RAB44_MOUSE
ID RAB44_MOUSE Reviewed; 973 AA.
AC Q8CB87; B9EID0;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2018, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Ras-related protein Rab-44;
GN Name=Rab44;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 243-973.
RC STRAIN=C57BL/6J; TISSUE=Bone;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 243-973.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI39373.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC29479.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; GL456179; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK036563; BAC29479.1; ALT_INIT; mRNA.
DR EMBL; BC139372; AAI39373.1; ALT_INIT; mRNA.
DR CCDS; CCDS37535.2; -.
DR RefSeq; NP_001002786.1; NM_001002786.2.
DR RefSeq; XP_006524654.1; XM_006524591.2.
DR RefSeq; XP_006524660.1; XM_006524597.3.
DR RefSeq; XP_011244831.1; XM_011246529.1.
DR AlphaFoldDB; Q8CB87; -.
DR SMR; Q8CB87; -.
DR IntAct; Q8CB87; 1.
DR STRING; 10090.ENSMUSP00000085253; -.
DR iPTMnet; Q8CB87; -.
DR PhosphoSitePlus; Q8CB87; -.
DR jPOST; Q8CB87; -.
DR PaxDb; Q8CB87; -.
DR PeptideAtlas; Q8CB87; -.
DR PRIDE; Q8CB87; -.
DR ProteomicsDB; 253148; -.
DR ABCD; Q8CB87; 21 sequenced antibodies.
DR DNASU; 442827; -.
DR Ensembl; ENSMUST00000233717; ENSMUSP00000156400; ENSMUSG00000064147.
DR GeneID; 442827; -.
DR UCSC; uc008bsi.1; mouse.
DR CTD; 401258; -.
DR MGI; MGI:3045302; Rab44.
DR VEuPathDB; HostDB:ENSMUSG00000064147; -.
DR eggNOG; KOG0078; Eukaryota.
DR GeneTree; ENSGT00940000160379; -.
DR HOGENOM; CLU_012405_0_0_1; -.
DR InParanoid; Q8CB87; -.
DR OMA; DNDDWDQ; -.
DR OrthoDB; 1184845at2759; -.
DR PhylomeDB; Q8CB87; -.
DR TreeFam; TF313199; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR Reactome; R-MMU-8873719; RAB geranylgeranylation.
DR BioGRID-ORCS; 442827; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Rab44; mouse.
DR PRO; PR:Q8CB87; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q8CB87; protein.
DR Bgee; ENSMUSG00000064147; Expressed in granulocyte and 35 other tissues.
DR ExpressionAtlas; Q8CB87; baseline and differential.
DR Genevisible; Q8CB87; MM.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0002553; P:histamine secretion by mast cell; IMP:MGI.
DR GO; GO:0097279; P:histamine secretion mediated by IgE immunoglobulin; IMP:MGI.
DR GO; GO:0043303; P:mast cell degranulation; IMP:MGI.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Coiled coil; GTP-binding; Lipoprotein; Membrane;
KW Nucleotide-binding; Prenylation; Reference proteome.
FT CHAIN 1..973
FT /note="Ras-related protein Rab-44"
FT /id="PRO_0000333078"
FT DOMAIN 77..111
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 112..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 319..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 421..481
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 493..708
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 724..779
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 219..310
FT /evidence="ECO:0000255"
FT COMPBIAS 112..134
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 445..461
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 517..535
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 668..683
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 749..765
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 792..799
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 840..844
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 898..901
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT LIPID 971
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 972
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 973 AA; 106328 MW; BD06161F72A5D095 CRC64;
MEKGKGVSRK GRKLASSRRR QAREPADGQD APVAAEAESW PSDADAELQG FFQDCGAKER
GFVTREDLAE ARFSFLGGEE PQMIFDWVDV ESRGHLSLEE FSSGLKNVFG SSPGTHRLRT
KRSLPSQRES VTSTLPVPEE ADAEEKEAFL ALIGQMETGH SLSEQAEIWK LWRELRQEEP
QLAGNLEGFL AKMSSRLQEA QADREALAWT LRKRDSDHLY KVRQLYEETE EQIRREKQQL
QAQSDSRGMA LSAHMQEALE AKEQEVQRLA EGQRELEAQL LHLSSTQQEA NRENLQLREA
ERDLAGQLEE VRGQLQVTRG HLDTARTRGK VSWQIEEEPS VPRANKEAPD PQAVPTEEAP
LPELFGNNDN WDQFLSSIEA HSHRTLRLCW SPPPSPSSTS APQTPRIVRQ ISISKISALQ
FSQEPASDPD PGPRGSPEVP PGGAKDGKGV EDPKGQDEQD VSSKQPVDSP DSDARPKGSF
LWSLPGALTA ESGTVEAAFR DQLAFEAEPP PQGLSSSPQS PAGSRKQTQT PDLGDKSLWS
GPDPAKQSLE REVMAEDLKL GLGSQGATAL PEGATEPSLS LESVDQVGPE RPVQDATHLA
RQESHAKGFQ EAPGQVLSLD SLPTHLPQSL EEQLRPEEGN LGERGQQDPG SEASESHGLE
ARSMESPQQD DPLPNTSQPP AETEVPAPGQ MSPPRGSPIL GAGAGLAVGT PETTHTLLTL
AESEAQPGPV SMPVQVESKS GAPQPTEPEA ESRPEDPRTD LQEAERSSSP GDLTAGKPQA
DPDYLYHVVF LGDSNVGKTS FLHLLHHDAF ATGLTATVGV DFRVKNLLVD NKTFALQLWD
TAGQERYHSL TRQLLRKAEG VVLMYDVTSQ ESFTHVRYWL DCLQDAGVEG VAMVLLGNKM
DCEEERQVPT EAGRRLAQEL GVSFGECSAA LGHNILEPMM NLARSLKMQE DRLKASLAEV
THPQSTKRAG CCH
