RACA_DICDI
ID RACA_DICDI Reviewed; 598 AA.
AC P34147; Q54LK0; Q9GPT3;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Rho-related protein racA;
GN Name=racA; ORFNames=DDB_G0286555;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=11222756; DOI=10.1093/nar/29.5.1068;
RA Rivero F., Dislich H., Gloeckner G., Noegel A.A.;
RT "The Dictyostelium discoideum family of Rho-related proteins.";
RL Nucleic Acids Res. 29:1068-1079(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 11-179.
RC STRAIN=AX3;
RX PubMed=8294042; DOI=10.1016/0378-1119(93)90448-c;
RA Bush J.M. IV, Franek K., Cardelli J.A.;
RT "Cloning and characterization of seven novel Dictyostelium discoideum rac-
RT related genes belonging to the rho family of GTPases.";
RL Gene 136:61-68(1993).
RN [4]
RP INTERACTION WITH PAKB.
RX PubMed=15509655; DOI=10.1091/mbc.e04-06-0534;
RA de la Roche M., Mahasneh A., Lee S.-F., Rivero F., Cote G.P.;
RT "Cellular distribution and functions of wild-type and constitutively
RT activated Dictyostelium PakB.";
RL Mol. Biol. Cell 16:238-247(2005).
CC -!- SUBUNIT: Interacts with pakB. {ECO:0000269|PubMed:15509655}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the small GTPase
CC superfamily. Rho family. {ECO:0000305}.
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DR EMBL; L11591; AAC37387.1; -; mRNA.
DR EMBL; AAFI02000089; EAL64033.1; -; Genomic_DNA.
DR EMBL; AF310886; AAG45115.1; -; Genomic_DNA.
DR RefSeq; XP_637561.1; XM_632469.1.
DR AlphaFoldDB; P34147; -.
DR SMR; P34147; -.
DR IntAct; P34147; 1.
DR STRING; 44689.DDB0191173; -.
DR PaxDb; P34147; -.
DR EnsemblProtists; EAL64033; EAL64033; DDB_G0286555.
DR GeneID; 8625701; -.
DR KEGG; ddi:DDB_G0286555; -.
DR dictyBase; DDB_G0286555; racA.
DR eggNOG; KOG0393; Eukaryota.
DR HOGENOM; CLU_029897_0_0_1; -.
DR InParanoid; P34147; -.
DR OMA; CLCFTGG; -.
DR PhylomeDB; P34147; -.
DR Reactome; R-DDI-9013423; RAC3 GTPase cycle.
DR PRO; PR:P34147; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005938; C:cell cortex; IBA:GO_Central.
DR GO; GO:0042995; C:cell projection; IBA:GO_Central.
DR GO; GO:0031410; C:cytoplasmic vesicle; IBA:GO_Central.
DR GO; GO:0005856; C:cytoskeleton; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; IPI:dictyBase.
DR GO; GO:0007015; P:actin filament organization; IBA:GO_Central.
DR GO; GO:0030865; P:cortical cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0007163; P:establishment or maintenance of cell polarity; IBA:GO_Central.
DR GO; GO:0032956; P:regulation of actin cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0008360; P:regulation of cell shape; IBA:GO_Central.
DR GO; GO:0009617; P:response to bacterium; HEP:dictyBase.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR Gene3D; 3.30.710.10; -; 2.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR003578; Small_GTPase_Rho.
DR PANTHER; PTHR24072; PTHR24072; 1.
DR Pfam; PF00651; BTB; 2.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00225; BTB; 2.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54695; SSF54695; 2.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS50097; BTB; 2.
DR PROSITE; PS51420; RHO; 1.
PE 1: Evidence at protein level;
KW GTP-binding; Nucleotide-binding; Reference proteome; Repeat.
FT CHAIN 1..598
FT /note="Rho-related protein racA"
FT /id="PRO_0000198898"
FT DOMAIN 239..344
FT /note="BTB 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 405..472
FT /note="BTB 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT REGION 175..210
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 32..40
FT /note="Effector region"
FT /evidence="ECO:0000255"
FT COMPBIAS 175..202
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 11..17
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 57..61
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 115..118
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 598 AA; 67402 MW; A98C602599246267 CRC64;
MQAIKLVVVG DGAVGKSCLL IAYTTNAFPG EYVPTVFDNY SANVMIDGKP FNLGLWDTAG
QEDYDRLRPL SYPQTDVFLI CFSIVSRASF ENIRAKWYPE ILHHAPNIPI VLVGTKNDLR
GHHDLKRPEV SAAEANNLVR ELGFSGYVET SALLQTNLRE LFSLAIRTAT SPKSASAKKK
GGFFSSSSSS SSSSSSKSSE KSVPIPPVMP PAGKAPWIDI ITSSYDKEMR ETLNSESFSD
VKFTFFEERP VYAHRVILCS ASEFFRRVFG YTLSSDQAEF KLDDLNEGKV KNIRSVTIKP
NEKNDDGTKS SGEFVEISID SSINRRVFNR FIEFLYTGVL NYLDKNDQLQ ETIALAESVG
FKYLASACKN VLGGNEDLNP SIGTFLNDQL GETSKELFFT KKILSDIQFI VEGRSIFAHK
ALIFSRSNVV NALIGRNFIE REGKVEMSDD VTFDSFMAIL EYLYTAHAPI EEGDSVGILV
LGNRFDLRRL VSLCELYISK EIERATTIGI FRSELDIIGL LICAQRHNAP QLEKFCLHFI
SSNYQPMRRR KEFALLDDKT KKHIEDNQWP PIHYLRAVED YEKEMEKLKS SSKGWFKK
