RAB8A_DICDI
ID RAB8A_DICDI Reviewed; 208 AA.
AC P20790; Q54VH5;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Ras-related protein Rab-8A;
DE AltName: Full=GTP-binding protein SAS1;
GN Name=rab8A; Synonyms=sas1, sasA; ORFNames=DDB_G0280043;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2109188; DOI=10.1128/mcb.10.5.2367-2378.1990;
RA Saxe S.A., Kimmel A.R.;
RT "SAS1 and SAS2, GTP-binding protein genes in Dictyostelium discoideum with
RT sequence similarities to essential genes in Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 10:2367-2378(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3149563; DOI=10.1002/dvg.1020090408;
RA Saxe S.A., Kimmel A.R.;
RT "Genes encoding novel GTP-binding proteins in Dictyostelium.";
RL Dev. Genet. 9:259-265(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Protein transport. Probably involved in vesicular traffic (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; M34457; AAA33249.1; -; Genomic_DNA.
DR EMBL; AAFI02000035; EAL67248.1; -; Genomic_DNA.
DR PIR; A34716; A34716.
DR RefSeq; XP_641380.1; XM_636288.1.
DR AlphaFoldDB; P20790; -.
DR SMR; P20790; -.
DR IntAct; P20790; 1.
DR MINT; P20790; -.
DR STRING; 44689.DDB0214885; -.
DR PaxDb; P20790; -.
DR EnsemblProtists; EAL67248; EAL67248; DDB_G0280043.
DR GeneID; 8622514; -.
DR KEGG; ddi:DDB_G0280043; -.
DR dictyBase; DDB_G0280043; rab8A.
DR eggNOG; KOG0078; Eukaryota.
DR HOGENOM; CLU_041217_23_1_1; -.
DR InParanoid; P20790; -.
DR OMA; ENFFLAW; -.
DR PhylomeDB; P20790; -.
DR Reactome; R-DDI-210500; Glutamate Neurotransmitter Release Cycle.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR Reactome; R-DDI-8873719; RAB geranylgeranylation.
DR PRO; PR:P20790; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0031164; C:contractile vacuolar membrane; IDA:dictyBase.
DR GO; GO:0000331; C:contractile vacuole; IDA:dictyBase.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0005811; C:lipid droplet; HDA:dictyBase.
DR GO; GO:0140220; C:pathogen-containing vacuole; IDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; ISS:dictyBase.
DR GO; GO:0032794; F:GTPase activating protein binding; IPI:dictyBase.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0044877; F:protein-containing complex binding; IDA:dictyBase.
DR GO; GO:0030036; P:actin cytoskeleton organization; IMP:dictyBase.
DR GO; GO:0016339; P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules; IMP:dictyBase.
DR GO; GO:0070177; P:contractile vacuole discharge; IMP:dictyBase.
DR GO; GO:0033298; P:contractile vacuole organization; IGI:dictyBase.
DR GO; GO:0140025; P:contractile vacuole tethering involved in discharge; IMP:dictyBase.
DR GO; GO:0009306; P:protein secretion; IBA:GO_Central.
DR GO; GO:0031157; P:regulation of aggregate size involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0051600; P:regulation of endocytosis by exocyst localization; IMP:dictyBase.
DR GO; GO:0017157; P:regulation of exocytosis; IBA:GO_Central.
DR GO; GO:0050708; P:regulation of protein secretion; IMP:dictyBase.
DR GO; GO:0006904; P:vesicle docking involved in exocytosis; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 3: Inferred from homology;
KW Cell membrane; GTP-binding; Lipoprotein; Membrane; Nucleotide-binding;
KW Prenylation; Protein transport; Reference proteome; Transport.
FT CHAIN 1..208
FT /note="Ras-related protein Rab-8A"
FT /id="PRO_0000121275"
FT REGION 186..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 186..202
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 22..29
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 70..74
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 128..131
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT LIPID 207
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 208
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 208 AA; 23244 MW; BF681EF5C810019E CRC64;
MTSPATNKSA AYDYLIKLLL IGDSGVGKSC LLLRFSEDSF TPSFITTIGI DFKIRTIELE
GKRIKLQIWD TAGQERFRTI TTAYYRGAMG ILLVYDVTDE KSFGNIRNWI RNIEQHATDS
VNKMLIGNKC DMAEKKVVDS SRGKSLADEY GIKFLETSAK NSINVEEAFI SLAKDIKKRM
IDTPNEQPQV VQPGTNLGAN NNKKKACC
