RAA4D_ARATH
ID RAA4D_ARATH Reviewed; 222 AA.
AC Q9LH50; Q9C7C6;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Ras-related protein RABA4d;
DE Short=AtRABA4d;
GN Name=RABA4D; OrderedLocusNames=At3g12160; ORFNames=F28J15.18, T21B14.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644670; DOI=10.1104/pp.013052;
RA Vernoud V., Horton A.C., Yang Z., Nielsen E.;
RT "Analysis of the small GTPase gene superfamily of Arabidopsis.";
RL Plant Physiol. 131:1191-1208(2003).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PI4KB1, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=19208902; DOI=10.1105/tpc.108.060277;
RA Szumlanski A.L., Nielsen E.;
RT "The Rab GTPase RabA4d regulates pollen tube tip growth in Arabidopsis
RT thaliana.";
RL Plant Cell 21:526-544(2009).
CC -!- FUNCTION: Intracellular vesicle trafficking and protein transport.
CC Plays an important role in the regulation of pollen tube tip growth.
CC {ECO:0000269|PubMed:19208902}.
CC -!- SUBUNIT: Interacts with PI4KB1. {ECO:0000269|PubMed:19208902}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC {ECO:0000305|PubMed:19208902}; Lipid-anchor
CC {ECO:0000305|PubMed:19208902}. Note=Associated with membrane
CC compartments that localize to the tips of growing pollen tubes.
CC -!- TISSUE SPECIFICITY: Specifically expressed in pollen and localized to
CC the tips of growing pollen tubes. {ECO:0000269|PubMed:19208902}.
CC -!- DISRUPTION PHENOTYPE: Defects in pollen tube growth and altered
CC deposition of cell wall components. {ECO:0000269|PubMed:19208902}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51053.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC069472; AAG51065.1; -; Genomic_DNA.
DR EMBL; AC069473; AAG51053.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP002063; BAB01966.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75160.1; -; Genomic_DNA.
DR EMBL; DQ056590; AAY78738.1; -; mRNA.
DR RefSeq; NP_187823.1; NM_112051.2.
DR AlphaFoldDB; Q9LH50; -.
DR SMR; Q9LH50; -.
DR STRING; 3702.AT3G12160.1; -.
DR PaxDb; Q9LH50; -.
DR PRIDE; Q9LH50; -.
DR ProteomicsDB; 226098; -.
DR EnsemblPlants; AT3G12160.1; AT3G12160.1; AT3G12160.
DR GeneID; 820393; -.
DR Gramene; AT3G12160.1; AT3G12160.1; AT3G12160.
DR KEGG; ath:AT3G12160; -.
DR Araport; AT3G12160; -.
DR TAIR; locus:2082269; AT3G12160.
DR eggNOG; KOG0087; Eukaryota.
DR HOGENOM; CLU_041217_23_0_1; -.
DR InParanoid; Q9LH50; -.
DR OMA; PNQDMNA; -.
DR OrthoDB; 1133775at2759; -.
DR PhylomeDB; Q9LH50; -.
DR PRO; PR:Q9LH50; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LH50; baseline and differential.
DR Genevisible; Q9LH50; AT.
DR GO; GO:0045177; C:apical part of cell; IDA:TAIR.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0070382; C:exocytic vesicle; IDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0090404; C:pollen tube tip; IDA:TAIR.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0019900; F:kinase binding; IPI:UniProtKB.
DR GO; GO:0048868; P:pollen tube development; IGI:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IDA:TAIR.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0080092; P:regulation of pollen tube growth; IMP:TAIR.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; GTP-binding; Lipoprotein; Membrane;
KW Nucleotide-binding; Prenylation; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..222
FT /note="Ras-related protein RABA4d"
FT /id="PRO_0000407347"
FT MOTIF 44..52
FT /note="Effector region"
FT /evidence="ECO:0000250"
FT BINDING 22..29
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 70..74
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 128..131
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 158..159
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT LIPID 218
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 219
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 222 AA; 24720 MW; 5BED5C804F6B1F09 CRC64;
MSNLYGDYNQ KIDYVFKVVL IGDSAVGKTQ LLARFARNEF SVDSKATIGV EFQTKTLVID
NKTVKAQIWD TAGQERYRAV TSAYYRGAVG AMLVYDMTKR QSFDHMAKWL EELRGHADKN
IVIMLIGNKC DLGSLRAVPT EDAQEFAQRE NLFFMETSAL EATNVETAFL TILTEIYRII
SKKSLTADDD DADGNSSLLK GTRIIIPSEQ ESGKRGGCCG KS
