RAA3_CHLRE
ID RAA3_CHLRE Reviewed; 1783 AA.
AC Q9FEC4;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=Trans-splicing factor Raa3, chloroplastic;
DE Flags: Precursor;
GN Name=RAA3;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND CHARACTERIZATION.
RC STRAIN=137c / CC-125;
RX PubMed=11285239; DOI=10.1093/emboj/20.7.1765;
RA Rivier C., Goldschmidt-Clermont M., Rochaix J.-D.;
RT "Identification of an RNA-protein complex involved in chloroplast group II
RT intron trans-splicing in Chlamydomonas reinhardtii.";
RL EMBO J. 20:1765-1773(2001).
CC -!- FUNCTION: Required for trans-splicing of exons 1 and 2 of the
CC chloroplast encoded psaA mRNA (a group II intron). May be required for
CC stability of the chloroplast RNA-protein complex in which it is found.
CC -!- SUBUNIT: Part of a 1700 kDa complex that includes the precursor RNA to
CC exon 1 and the tscA RNA.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC -!- DOMAIN: The N-terminal 453 amino acids are dispensable, while the C-
CC terminal 630 amino acids are required for function.
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DR EMBL; AF310675; AAG40000.1; -; Genomic_DNA.
DR EMBL; AF310674; AAG39999.1; -; mRNA.
DR AlphaFoldDB; Q9FEC4; -.
DR SMR; Q9FEC4; -.
DR STRING; 3055.EDP03491; -.
DR PRIDE; Q9FEC4; -.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR013584; RAP.
DR PROSITE; PS51286; RAP; 1.
PE 1: Evidence at protein level;
KW Chloroplast; mRNA processing; mRNA splicing; Plastid; Transit peptide.
FT TRANSIT 1..40
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 41..1783
FT /note="Trans-splicing factor Raa3, chloroplastic"
FT /id="PRO_0000022197"
FT DOMAIN 1713..1772
FT /note="RAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00619"
FT REGION 97..378
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 420..484
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 563..610
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 652..709
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 918..971
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1395..1427
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1476..1506
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1620..1639
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..156
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 259..276
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 336..378
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 420..453
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 469..484
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 576..603
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 652..686
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1783 AA; 180401 MW; 40F6206BA6EBDCDB CRC64;
MKADLATAKG SSPAFSAPRT YRARLLSRCL NKCFNTVLVS GGAAESRFRG GATVGKAGAH
DARGVGDLVD FLQQEATPYE VNVPPYLILP PLKSNEATTH RRAGDSGGQG PAAAGGRGQA
RHGRRQAAAS AATTVSAAPQ TGATKPAATA KTTPQRPRGS DADAGSRAQS QYGFGDPPGG
GALKGAVDAA SDAAPDVAAA SPAPAGISDQ LSTPACPPER EPQAGKPRAS GRAPAAPGVG
PQDVGGGSGA CAPAPDESHM GLTHRDQGHD ERISQTAGEA WKAGAVAAPP AAPTPSPPGL
AAAPTRLASS ALGTHSSDGD MRRAVPGRDT PSLSAVAGPV TLSGSSSSSS GRNSSSNSNT
STSSTSNGVT ITSNVGVNGA SPQERLMAAR RAVVTMQWNT HLGRRGRSFA PLPTGGMSIA
TSAASSSTSS ASSSSSMNDG SNAKKTSDAA VSLPVGQQPA AEQPHVPTAP GGPSQTGASA
VAAQAPSSAM PTAAMAATMG SATIGSAATL PTAAVVSSAA AEGTQPSGLL LAGGRPALLG
RTIQGRIARL QAAREALRAA RHARVGAAMQ PPPVQARPVQ GQSGQVPQVG QGPVQSQPGR
RQEPAASATK LHVADGLPAR PVQPAVSATD LQTDTATAAS APFPVSDASL GSTEPLAASA
PTTSLASASG PAIGTSSSNA HSSAAASEAA AGTVRAPTDT AAPAASPAAT APALASTPFA
TPAAAPLPEP PEVVAARDLL GRLSRYQPAG RDDGPAVLAP HARYSPAAYQ AAAAAAAAQP
QLLLAPLSLP QLAAVVAGYA AAGHRHEPLL EALAGVALAK AGGAGGIGGG AAGAVPRGQA
ASELKRSRLT FRAACVFLTA LARLGYRGGA VTRLAAALAV WLARQLNTGA VTPRAKWKGT
WLAAALWAYA TLEQLPAAAP TPPPPPASAA AASTAPRAAE SRPAAAPAPA EATATRTPLL
TAPQLAPQRA SASPDLELAR GGVLLFTEAA EAVRVAPGWL HLMDGREALW SLWAFRKAAA
AYGRGEGTAS GAVYVAAAGG YAMLQSGGAG GGGMGQAGGA GRGGAGAGAA ALPLVVAAPR
YEANPLVELK LADRATSLFP QLDPGQLAEA HVLLLECGLA EDELPQALTA LRRCVIARAD
SIRPQPLAVM VATLAVMQVR DVVWLSALAM ACRNKMINMS PDQIVAVLHA FGSVLRFHHL
LLFHAAAVVC SCPGMWRLGG MGAGEVLRLV GAFAATRHYE GRLLRAAAER MLQLGSTGST
AGQRAGLLQS LCSLHYRHNG LLRIVVADTF GLADLSPGSA GQPRNMGSSA KLAGAGTAAA
AGAAAPGLPP SLLVAVAEAC GQLQHRPPGL LQALHASRAA VWPRVGLAQR ATLCWALLVL
TGGLPAQYLP ADRPRDAKRH VRQSAAAAAA AAAAAQDQRQ EQHQQHQQEQ LLAKALVEYL
QALGAGVQRW PPPAPSSYHL QLLVACTVLA SCTPPPAPAH MQQQPQSSGT GCQKSRRRRM
RYRRSRGAVL QQQLKEELDK LPASAMQRAL EVQRRARSAA LGGWAHEVAS VVREVLQEAS
VDARADSEGR QPLPPHWLGQ PPATLLSAAV STGVEVCDGA MLVDVAVELE VEAGLPRPQV
KGRGRGRRTA ARATTDVQGA EQAVVTEEAA YEHSHPLRQR LQLALDLCPL PPPPPRTAAP
VLSAAYAPGA GGAGTSATAE VTANRTTGAP RSLAVGAAAG GAVIRNSRWL LSGAGALRRR
LLTHAGWLVV PVRERQWKDL RSAEQQRRVV REWLKAVLLH AQQ
