RAB20_MOUSE
ID RAB20_MOUSE Reviewed; 233 AA.
AC P35295;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Ras-related protein Rab-20;
GN Name=Rab20;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RC TISSUE=Kidney;
RX PubMed=7706395; DOI=10.1242/jcs.107.12.3437;
RA Luetcke A., Parton R.G., Murphy C., Olkkonen V.M., Dupree P., Valencia A.,
RA Simons K., Zerial M.;
RT "Cloning and subcellular localization of novel rab proteins reveals
RT polarized and cell type-specific expression.";
RL J. Cell Sci. 107:3437-3448(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-60.
RC TISSUE=Kidney;
RX PubMed=1555775; DOI=10.1016/0378-1119(92)90387-5;
RA Chavrier P., Simons K., Zerial M.;
RT "The complexity of the Rab and Rho GTP-binding protein subfamilies revealed
RT by a PCR cloning approach.";
RL Gene 112:261-264(1992).
CC -!- FUNCTION: Plays a role in apical endocytosis/recycling. Plays a role in
CC the maturation and acidification of phagosomes that engulf pathogens,
CC such as S.aureus and Mycobacterium. Plays a role in the fusion of
CC phagosomes with lysosomes (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, phagosome
CC {ECO:0000250|UniProtKB:Q9NX57}. Cytoplasmic vesicle, phagosome membrane
CC {ECO:0000305}; Lipid-anchor {ECO:0000305}; Cytoplasmic side
CC {ECO:0000305}. Golgi apparatus {ECO:0000250|UniProtKB:Q9NX57}.
CC Note=Recruited to phagosomes containing S.aureus or Mycobacterium (By
CC similarity). Highly enriched on apical endocytic structures in
CC polarized epithelial cells of kidney proximal tubules (PubMed:7706395).
CC {ECO:0000250|UniProtKB:Q9NX57, ECO:0000269|PubMed:7706395}.
CC -!- TISSUE SPECIFICITY: Present in a variety of tissues, but not in brain.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; X80332; CAA56582.1; -; mRNA.
DR EMBL; M79310; AAK14834.1; -; mRNA.
DR CCDS; CCDS22095.1; -.
DR PIR; I48728; S46950.
DR RefSeq; NP_035357.1; NM_011227.1.
DR AlphaFoldDB; P35295; -.
DR SMR; P35295; -.
DR STRING; 10090.ENSMUSP00000033900; -.
DR iPTMnet; P35295; -.
DR PhosphoSitePlus; P35295; -.
DR PaxDb; P35295; -.
DR PRIDE; P35295; -.
DR ProteomicsDB; 255064; -.
DR Antibodypedia; 25565; 293 antibodies from 28 providers.
DR DNASU; 19332; -.
DR Ensembl; ENSMUST00000033900; ENSMUSP00000033900; ENSMUSG00000031504.
DR GeneID; 19332; -.
DR KEGG; mmu:19332; -.
DR UCSC; uc009kvd.1; mouse.
DR CTD; 55647; -.
DR MGI; MGI:102789; Rab20.
DR VEuPathDB; HostDB:ENSMUSG00000031504; -.
DR eggNOG; KOG0092; Eukaryota.
DR GeneTree; ENSGT00940000161024; -.
DR HOGENOM; CLU_041217_12_0_1; -.
DR InParanoid; P35295; -.
DR OMA; HDCIYAL; -.
DR OrthoDB; 1340129at2759; -.
DR PhylomeDB; P35295; -.
DR TreeFam; TF331574; -.
DR Reactome; R-MMU-8873719; RAB geranylgeranylation.
DR BioGRID-ORCS; 19332; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Rab20; mouse.
DR PRO; PR:P35295; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; P35295; protein.
DR Bgee; ENSMUSG00000031504; Expressed in cleaving embryo and 137 other tissues.
DR ExpressionAtlas; P35295; baseline and differential.
DR Genevisible; P35295; MM.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0045335; C:phagocytic vesicle; IDA:MGI.
DR GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0071346; P:cellular response to interferon-gamma; IDA:MGI.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0090383; P:phagosome acidification; ISS:UniProtKB.
DR GO; GO:0090385; P:phagosome-lysosome fusion; ISS:UniProtKB.
DR CDD; cd04126; Rab20; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR041836; Rab20.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 2: Evidence at transcript level;
KW Cytoplasmic vesicle; Golgi apparatus; GTP-binding; Lipoprotein; Membrane;
KW Nucleotide-binding; Prenylation; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..233
FT /note="Ras-related protein Rab-20"
FT /id="PRO_0000121203"
FT REGION 119..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 33..41
FT /note="Effector region"
FT /evidence="ECO:0000250"
FT BINDING 12..19
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 55..59
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 113..116
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT LIPID 231
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 232
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 233 AA; 25989 MW; 3A2E70A18D5280A9 CRC64;
MRKPDGKIVL LGDMNVGKTS LLQRYMERRF PDTVSTVGGA FYLKQWRSFN ISIWDTAGRE
QFHGLGSLYC RGAAAIILTY DVNHPQSLFE LEDRFLGLTE TANNDCLFAI VGNKVDLTSE
RDTEGGEKEG PASGKVGSCV STKVPKQVQP EDAVALYKKI LKYKMLDERE MPAAEQMCFE
TSAKTGYNVD LLFETLFDLV VPMIMRQRAE ESDQTVDIAS CKTPKQTRSG CCA
