       density membranes.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family.
CC       {ECO:0000305}.
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DR   EMBL; AJ243394; CAB62387.1; -; mRNA.
DR   RefSeq; XP_001701439.1; XM_001701387.1.
DR   AlphaFoldDB; Q9SMH4; -.
DR   SMR; Q9SMH4; -.
DR   STRING; 3055.EDO97436; -.
DR   PRIDE; Q9SMH4; -.
DR   EnsemblPlants; PNW71708; PNW71708; CHLRE_16g665750v5.
DR   GeneID; 5726983; -.
DR   Gramene; PNW71708; PNW71708; CHLRE_16g665750v5.
DR   eggNOG; KOG2529; Eukaryota.
DR   HOGENOM; CLU_671516_0_0_1; -.
DR   OrthoDB; 9734at3166; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR   PANTHER; PTHR23127; PTHR23127; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; mRNA processing; mRNA splicing; Plastid; Transit peptide.
FT   TRANSIT         1..46
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           47..410
FT                   /note="Trans-splicing factor Raa2, chloroplastic"
FT                   /id="PRO_0000029846"
FT   REGION          1..40
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          56..106
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        9..34
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        63..106
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         141
FT                   /note="D->E,S: No loss of function."
FT                   /evidence="ECO:0000269|PubMed:10562560"
FT   MUTAGEN         142
FT                   /note="K->I,R: No loss of function."
FT                   /evidence="ECO:0000269|PubMed:10562560"
FT   MUTAGEN         171
FT                   /note="D->G,S: No loss of function."
FT                   /evidence="ECO:0000269|PubMed:10562560"
FT   MUTAGEN         171
FT                   /note="Missing: No loss of function."
FT                   /evidence="ECO:0000269|PubMed:10562560"
SQ   SEQUENCE   410 AA;  44863 MW;  E8EF4FCD07BEC727 CRC64;
     MRTRAGAFFG KQRSTSPSGS STSASRQWLR SSPGRTQRPA AHRVLAVCVR NPARPAADHP
     LVSTTQAAAQ RQARQWDSRS KPPQLQPQLR HTHVSRQAQR RQQQQVQQQA EAGALVVPTV
     ITSFPEHLRP EVLANGVLLV DKPPHWEVPE VVAAVQRATG ADKVASVAPL DARASGLMLL
     CFGSATRLAP RVERAAKRYT GTLVLGGSSL SGDVRGGSFR AAQLPAEHLT DEDLREAAQG
     LVTAAAGAPV HGAVATGHQG GGGLALRVLP RTWRLRQLPS STEYYEERVE PSMRTLDMEL
     LDFRVWRESA LSHNDGAAGA EYDQHDQHKQ ELGRGLVWTR SPPHPRPVVL RFSALLVGRS
     HVRSLIAMYG RRLRTAACLD DLRRTEIGSF NVEEAWPLEA LVPVLQRHAH