RAB4A_BOVIN
ID RAB4A_BOVIN Reviewed; 218 AA.
AC Q2TBH7;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2013, sequence version 3.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Ras-related protein Rab-4A;
DE EC=3.6.5.2 {ECO:0000250|UniProtKB:P20338};
GN Name=RAB4A;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Small GTPase which cycles between an active GTP-bound and an
CC inactive GDP-bound state (By similarity). Involved in protein
CC transport. Plays a role in vesicular traffic. Mediates VEGFR2 endosomal
CC trafficking to enhance VEGFR2 signaling (By similarity). Acts as a
CC regulator of platelet alpha-granule release during activation and
CC aggregation of platelets (By similarity).
CC {ECO:0000250|UniProtKB:P20338, ECO:0000250|UniProtKB:P56371}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2;
CC Evidence={ECO:0000250|UniProtKB:P20338};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19670;
CC Evidence={ECO:0000250|UniProtKB:P20338};
CC -!- SUBUNIT: Interacts with RAB11FIP1, RABEP1, ZFYVE20 and RUFY1. Interacts
CC with SGSM1, SGSM2 and SGSM3. Interacts (membrane-bound form) with
CC NDRG1; the interaction involves NDRG1 in vesicular recycling of E-
CC cadherin. Interacts (in GTP-bound form) with GRIPAP1. Interacts with
CC RABEP1 and RBSN (By similarity). Does not interact with HPS4 (By
CC similarity). {ECO:0000250|UniProtKB:P20338,
CC ECO:0000250|UniProtKB:P56371}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:P20338};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:P20338}. Cytoplasm
CC {ECO:0000250|UniProtKB:P20338}. Early endosome membrane
CC {ECO:0000250|UniProtKB:P05714}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P05714}. Recycling endosome membrane
CC {ECO:0000250|UniProtKB:P05714}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P05714}. Note=Generally associated with
CC membranes. Cytoplasmic when phosphorylated by CDK1.
CC {ECO:0000250|UniProtKB:P20338}.
CC -!- PTM: Serotonylation of Gln-72 by TGM2 during activation and aggregation
CC of platelets leads to constitutive activation of GTPase activity.
CC {ECO:0000250|UniProtKB:P56371}.
CC -!- PTM: Phosphorylated by CDK1 kinase during mitosis.
CC {ECO:0000250|UniProtKB:P20338}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-6 is the initiator.
CC {ECO:0000305}.
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DR EMBL; BC110198; AAI10199.1; -; mRNA.
DR RefSeq; NP_001033774.1; NM_001038685.2.
DR AlphaFoldDB; Q2TBH7; -.
DR SMR; Q2TBH7; -.
DR BioGRID; 543454; 2.
DR STRING; 9913.ENSBTAP00000046611; -.
DR PaxDb; Q2TBH7; -.
DR PRIDE; Q2TBH7; -.
DR Ensembl; ENSBTAT00000049768; ENSBTAP00000046611; ENSBTAG00000018857.
DR GeneID; 615849; -.
DR KEGG; bta:615849; -.
DR CTD; 5867; -.
DR VEuPathDB; HostDB:ENSBTAG00000018857; -.
DR VGNC; VGNC:33657; RAB4A.
DR eggNOG; KOG0086; Eukaryota.
DR GeneTree; ENSGT00940000157464; -.
DR HOGENOM; CLU_041217_23_1_1; -.
DR InParanoid; Q2TBH7; -.
DR OMA; STQECGC; -.
DR OrthoDB; 1146851at2759; -.
DR TreeFam; TF300032; -.
DR Proteomes; UP000009136; Chromosome 28.
DR Bgee; ENSBTAG00000018857; Expressed in occipital lobe and 101 other tissues.
DR ExpressionAtlas; Q2TBH7; baseline and differential.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0070062; C:extracellular exosome; IEA:Ensembl.
DR GO; GO:0032593; C:insulin-responsive compartment; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0098837; C:postsynaptic recycling endosome; IBA:GO_Central.
DR GO; GO:0055037; C:recycling endosome; IBA:GO_Central.
DR GO; GO:0055038; C:recycling endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003925; F:G protein activity; IEA:UniProtKB-EC.
DR GO; GO:0019003; F:GDP binding; ISS:UniProtKB.
DR GO; GO:0005525; F:GTP binding; ISS:UniProtKB.
DR GO; GO:0003924; F:GTPase activity; ISS:UniProtKB.
DR GO; GO:0019882; P:antigen processing and presentation; IEA:Ensembl.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0032482; P:Rab protein signal transduction; IEA:InterPro.
DR GO; GO:0030100; P:regulation of endocytosis; IBA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR CDD; cd04113; Rab4; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR041819; Rab4.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Endosome; GTP-binding; Hydrolase; Lipoprotein; Membrane;
KW Methylation; Nucleotide-binding; Phosphoprotein; Prenylation;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..218
FT /note="Ras-related protein Rab-4A"
FT /id="PRO_0000226290"
FT MOTIF 42..50
FT /note="Effector region"
FT /evidence="ECO:0000250"
FT BINDING 20..28
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P61018"
FT BINDING 68..72
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62820"
FT BINDING 126..129
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P61018"
FT BINDING 156..158
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P61018"
FT MOD_RES 72
FT /note="5-glutamyl serotonin"
FT /evidence="ECO:0000250|UniProtKB:P56371"
FT MOD_RES 190
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P20338"
FT MOD_RES 204
FT /note="Phosphoserine; by CDK1"
FT /evidence="ECO:0000250|UniProtKB:P20338"
FT MOD_RES 218
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 216
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 218
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 218 AA; 24393 MW; 6C6C20F553274B5A CRC64;
MSQTAMSETY DFLFKFLVIG NAGTGKSCLL HQFIEKKFKD DSNHTIGVEF GSKIINVGGK
YVKLQIWDTA GQERFRSVTR SYYRGAAGAL LVYDITSRET YNALTNWLTD ARMLASQNIV
IILCGNKKDL DTDREVTFLE ASRFAQENEL MFLETSALTG ENVEEAFVQC ARKILNKIES
GELDPERMGS GIQYGDAALR QLRSPRRAQA PSAQECGC
