ID   RAB4B_DANRE             Reviewed;         213 AA.
AC   Q68EK7;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Ras-related protein Rab-4B;
DE            EC=3.6.5.2 {ECO:0000250|UniProtKB:P20338};
GN   Name=rab4b; ORFNames=zgc:101015;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Small GTPase which cycles between an active GTP-bound and an
CC       inactive GDP-bound state (By similarity). Protein transport. Probably
CC       involved in vesicular traffic (By similarity).
CC       {ECO:0000250|UniProtKB:P20338, ECO:0000250|UniProtKB:Q91ZR1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2;
CC         Evidence={ECO:0000250|UniProtKB:P20338};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19670;
CC         Evidence={ECO:0000250|UniProtKB:P20338};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC       {ECO:0000305}.
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DR   EMBL; BC080219; AAH80219.1; -; mRNA.
DR   RefSeq; NP_001004002.1; NM_001004002.1.
DR   AlphaFoldDB; Q68EK7; -.
DR   SMR; Q68EK7; -.
DR   STRING; 7955.ENSDARP00000020980; -.
DR   PaxDb; Q68EK7; -.
DR   GeneID; 445498; -.
DR   KEGG; dre:445498; -.
DR   CTD; 53916; -.
DR   ZFIN; ZDB-GENE-040822-15; rab4b.
DR   eggNOG; KOG0086; Eukaryota.
DR   InParanoid; Q68EK7; -.
DR   OrthoDB; 1146851at2759; -.
DR   PhylomeDB; Q68EK7; -.
DR   Reactome; R-DRE-6798695; Neutrophil degranulation.
DR   Reactome; R-DRE-8873719; RAB geranylgeranylation.
DR   Reactome; R-DRE-8875656; MET receptor recycling.
DR   PRO; PR:Q68EK7; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0032593; C:insulin-responsive compartment; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0055037; C:recycling endosome; IBA:GO_Central.
DR   GO; GO:0003925; F:G protein activity; IEA:UniProtKB-EC.
DR   GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR   GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0032482; P:Rab protein signal transduction; IEA:InterPro.
DR   GO; GO:0030100; P:regulation of endocytosis; IBA:GO_Central.
DR   GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR   CDD; cd04113; Rab4; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR041819; Rab4.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR001806; Small_GTPase.
DR   Pfam; PF00071; Ras; 1.
DR   SMART; SM00174; RHO; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51419; RAB; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; GTP-binding; Hydrolase; Lipoprotein; Membrane; Methylation;
KW   Nucleotide-binding; Prenylation; Protein transport; Reference proteome;
KW   Transport.
FT   CHAIN           1..213
FT                   /note="Ras-related protein Rab-4B"
FT                   /id="PRO_0000121102"
FT   MOTIF           37..45
FT                   /note="Effector region"
FT                   /evidence="ECO:0000250"
FT   BINDING         15..23
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:P61018"
FT   BINDING         63..67
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:P62820"
FT   BINDING         121..124
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:P61018"
FT   BINDING         151..153
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:P61018"
FT   MOD_RES         67
FT                   /note="5-glutamyl serotonin"
FT                   /evidence="ECO:0000250|UniProtKB:Q91ZR1"
FT   MOD_RES         213
FT                   /note="Cysteine methyl ester"
FT                   /evidence="ECO:0000250"
FT   LIPID           211
FT                   /note="S-geranylgeranyl cysteine"
FT                   /evidence="ECO:0000250"
FT   LIPID           213
FT                   /note="S-geranylgeranyl cysteine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   213 AA;  23725 MW;  790858A98C93A614 CRC64;
     MSETYDFLFK FLVIGSAGTG KSCLLHQFIE NKFKQDSNHT IGVEFGSRVV NVGGKTVKLQ
     IWDTAGQERF RSVTRSYYRG AAGALLVYDI TSRETYNALT NWLTDARTLA SPNIVIILCG
     NKKDLDADRE VTFLEASRFA QENELMFLET SALTGENVEE AFLKCARSIP NKIESGELDP
     ERMGSGIQYG DASLRQIRQP RGSAAQTKQQ CNC