ID   RAD1_LOTJA              Reviewed;         509 AA.
AC   A0A1B1WAJ0;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2016, sequence version 1.
DT   25-MAY-2022, entry version 17.
DE   RecName: Full=GRAS family protein RAD1 {ECO:0000305};
DE   AltName: Full=Protein REQUIRED FOR ARBUSCULE DEVELOPMENT 1 {ECO:0000303|PubMed:25560877};
DE            Short=LjRAD1 {ECO:0000303|PubMed:25560877};
GN   Name=RAD1 {ECO:0000303|PubMed:25560877};
OS   Lotus japonicus (Lotus corniculatus var. japonicus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; robinioid clade; Loteae; Lotus.
OX   NCBI_TaxID=34305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE
RP   SPECIFICITY, INDUCTION BY RHIZOPHAGUS IRREGULARIS, INTERACTION WITH RAM1
RP   AND NSP2, AND SUBCELLULAR LOCATION.
RC   STRAIN=cv. Gifu / B-129;
RX   PubMed=25560877; DOI=10.1104/pp.114.255430;
RA   Xue L., Cui H., Buer B., Vijayakumar V., Delaux P.-M., Junkermann S.,
RA   Bucher M.;
RT   "Network of GRAS transcription factors involved in the control of arbuscule
RT   development in Lotus japonicus.";
RL   Plant Physiol. 167:854-871(2015).
CC   -!- FUNCTION: Transcription factor acting as a regulator of arbuscular
CC       mycorrhiza (AM)-related genes (e.g. PT4, STR and RAM2)
CC       (PubMed:25560877). Required for the morphogenesis of arbuscules upon
CC       symbiosis with AM fungi (e.g. Rhizophagus irregularis)
CC       (PubMed:25560877). Also involved in restricting mycorrhizal
CC       colonization of the root meristem (PubMed:25560877).
CC       {ECO:0000269|PubMed:25560877}.
CC   -!- SUBUNIT: Interacts with RAM1 and NSP2. {ECO:0000269|PubMed:25560877}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25560877}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots under low phosphate (Pi)
CC       conditions. {ECO:0000269|PubMed:25560877}.
CC   -!- DEVELOPMENTAL STAGE: Accumulates in well-developed arbuscule-containing
CC       cells during arbuscule development upon arbuscular mycorrhizal (AM)
CC       symbiosis. {ECO:0000269|PubMed:25560877}.
CC   -!- INDUCTION: Induced in root epidermal cells and outer cortical cells
CC       upon root colonization by arbuscular mycorrhiza (AM) fungi (e.g.
CC       Rhizophagus irregularis), especially in arbuscule-containing cells
CC       under low phosphate (Pi) conditions. {ECO:0000269|PubMed:25560877}.
CC   -!- DISRUPTION PHENOTYPE: Strongly reduced number of arbuscules undergoing
CC       accelerated degeneration during root colonization by arbuscular
CC       mycorrhiza (AM) fungi (e.g. Rhizophagus irregularis).
CC       {ECO:0000269|PubMed:25560877}.
CC   -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01191}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; KU563537; ANW62077.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1B1WAJ0; -.
DR   SMR; A0A1B1WAJ0; -.
DR   OMA; ENGEGMK; -.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0036377; P:arbuscular mycorrhizal association; IMP:UniProtKB.
DR   GO; GO:0016036; P:cellular response to phosphate starvation; IEP:UniProtKB.
DR   GO; GO:0010247; P:detection of phosphate ion; IEP:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR   InterPro; IPR005202; TF_GRAS.
DR   PANTHER; PTHR31636; PTHR31636; 1.
DR   Pfam; PF03514; GRAS; 1.
DR   PROSITE; PS50985; GRAS; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Transcription; Transcription regulation.
FT   CHAIN           1..509
FT                   /note="GRAS family protein RAD1"
FT                   /id="PRO_0000450025"
FT   DOMAIN          125..508
FT                   /note="GRAS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          132..198
FT                   /note="Leucine repeat I (LRI)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          217..286
FT                   /note="VHIID"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          299..331
FT                   /note="Leucine repeat II (LRII)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          340..431
FT                   /note="PFYRE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          434..508
FT                   /note="SAW"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   MOTIF           248..252
FT                   /note="VHIID"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
SQ   SEQUENCE   509 AA;  56700 MW;  6BF1CE5724604054 CRC64;
     MSPPLYSVLL EDENSVFLLD LDLSSPMGFH AYPHLPILDS SIANWSLPFS ISDETFRESK
     KLKRTMIPIS SADFSISSSS SLSVSVNSIP RLNFRDHIRT YKRYLAAEEL PEDTNSSESV
     VGAEEDGCAD GMRLVQLLIA CAEAVACRDK AHASMLLSEL KSNALVFGSS FQRVASCFVQ
     GLAERLTLIQ PIGSGAGVSQ SMMNIMDAAS EEMEEAYRLV YETCPHIQFG HFVANSTILE
     AFEGESFVHV VDLGMSLGLP HGHQWRGLIH SLANRASGHG RVRRLRITAI GLCIARLQAI
     GDELSDYANN LGINLEFSVV QKNLENLQPE DIKVNDDEAL VVNSILQLHC VVKESRGALN
     SVLQMIHGLS PKVLVMVEQD SSHNGPFFLG RFMESLHYYS AIFDSLDAML PKYDTKRAKM
     EQFYFAEEIK NIVSCEGPLR MERHERVDQW RRRMSRAGFQ AAPIKMVAQA KQWLLKNKIC
     DGYTVVEEKG CLVLGWKSKP IVAASCWKC