RAB18_RAT
ID RAB18_RAT Reviewed; 206 AA.
AC Q5EB77;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Ras-related protein Rab-18;
DE Flags: Precursor;
GN Name=Rab18;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Required for the localization of ZFYVE1 to lipid droplets and
CC for its function in mediating the formation of endoplasmic reticulum-
CC lipid droplets (ER-LD) contacts (By similarity). Plays a role in apical
CC endocytosis/recycling (By similarity). Plays a key role in eye and
CC brain development and neurodegeneration (By similarity).
CC {ECO:0000250|UniProtKB:Q8MXS1, ECO:0000250|UniProtKB:Q9NP72}.
CC -!- SUBUNIT: Interacts (in GTP-bound form) with ZFYVE1 (By similarity).
CC Interacts with ZW10 and this interaction is enhanced in the presence of
CC ZFYVE1 (By similarity). Interacts with BSCL2 (By similarity).
CC {ECO:0000250|UniProtKB:Q9NP72}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000250|UniProtKB:P35293}. Lipid droplet
CC {ECO:0000250|UniProtKB:Q9NP72}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; BC089957; AAH89957.1; -; mRNA.
DR RefSeq; NP_001012486.1; NM_001012468.1.
DR AlphaFoldDB; Q5EB77; -.
DR SMR; Q5EB77; -.
DR BioGRID; 258615; 2.
DR IntAct; Q5EB77; 4.
DR MINT; Q5EB77; -.
DR STRING; 10116.ENSRNOP00000025828; -.
DR iPTMnet; Q5EB77; -.
DR PhosphoSitePlus; Q5EB77; -.
DR jPOST; Q5EB77; -.
DR PaxDb; Q5EB77; -.
DR PRIDE; Q5EB77; -.
DR GeneID; 307039; -.
DR KEGG; rno:307039; -.
DR UCSC; RGD:1308905; rat.
DR CTD; 22931; -.
DR RGD; 1308905; Rab18.
DR VEuPathDB; HostDB:ENSRNOG00000018972; -.
DR eggNOG; KOG0080; Eukaryota.
DR HOGENOM; CLU_041217_10_7_1; -.
DR InParanoid; Q5EB77; -.
DR OMA; HRTLFIE; -.
DR OrthoDB; 1247169at2759; -.
DR PhylomeDB; Q5EB77; -.
DR TreeFam; TF313448; -.
DR Reactome; R-RNO-6798695; Neutrophil degranulation.
DR Reactome; R-RNO-6811436; COPI-independent Golgi-to-ER retrograde traffic.
DR Reactome; R-RNO-8873719; RAB geranylgeranylation.
DR Reactome; R-RNO-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR PRO; PR:Q5EB77; -.
DR Proteomes; UP000002494; Chromosome 17.
DR Bgee; ENSRNOG00000018972; Expressed in stomach and 19 other tissues.
DR Genevisible; Q5EB77; RN.
DR GO; GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0071782; C:endoplasmic reticulum tubular network; ISO:RGD.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0005811; C:lipid droplet; ISS:UniProtKB.
DR GO; GO:0045202; C:synapse; IDA:SynGO.
DR GO; GO:0019003; F:GDP binding; ISS:UniProtKB.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; ISS:UniProtKB.
DR GO; GO:0071786; P:endoplasmic reticulum tubular network organization; ISO:RGD.
DR GO; GO:0001654; P:eye development; ISS:UniProtKB.
DR GO; GO:0051170; P:import into nucleus; ISO:RGD.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0034389; P:lipid droplet organization; ISO:RGD.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cell membrane; Developmental protein; GTP-binding;
KW Lipid droplet; Lipoprotein; Membrane; Methylation; Nucleotide-binding;
KW Palmitate; Phosphoprotein; Prenylation; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..203
FT /note="Ras-related protein Rab-18"
FT /id="PRO_0000121196"
FT PROPEP 204..206
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000370764"
FT MOTIF 37..45
FT /note="Effector region"
FT /evidence="ECO:0000250"
FT BINDING 15..23
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 63..67
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 122..125
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 151..153
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9NP72"
FT MOD_RES 144
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P35293"
FT MOD_RES 203
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000255"
FT LIPID 199
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 203
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 206 AA; 22976 MW; D1A398067A6B9B77 CRC64;
MDEDVLTTLK ILIIGESGVG KSSLLLRFTD DTFDPELAAT IGVDFKVKTI SVDGNKAKLA
IWDTAGQERF RTLTPSYYRG AQGVILVYDV TRRDTFVKLD NWLNELETYC TRNDIVNMLV
GNKIDKENRE VDRNEGLKFA RKHSMLFIEA SAKTCDGVQC AFEELVEKII QTPGLWESEN
QNKGVKLSPR EESHGGGACG GYCSVL
