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RAAS_MYCTU
ID   RAAS_MYCTU              Reviewed;         212 AA.
AC   O86312; F2GFW9; I6XXG2; Q7D8L0;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 154.
DE   RecName: Full=HTH-type transcriptional regulatory protein RaaS {ECO:0000305};
DE   AltName: Full=Regulator of antimicrobial-assisted survival {ECO:0000303|PubMed:24590482};
GN   Name=raaS {ECO:0000303|PubMed:24590482};
GN   OrderedLocusNames=Rv1219c {ECO:0000312|EMBL:CCP43975.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [3]
RP   FUNCTION IN VIRULENCE, AND DISRUPTION PHENOTYPE.
RX   PubMed=24590482; DOI=10.1128/aac.02774-13;
RA   Turapov O., Waddell S.J., Burke B., Glenn S., Sarybaeva A.A., Tudo G.,
RA   Labesse G., Young D.I., Young M., Andrew P.W., Butcher P.D.,
RA   Cohen-Gonsaud M., Mukamolova G.V.;
RT   "Antimicrobial treatment improves mycobacterial survival in nonpermissive
RT   growth conditions.";
RL   Antimicrob. Agents Chemother. 58:2798-2806(2014).
RN   [4]
RP   FUNCTION, DNA-BINDING, ACTIVITY REGULATION, SUBUNIT, INTERACTION WITH LONG
RP   CHAIN ACYL-COA, AND MUTAGENESIS OF ARG-144 AND TYR-174.
RX   PubMed=25012658; DOI=10.1074/jbc.m114.577338;
RA   Turapov O., Waddell S.J., Burke B., Glenn S., Sarybaeva A.A., Tudo G.,
RA   Labesse G., Young D.I., Young M., Andrew P.W., Butcher P.D.,
RA   Cohen-Gonsaud M., Mukamolova G.V.;
RT   "Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS
RT   (Rv1219c) with DNA in mycobacteria.";
RL   J. Biol. Chem. 289:25241-25249(2014).
RN   [5] {ECO:0007744|PDB:4NN1}
RP   X-RAY CRYSTALLOGRAPHY (2.99 ANGSTROMS), FUNCTION, DNA-BINDING, SUBUNIT,
RP   INTERACTION WITH DRUGS, INDUCTION, AND DOMAIN.
RC   STRAIN=H37Rv;
RX   PubMed=24424575; DOI=10.1002/pro.2424;
RA   Kumar N., Radhakrishnan A., Wright C.C., Chou T.H., Lei H.T., Bolla J.R.,
RA   Tringides M.L., Rajashankar K.R., Su C.C., Purdy G.E., Yu E.W.;
RT   "Crystal structure of the transcriptional regulator Rv1219c of
RT   Mycobacterium tuberculosis.";
RL   Protein Sci. 23:423-432(2014).
CC   -!- FUNCTION: Regulates the expression of the Rv1217c-Rv1218c multidrug
CC       efflux system and its own expression. Acts by binding to promoter
CC       regions of Rv1219c and upstream of the Rv1218c gene (PubMed:24424575).
CC       Important for survival in prolonged stationary phase and during
CC       macrophage infection (PubMed:24590482). May be used to eliminate non-
CC       growing mycobacteria (PubMed:25012658). {ECO:0000269|PubMed:24424575,
CC       ECO:0000269|PubMed:24590482, ECO:0000269|PubMed:25012658}.
CC   -!- ACTIVITY REGULATION: Interaction with long chain acyl-CoA derivatives
CC       (oleoyl-CoA and, to lesser extent, stearoyl-CoA) prevents binding to
CC       DNA, leading to the expression of the target genes. Long chain acyl-CoA
CC       derivatives may serve as biological indicators of the bacterial
CC       metabolic state. {ECO:0000269|PubMed:25012658}.
CC   -!- SUBUNIT: Homodimer (PubMed:24424575, PubMed:25012658). Interacts with
CC       long chain acyl-CoA derivatives (PubMed:25012658). Interacts with
CC       several drugs such rhodamine 6G, ethidium and safranin O
CC       (PubMed:24424575). {ECO:0000269|PubMed:24424575,
CC       ECO:0000269|PubMed:25012658}.
CC   -!- INDUCTION: Expression is autoregulated. {ECO:0000269|PubMed:24424575}.
CC   -!- DOMAIN: Contains an N-terminal DNA-binding domain and a C-terminal
CC       ligand-binding domain, which can accommodate a variety of structurally
CC       unrelated antimicrobial agents. The C-terminal domain is also involved
CC       in dimerization. {ECO:0000269|PubMed:24424575}.
CC   -!- DISRUPTION PHENOTYPE: Knockout mutant is impaired in long-term survival
CC       at stationary phase and does not persist during macrophage infection.
CC       {ECO:0000269|PubMed:24590482}.
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DR   EMBL; AL123456; CCP43975.1; -; Genomic_DNA.
DR   RefSeq; NP_215735.1; NC_000962.3.
DR   RefSeq; WP_003406254.1; NZ_NVQJ01000039.1.
DR   PDB; 4NN1; X-ray; 2.99 A; A=1-212.
DR   PDBsum; 4NN1; -.
DR   AlphaFoldDB; O86312; -.
DR   SMR; O86312; -.
DR   STRING; 83332.Rv1219c; -.
DR   PaxDb; O86312; -.
DR   PRIDE; O86312; -.
DR   DNASU; 888582; -.
DR   GeneID; 45425189; -.
DR   GeneID; 888582; -.
DR   KEGG; mtu:Rv1219c; -.
DR   PATRIC; fig|83332.111.peg.1362; -.
DR   TubercuList; Rv1219c; -.
DR   eggNOG; COG1309; Bacteria.
DR   OMA; IEQFGEH; -.
DR   PhylomeDB; O86312; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   InterPro; IPR041484; TetR_C_25.
DR   Pfam; PF17933; TetR_C_25; 1.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..212
FT                   /note="HTH-type transcriptional regulatory protein RaaS"
FT                   /id="PRO_0000447332"
FT   DOMAIN          6..65
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        28..47
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   MUTAGEN         144
FT                   /note="R->A: Reduces DNA binding affinity and ligand
FT                   binding."
FT                   /evidence="ECO:0000269|PubMed:25012658"
FT   MUTAGEN         174
FT                   /note="Y->A: Reduces DNA binding affinity and ligand
FT                   binding."
FT                   /evidence="ECO:0000269|PubMed:25012658"
FT   HELIX           7..23
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   HELIX           29..36
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   HELIX           40..47
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   HELIX           50..74
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   HELIX           79..86
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   HELIX           87..91
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   HELIX           92..103
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   HELIX           107..129
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   HELIX           139..159
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   STRAND          161..163
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   HELIX           167..177
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   HELIX           179..188
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   STRAND          190..193
FT                   /evidence="ECO:0007829|PDB:4NN1"
FT   HELIX           195..209
FT                   /evidence="ECO:0007829|PDB:4NN1"
SQ   SEQUENCE   212 AA;  23181 MW;  8FBC03B909EA88C0 CRC64;
     MRSADLTAHA RIREAAIEQF GRHGFGVGLR AIAEAAGVSA ALVIHHFGSK EGLRKACDDF
     VAEEIRSSKA AALKSNDPTT WLAQMAEIES YAPLMAYLVR SMQSGGELAK MLWQKMIDNA
     EEYLDEGVRA GTVKPSRDPR ARARFLAITG GGGFLLYLQM HENPTDLRAA LRDYAHDMVL
     PSLEVYTEGL LADRAMYEAF LAEAQQGEAH VG
 
 
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