RAB21_DICDI
ID RAB21_DICDI Reviewed; 212 AA.
AC P34142; Q54LF6;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Ras-related protein Rab-21;
DE AltName: Full=Ras-related protein RabB;
GN Name=rab21; Synonyms=rabB; ORFNames=DDB_G0286553;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=AX3;
RX PubMed=8294041; DOI=10.1016/0378-1119(93)90447-b;
RA Bush J.M. IV, Franek K., Daniel J.M., Spiegelman G.B., Weeks G.,
RA Cardelli J.A.;
RT "Cloning and characterization of five novel Dictyostelium discoideum rab-
RT related genes.";
RL Gene 136:55-60(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP INDUCTION, INTERACTION WITH LIMF AND CHLIM, AND MUTAGENESIS OF THR-21 AND
RP GLN-66.
RX PubMed=15962002; DOI=10.1038/sj.emboj.7600716;
RA Khurana T., Brzostowski J.A., Kimmel A.R.;
RT "A Rab21/LIM-only/CH-LIM complex regulates phagocytosis via both activating
RT and inhibitory mechanisms.";
RL EMBO J. 24:2254-2264(2005).
CC -!- FUNCTION: Involved in the regulation of phagocytosis.
CC -!- SUBUNIT: Interacts with LIM domain proteins limF and ChLim.
CC {ECO:0000269|PubMed:15962002}.
CC -!- INTERACTION:
CC P34142; Q86I44: limF; NbExp=4; IntAct=EBI-1808935, EBI-1808928;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC -!- INDUCTION: Activated by limf and repressed by ChLim.
CC {ECO:0000269|PubMed:15962002}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L21012; AAC37383.1; -; mRNA.
DR EMBL; AAFI02000089; EAL64032.1; -; Genomic_DNA.
DR RefSeq; XP_637605.1; XM_632513.1.
DR AlphaFoldDB; P34142; -.
DR SMR; P34142; -.
DR IntAct; P34142; 2.
DR STRING; 44689.DDB0191253; -.
DR PaxDb; P34142; -.
DR EnsemblProtists; EAL64032; EAL64032; DDB_G0286553.
DR GeneID; 8625745; -.
DR KEGG; ddi:DDB_G0286553; -.
DR dictyBase; DDB_G0286553; rab21.
DR eggNOG; KOG0088; Eukaryota.
DR HOGENOM; CLU_041217_10_2_1; -.
DR InParanoid; P34142; -.
DR OMA; DICLCIA; -.
DR PhylomeDB; P34142; -.
DR Reactome; R-DDI-8873719; RAB geranylgeranylation.
DR Reactome; R-DDI-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR PRO; PR:P34142; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005769; C:early endosome; IBA:GO_Central.
DR GO; GO:0005811; C:lipid droplet; HDA:dictyBase.
DR GO; GO:0140220; C:pathogen-containing vacuole; HDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0030866; P:cortical actin cytoskeleton organization; IMP:dictyBase.
DR GO; GO:0046847; P:filopodium assembly; IMP:dictyBase.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0006909; P:phagocytosis; IEA:UniProtKB-KW.
DR GO; GO:0050766; P:positive regulation of phagocytosis; IMP:dictyBase.
DR GO; GO:0032482; P:Rab protein signal transduction; IEA:InterPro.
DR CDD; cd04123; Rab21; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR041833; Rab21.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW Cell membrane; GTP-binding; Lipoprotein; Membrane; Nucleotide-binding;
KW Phagocytosis; Prenylation; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..212
FT /note="Ras-related protein Rab-21"
FT /id="PRO_0000121269"
FT REGION 181..212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..195
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 14..21
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 62..66
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 120..123
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT LIPID 209
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 210
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT MUTAGEN 21
FT /note="T->N: Dominant negative; alters cortical F-actin
FT organization and decreases the rate of phagocytosis by 2-
FT fold."
FT /evidence="ECO:0000269|PubMed:15962002"
FT MUTAGEN 66
FT /note="Q->L: Alters cortical F-actin organization and
FT increases the rate of phagocytosis by 2-fold."
FT /evidence="ECO:0000269|PubMed:15962002"
FT CONFLICT 204
FT /note="N -> NN (in Ref. 1; AAC37383)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 212 AA; 23490 MW; 77148343ECA2E14D CRC64;
MTDTEKSFKV VLLGEGCVGK TSIVFRYIDN IFNDKHLMTQ HAGFFQKHIN IGGKRICLTI
WDTAGQERFH ALGPIYYRGS QGALVVYDIT DNDSFIKAKN WIKELKTMLG NDISLCIIGN
KCDLEKTRVI PLADAEAYAK SVGAIHYSTS AKLNKGIEEL FLDLTRRMIL NSSGVVIHSN
TNTTGQTTNR SERIPIVPDS DSGNKQPGCC SN
