RAD1_MEDTR
ID RAD1_MEDTR Reviewed; 521 AA.
AC G7JMM0;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 1.
DT 25-MAY-2022, entry version 45.
DE RecName: Full=GRAS family protein RAD1 {ECO:0000305};
DE AltName: Full=Protein REQUIRED FOR ARBUSCULE DEVELOPMENT 1 {ECO:0000303|PubMed:26511916};
DE Short=MtRAD1 {ECO:0000303|PubMed:26511916};
GN Name=RAD1 {ECO:0000303|PubMed:26511916};
GN OrderedLocusNames=MTR_4g104020 {ECO:0000312|EMBL:AES91080.1};
GN ORFNames=MtrunA17_Chr4g0057891 {ECO:0000312|EMBL:RHN63401.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY RAM1 AND GLOMUS VERSIFORME,
RP AND INTERACTION WITH RAM1.
RX PubMed=26511916; DOI=10.1104/pp.15.01155;
RA Park H.-J., Floss D.S., Levesque-Tremblay V., Bravo A., Harrison M.J.;
RT "Hyphal branching during arbuscule development requires reduced arbuscular
RT mycorrhiza1.";
RL Plant Physiol. 169:2774-2788(2015).
CC -!- FUNCTION: Transcription factor acting as a regulator of arbuscular
CC mycorrhiza (AM)-related genes (e.g. STR) (PubMed:26511916). Required
CC for the morphogenesis of arbuscules upon symbiosis with AM fungi (e.g.
CC Glomus versiforme) (PubMed:26511916). Also involved in restricting
CC mycorrhizal colonization of the root meristem (By similarity).
CC {ECO:0000250|UniProtKB:A0A1B1WAJ0, ECO:0000269|PubMed:26511916}.
CC -!- SUBUNIT: Interacts with RAM1 (PubMed:26511916). Interacts with NSP2 (By
CC similarity). {ECO:0000250|UniProtKB:A0A1B1WAJ0,
CC ECO:0000269|PubMed:26511916}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:G7L166}.
CC -!- INDUCTION: Accumulates in roots, in a RAM1-dependent manner, during
CC colonization by arbuscular mycorrhizal fungi (e.g. Glomus versiforme)
CC (PubMed:26511916). Triggered by RAM1 (PubMed:26511916).
CC {ECO:0000269|PubMed:26511916}.
CC -!- DISRUPTION PHENOTYPE: Reduced intraradical colonization by arbuscular
CC mycorrhiza (AM)-fungi (e.g. Glomus versiforme) upon symbiosis in roots.
CC {ECO:0000269|PubMed:26511916}.
CC -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000255|PROSITE-
CC ProRule:PRU01191}.
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DR EMBL; CM001220; AES91080.1; -; Genomic_DNA.
DR EMBL; PSQE01000004; RHN63401.1; -; Genomic_DNA.
DR RefSeq; XP_003608883.1; XM_003608835.1.
DR AlphaFoldDB; G7JMM0; -.
DR SMR; G7JMM0; -.
DR STRING; 3880.AES91080; -.
DR EnsemblPlants; AES91080; AES91080; MTR_4g104020.
DR GeneID; 11413579; -.
DR Gramene; AES91080; AES91080; MTR_4g104020.
DR KEGG; mtr:MTR_4g104020; -.
DR eggNOG; ENOG502QRCE; Eukaryota.
DR HOGENOM; CLU_011924_0_6_1; -.
DR OMA; ENGEGMK; -.
DR OrthoDB; 577001at2759; -.
DR Proteomes; UP000002051; Chromosome 4.
DR Proteomes; UP000265566; Chromosome 4.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..521
FT /note="GRAS family protein RAD1"
FT /id="PRO_0000450024"
FT DOMAIN 137..520
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 144..212
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 231..301
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 311..343
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 352..443
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 446..520
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 262..266
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
SQ SEQUENCE 521 AA; 58794 MW; DA479BB8AAD62805 CRC64;
MSPALYASTF KCEVDENPSL MGSYYASLYP NLPILENSAT STWILNPFSD HETETIRDHK
KLKRSTVTIP IWFANFSSHS NSFFNNINSN NNSVNSIPRL HFRDHIRTYK QRYFASEAVE
EAAEDNFNYN NCEAEEDGSC ADGMRLVQLL IACAEAVACR DKSHASVLLS ELKSNALVFG
SSFQRVASCF VQGLTERLTL IQPIGNNSAG SDTKSMMNIM DAASEEMEEA FKLVYENCPH
IQFGHFVANS IILEAFEGES FLHVVDLGMS LGLPHGHQWR GLIQSLADRS SHRVRRLRIT
AIGLCIARIQ VIGEELSIYA KNLGIHLEFS IVEKNLENLK PKDIKVNEKE VLVVNSILQL
HCVVKESRGA LNAVLQMIHG LSPKVLVMAE QDSGHNGPFF LGRFMESLHY YSAIFDSLDA
MLPKYDTKRA KMEQFYFAEE IKNIVSCEGP LRMERHEKVD QWRRRMSRAG FQGSPIKMVV
QAKQWLVKNN VCDGYTVVEE KGCLVLGWKS KPIVAVSCWK C
