RAB4A_DANRE
ID RAB4A_DANRE Reviewed; 213 AA.
AC Q6PHI9;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Ras-related protein Rab-4A;
DE EC=3.6.5.2 {ECO:0000250|UniProtKB:P20338};
GN Name=rab4a;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Small GTPase which cycles between an active GTP-bound and an
CC inactive GDP-bound state (By similarity). Involved in protein
CC transport. Plays a role in vesicular traffic. Mediates VEGFR2 endosomal
CC trafficking to enhance VEGFR2 signaling (By similarity).
CC {ECO:0000250|UniProtKB:P20338, ECO:0000250|UniProtKB:P56371}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2;
CC Evidence={ECO:0000250|UniProtKB:P20338};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19670;
CC Evidence={ECO:0000250|UniProtKB:P20338};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:P20338};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:P20338}. Cytoplasm
CC {ECO:0000250|UniProtKB:P20338}. Early endosome membrane
CC {ECO:0000250|UniProtKB:P05714}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P05714}. Recycling endosome membrane
CC {ECO:0000250|UniProtKB:P05714}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P05714}. Note=Generally associated with
CC membranes. Cytoplasmic when phosphorylated by CDK1.
CC {ECO:0000250|UniProtKB:P20338}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH56535.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; BC056535; AAH56535.1; ALT_INIT; mRNA.
DR RefSeq; NP_001119853.1; NM_001126381.1.
DR AlphaFoldDB; Q6PHI9; -.
DR SMR; Q6PHI9; -.
DR STRING; 7955.ENSDARP00000012097; -.
DR PaxDb; Q6PHI9; -.
DR Ensembl; ENSDART00000004420; ENSDARP00000012097; ENSDARG00000019144.
DR GeneID; 403031; -.
DR KEGG; dre:403031; -.
DR CTD; 5867; -.
DR ZFIN; ZDB-GENE-040525-1; rab4a.
DR eggNOG; KOG0086; Eukaryota.
DR GeneTree; ENSGT00940000157464; -.
DR HOGENOM; CLU_041217_23_1_1; -.
DR InParanoid; Q6PHI9; -.
DR OMA; STQECGC; -.
DR OrthoDB; 1146851at2759; -.
DR PhylomeDB; Q6PHI9; -.
DR TreeFam; TF300032; -.
DR Reactome; R-DRE-1660499; Synthesis of PIPs at the plasma membrane.
DR Reactome; R-DRE-8854214; TBC/RABGAPs.
DR Reactome; R-DRE-8873719; RAB geranylgeranylation.
DR Reactome; R-DRE-8875656; MET receptor recycling.
DR PRO; PR:Q6PHI9; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 13.
DR Bgee; ENSDARG00000019144; Expressed in brain and 23 other tissues.
DR ExpressionAtlas; Q6PHI9; baseline and differential.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0032593; C:insulin-responsive compartment; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0055037; C:recycling endosome; IBA:GO_Central.
DR GO; GO:0055038; C:recycling endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003925; F:G protein activity; IEA:UniProtKB-EC.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0032482; P:Rab protein signal transduction; IEA:InterPro.
DR GO; GO:0030100; P:regulation of endocytosis; IBA:GO_Central.
DR GO; GO:0001944; P:vasculature development; IMP:ZFIN.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR CDD; cd04113; Rab4; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR041819; Rab4.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Endosome; GTP-binding; Hydrolase; Lipoprotein; Membrane;
KW Methylation; Nucleotide-binding; Prenylation; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..213
FT /note="Ras-related protein Rab-4A"
FT /id="PRO_0000121096"
FT MOTIF 37..45
FT /note="Effector region"
FT /evidence="ECO:0000250"
FT BINDING 15..23
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P61018"
FT BINDING 63..67
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62820"
FT BINDING 121..124
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P61018"
FT BINDING 151..153
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P61018"
FT MOD_RES 213
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 211
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 213
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 213 AA; 24002 MW; 26C34E6CCD62C95B CRC64;
MSETYDFLFK FLVIGNAGTG KSCLLHQFIE KRFKDDSNHT IGVEFGSKII SVVNKFVKLQ
IWDTAGQERF RSVTRSYYRG AAGALLVYDI TSRETYNALT NWLTDARMLA SQNIVIILCG
NKKDLDADRE VTFLEASRFA QENELMFLET SALTGENVEE AFVQCARKIL NKIESGELDP
ERMGSGIQYG DAALRQLRSP RRAQAESIQE CGC
