RABEK_MOUSE
ID RABEK_MOUSE Reviewed; 380 AA.
AC Q8VCH5; Q8BZG7;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Rab9 effector protein with kelch motifs {ECO:0000305};
GN Name=Rabepk {ECO:0000312|MGI:MGI:2139530};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH PIKFYVE.
RX PubMed=14530284; DOI=10.1074/jbc.m307260200;
RA Ikonomov O.C., Sbrissa D., Mlak K., Deeb R., Fligger J., Soans A.,
RA Finley R.L. Jr., Shisheva A.;
RT "Active PIKfyve associates with and promotes the membrane attachment of the
RT late endosome-to-trans-Golgi network transport factor Rab9 effector p40.";
RL J. Biol. Chem. 278:50863-50871(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Rab9 effector required for endosome to trans-Golgi network
CC (TGN) transport. {ECO:0000250|UniProtKB:Q7Z6M1}.
CC -!- SUBUNIT: Interacts with PIKFYVE; the interaction recruits RABEPK to the
CC endosomal membrane (PubMed:14530284). Interacts with RAB9 in its GTP-
CC bound conformation (By similarity). {ECO:0000250|UniProtKB:Q7Z6M1,
CC ECO:0000269|PubMed:14530284}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q7Z6M1}.
CC Endosome membrane. Note=Interaction with PIKFYVE and subsequent
CC phosphorylation recruits it to the endosomal membrane.
CC {ECO:0000250|UniProtKB:Q7Z6M1}.
CC -!- PTM: Phosphorylated on Ser residues by PIKFYVE.
CC {ECO:0000250|UniProtKB:Q7Z6M1}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-9 is the initiator.
CC {ECO:0000305}.
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DR EMBL; AK035343; BAC29041.1; -; mRNA.
DR EMBL; AL929106; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC019800; AAH19800.2; -; mRNA.
DR CCDS; CCDS15951.1; -.
DR RefSeq; NP_663497.2; NM_145522.4.
DR AlphaFoldDB; Q8VCH5; -.
DR SMR; Q8VCH5; -.
DR BioGRID; 230682; 5.
DR STRING; 10090.ENSMUSP00000122360; -.
DR iPTMnet; Q8VCH5; -.
DR PhosphoSitePlus; Q8VCH5; -.
DR EPD; Q8VCH5; -.
DR MaxQB; Q8VCH5; -.
DR PaxDb; Q8VCH5; -.
DR PRIDE; Q8VCH5; -.
DR ProteomicsDB; 300292; -.
DR Antibodypedia; 16386; 229 antibodies from 27 providers.
DR DNASU; 227746; -.
DR Ensembl; ENSMUST00000145903; ENSMUSP00000122360; ENSMUSG00000070953.
DR GeneID; 227746; -.
DR KEGG; mmu:227746; -.
DR UCSC; uc008jiu.2; mouse.
DR CTD; 10244; -.
DR MGI; MGI:2139530; Rabepk.
DR VEuPathDB; HostDB:ENSMUSG00000070953; -.
DR eggNOG; KOG0379; Eukaryota.
DR GeneTree; ENSGT00940000158763; -.
DR InParanoid; Q8VCH5; -.
DR OMA; HEWDIPE; -.
DR OrthoDB; 933937at2759; -.
DR PhylomeDB; Q8VCH5; -.
DR TreeFam; TF329153; -.
DR Reactome; R-MMU-6811440; Retrograde transport at the Trans-Golgi-Network.
DR BioGRID-ORCS; 227746; 2 hits in 61 CRISPR screens.
DR ChiTaRS; Rabepk; mouse.
DR PRO; PR:Q8VCH5; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8VCH5; protein.
DR Bgee; ENSMUSG00000070953; Expressed in spermatid and 246 other tissues.
DR ExpressionAtlas; Q8VCH5; baseline and differential.
DR Genevisible; Q8VCH5; MM.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 2.120.10.80; -; 2.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR011498; Kelch_2.
DR Pfam; PF01344; Kelch_1; 1.
DR Pfam; PF07646; Kelch_2; 1.
DR SUPFAM; SSF117281; SSF117281; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Endosome; Kelch repeat; Membrane; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..380
FT /note="Rab9 effector protein with kelch motifs"
FT /id="PRO_0000280617"
FT REPEAT 57..103
FT /note="Kelch 1"
FT REPEAT 108..154
FT /note="Kelch 2"
FT REPEAT 159..211
FT /note="Kelch 3"
FT REPEAT 212..258
FT /note="Kelch 4"
FT REPEAT 262..311
FT /note="Kelch 5"
FT REPEAT 357..380
FT /note="Kelch 6"
SQ SEQUENCE 380 AA; 41119 MW; FC7FD5CC44BAD21A CRC64;
MRGLRQGIMK QLPILEPGDK PRKATWYTLT CPGDRPCPRV GHSCSYFPPV GDAESGKIFI
VGGANPNQSF SDVHTMDLGT HQWDTATREG LLPRYEHASF LPSCSPHSIW VFGGADQSGN
RNCLQVMSPE DRTWSTPEVT GSPPSPRTFH TSSAAIGNQL YVFGGGERGA QPVEDVKLHV
FDANTLTWSQ PETHGSPPSP RHGHVMVAAG TKLFIHGGLA GDKFFDDLHC IDIGDMSWQK
LGPTGAVPVG CAAHAAVAVG HHVYMFGGMT ATGALNMMYK YHTEKQHWTV LQFDTSLPAG
RLDHSMCVIP WPVMSTSENK DSDSVILTLQ DEKGDAAEKA ETQSGGPHEE SPTTVLLCFV
FGGMNTEGEV YDDCLVTVVD
