RAB3I_MOUSE
ID RAB3I_MOUSE Reviewed; 428 AA.
AC Q68EF0;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Rab-3A-interacting protein;
DE Short=Rab3A-interacting protein;
DE AltName: Full=Rabin-3;
DE AltName: Full=SSX2-interacting protein;
GN Name=Rab3ip {ECO:0000312|EMBL:AAH80289.1}; Synonyms=Rabin8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000312|EMBL:AAH80289.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAH80289.1};
RC TISSUE=Brain {ECO:0000312|EMBL:AAH80289.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215 AND SER-248, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate
CC RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting
CC inactive GDP-bound Rab proteins into their active GTP-bound form.
CC Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or
CC RAB5. Modulates actin organization and promotes polarized transport of
CC RAB8A-specific vesicles to the cell surface. Together with RAB11A,
CC RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP
CC promotes transcytosis of PODXL to the apical membrane initiation sites
CC (AMIS), apical surface formation and lumenogenesis.
CC {ECO:0000250|UniProtKB:Q96QF0}.
CC -!- SUBUNIT: Interacts with the N-terminal region of SSX2 (By similarity).
CC Interacts with the GDP-bound forms of RAB8A and RAB8B (By similarity).
CC The interaction with RAB8A is prevented by phosphorylation of RAB8A at
CC 'Thr-72' (By similarity). Interacts with the GDP-bound forms of RAB3A
CC and RAB3D (By similarity). Interacts with DCDC1 (By similarity).
CC Interacts (via the N-terminal region) with TRAPPC14; this interaction
CC mediates RAB3IP association with the TRAPP II complex (By similarity).
CC {ECO:0000250|UniProtKB:Q62739, ECO:0000250|UniProtKB:Q96QF0}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q96QF0}. Nucleus
CC {ECO:0000250|UniProtKB:Q96QF0}. Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q96QF0}. Cell projection, lamellipodium
CC {ECO:0000250|UniProtKB:Q96QF0}. Vesicle {ECO:0000250|UniProtKB:Q96QF0}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC {ECO:0000250|UniProtKB:Q96QF0}. Note=Predominantly cytoplasmic but a
CC small proportion colocalizes with SSX2 in the nucleus. Activation of
CC protein kinase C results in redistribution to the periphery of
CC lamellipodia. {ECO:0000250|UniProtKB:Q96QF0}.
CC -!- SIMILARITY: Belongs to the SEC2 family. {ECO:0000255}.
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DR EMBL; BC080289; AAH80289.1; -; mRNA.
DR CCDS; CCDS24186.1; -.
DR RefSeq; NP_001003950.1; NM_001003950.2.
DR AlphaFoldDB; Q68EF0; -.
DR SMR; Q68EF0; -.
DR BioGRID; 229736; 3.
DR IntAct; Q68EF0; 3.
DR MINT; Q68EF0; -.
DR STRING; 10090.ENSMUSP00000020375; -.
DR iPTMnet; Q68EF0; -.
DR PhosphoSitePlus; Q68EF0; -.
DR EPD; Q68EF0; -.
DR jPOST; Q68EF0; -.
DR MaxQB; Q68EF0; -.
DR PaxDb; Q68EF0; -.
DR PeptideAtlas; Q68EF0; -.
DR PRIDE; Q68EF0; -.
DR ProteomicsDB; 300226; -.
DR Antibodypedia; 29437; 235 antibodies from 24 providers.
DR DNASU; 216363; -.
DR Ensembl; ENSMUST00000020375; ENSMUSP00000020375; ENSMUSG00000064181.
DR GeneID; 216363; -.
DR KEGG; mmu:216363; -.
DR UCSC; uc007hcj.1; mouse.
DR CTD; 117177; -.
DR MGI; MGI:105933; Rab3ip.
DR VEuPathDB; HostDB:ENSMUSG00000064181; -.
DR eggNOG; KOG4324; Eukaryota.
DR GeneTree; ENSGT00940000157998; -.
DR HOGENOM; CLU_038204_2_0_1; -.
DR InParanoid; Q68EF0; -.
DR PhylomeDB; Q68EF0; -.
DR TreeFam; TF313748; -.
DR Reactome; R-MMU-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-MMU-5620916; VxPx cargo-targeting to cilium.
DR Reactome; R-MMU-5620922; BBSome-mediated cargo-targeting to cilium.
DR Reactome; R-MMU-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR BioGRID-ORCS; 216363; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Rab3ip; mouse.
DR PRO; PR:Q68EF0; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q68EF0; protein.
DR Bgee; ENSMUSG00000064181; Expressed in right kidney and 260 other tissues.
DR ExpressionAtlas; Q68EF0; baseline and differential.
DR Genevisible; Q68EF0; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0070319; C:Golgi to plasma membrane transport vesicle; IBA:GO_Central.
DR GO; GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:1990635; C:proximal dendrite; ISO:MGI.
DR GO; GO:0051020; F:GTPase binding; ISO:MGI.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0060271; P:cilium assembly; ISO:MGI.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0051490; P:negative regulation of filopodium assembly; ISO:MGI.
DR GO; GO:0033365; P:protein localization to organelle; ISO:MGI.
DR GO; GO:0006612; P:protein targeting to membrane; ISO:MGI.
DR InterPro; IPR040351; RAB3IL/RAB3IP/Sec2.
DR PANTHER; PTHR14430; PTHR14430; 2.
PE 1: Evidence at protein level;
KW Cell projection; Coiled coil; Cytoplasm; Cytoskeleton;
KW Guanine-nucleotide releasing factor; Nucleus; Phosphoprotein;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..428
FT /note="Rab-3A-interacting protein"
FT /id="PRO_0000097145"
FT COILED 149..212
FT /evidence="ECO:0000255"
FT MOD_RES 147
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96QF0"
FT MOD_RES 215
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 218
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96QF0"
FT MOD_RES 240
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96QF0"
FT MOD_RES 248
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 428 AA; 47133 MW; 443F79CDDACD9D3F CRC64;
MANDPLEGFH EVNLASPTSP DLLGVCDPGT QEQTTSPSVI YRPHPSTLCA APLQANALDL
SDLPTQPVYS SPRHFNCAEV SNISAHAPDP ASSVPSAVAS GLTKLTSRKD SCNAEREFLQ
GATITEASAG NDDIFGLSTD SLSRLRSPSV LEVREKGYER LKEELAKAQR EAHKMVREAN
VKQATAEKQL KEAQGKIDVL QAEVAALKTL VLSSSPTSPT QEPLAAAKTP FKRGHTRNKS
TSSAMGGSHQ DLSVIQPIVK DCKEADLSLY NEFRSWKDEP TMDRTCPFLD KIYQEDIFPC
LTFAKSELAS AVLEAVENNT LSIEPVGLQP IRFVKASAVE CGGPKKCALT GQSKPCRHRI
RLGDSSCCYY ISPFCRYRIT SVCNFFTYIR YIQQGLVKQQ DVDQMFWEVM QLRKEMSLAK
LGYFKEEL
