RAB43_RAT
ID RAB43_RAT Reviewed; 210 AA.
AC Q53B90;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Ras-related protein Rab-43;
GN Name=Rab43;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley;
RA Zhong C., Zhou G., Ji G., Yu L.;
RT "Cloning, expression pattern and homology modeling of novel rat RAB
RT genes.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The small GTPases Rab are key regulators of intracellular
CC membrane trafficking, from the formation of transport vesicles to their
CC fusion with membranes. Rabs cycle between an inactive GDP-bound form
CC and an active GTP-bound form that is able to recruit to membranes
CC different set of downstream effectors directly responsible for vesicle
CC formation, movement, tethering and fusion. The low intrinsic GTPase
CC activity of RAB43 is activated by USP6NL. Involved in retrograde
CC transport from the endocytic pathway to the Golgi apparatus. Involved
CC in the transport of Shiga toxin from early and recycling endosomes to
CC the trans-Golgi network. Required for the structural integrity of the
CC Golgi complex. Plays a role in the maturation of phagosomes that engulf
CC pathogens, such as S.aureus and Mycobacterium.
CC -!- SUBUNIT: Interacts with GDI1, GDI2 and CHM; phosphorylation at Thr-80
CC disrupts these interactions. {ECO:0000250|UniProtKB:Q86YS6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, phagosome
CC {ECO:0000250|UniProtKB:Q86YS6}. Cytoplasmic vesicle, phagosome membrane
CC {ECO:0000305}; Lipid-anchor {ECO:0000305}; Cytoplasmic side
CC {ECO:0000305}. Golgi apparatus {ECO:0000250|UniProtKB:Q86YS6}. Golgi
CC apparatus, trans-Golgi network membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}. Golgi apparatus, trans-Golgi network
CC {ECO:0000250|UniProtKB:Q86YS6}. Note=Recruited to phagosomes containing
CC S.aureus or M.tuberculosis. {ECO:0000250|UniProtKB:Q86YS6}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; AY593256; AAT86135.1; -; mRNA.
DR RefSeq; NP_001019502.1; NM_001024331.1.
DR AlphaFoldDB; Q53B90; -.
DR SMR; Q53B90; -.
DR STRING; 10116.ENSRNOP00000013299; -.
DR jPOST; Q53B90; -.
DR PaxDb; Q53B90; -.
DR PRIDE; Q53B90; -.
DR Ensembl; ENSRNOT00000013299; ENSRNOP00000013299; ENSRNOG00000037768.
DR GeneID; 500249; -.
DR KEGG; rno:500249; -.
DR UCSC; RGD:1565300; rat.
DR CTD; 339122; -.
DR RGD; 1565300; Rab43.
DR eggNOG; KOG0084; Eukaryota.
DR GeneTree; ENSGT00940000161980; -.
DR HOGENOM; CLU_041217_23_1_1; -.
DR InParanoid; Q53B90; -.
DR OMA; MFVMETS; -.
DR OrthoDB; 1149105at2759; -.
DR PhylomeDB; Q53B90; -.
DR TreeFam; TF300097; -.
DR Reactome; R-RNO-6811440; Retrograde transport at the Trans-Golgi-Network.
DR Reactome; R-RNO-8873719; RAB geranylgeranylation.
DR PRO; PR:Q53B90; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000037768; Expressed in liver and 18 other tissues.
DR Genevisible; Q53B90; RN.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0045335; C:phagocytic vesicle; ISS:UniProtKB.
DR GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; ISS:UniProtKB.
DR GO; GO:0000045; P:autophagosome assembly; IBA:GO_Central.
DR GO; GO:0071346; P:cellular response to interferon-gamma; ISO:RGD.
DR GO; GO:0007030; P:Golgi organization; ISS:UniProtKB.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0090382; P:phagosome maturation; ISS:UniProtKB.
DR GO; GO:0035526; P:retrograde transport, plasma membrane to Golgi; ISS:UniProtKB.
DR GO; GO:1901998; P:toxin transport; ISO:RGD.
DR GO; GO:0019068; P:virion assembly; ISO:RGD.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 2: Evidence at transcript level;
KW Cytoplasmic vesicle; Golgi apparatus; GTP-binding; Lipoprotein; Membrane;
KW Methylation; Nucleotide-binding; Phosphoprotein; Prenylation;
KW Reference proteome.
FT CHAIN 1..210
FT /note="Ras-related protein Rab-43"
FT /id="PRO_0000244617"
FT MOTIF 45..53
FT /note="Effector region"
FT /evidence="ECO:0000250"
FT BINDING 23..30
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 71..75
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 129..132
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 161..162
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 47
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8CG50"
FT MOD_RES 80
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q86YS6"
FT MOD_RES 210
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 208
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 210
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 210 AA; 23229 MW; 4D7DF08F84AE47A0 CRC64;
MAGPGPGDQD EHYDFLFKLV LVGDASVGKT CVVQRFKTGA FSARQGSTIG VDFTMKTLEI
QGKRVKLQIW DTAGQERFRT ITQSYYRSAN GAILAYDISK RSTFLSVPHW IEDVRKYAGS
NIVQLLIGNK SDLADLREVP LAEAQSLAEH YDILCAIETS AKDSSNVEEA FTRVATELIM
RHGGPMFSEK NTDHIQLDSK DIAESWGCGC
