ID   RAD1_PONAB              Reviewed;         282 AA.
AC   Q5R7X9;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Cell cycle checkpoint protein RAD1;
DE            EC=3.1.11.2;
DE   AltName: Full=DNA repair exonuclease rad1 homolog;
GN   Name=RAD1;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the 9-1-1 cell-cycle checkpoint response complex
CC       that plays a major role in DNA repair. The 9-1-1 complex is recruited
CC       to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp
CC       loader complex. Acts then as a sliding clamp platform on DNA for
CC       several proteins involved in long-patch base excision repair (LP-BER).
CC       The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by
CC       increasing its affinity for the 3'-OH end of the primer-template and
CC       stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1
CC       cleavage activity on substrates with double, nick, or gap flaps of
CC       distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch
CC       base excision repair substrates. The 9-1-1 complex is necessary for the
CC       recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring
CC       during the S phase. Possesses 3'->5' double-stranded DNA exonuclease
CC       activity (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.11.2;
CC   -!- SUBUNIT: Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex,
CC       composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with
CC       LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex
CC       associates with the RAD17-RFC complex. RAD1 interacts with POLB, FEN1,
CC       HUS1, HUS1B, RAD9A and RAD9B. Interacts with DNAJC7 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the rad1 family. {ECO:0000305}.
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DR   EMBL; CR859979; CAH92131.1; -; mRNA.
DR   RefSeq; NP_001126246.1; NM_001132774.1.
DR   RefSeq; XP_009238895.1; XM_009240620.1.
DR   AlphaFoldDB; Q5R7X9; -.
DR   SMR; Q5R7X9; -.
DR   STRING; 9601.ENSPPYP00000017189; -.
DR   Ensembl; ENSPPYT00000053099; ENSPPYP00000027894; ENSPPYG00000031706.
DR   GeneID; 100173217; -.
DR   KEGG; pon:100173217; -.
DR   CTD; 5810; -.
DR   eggNOG; KOG3194; Eukaryota.
DR   GeneTree; ENSGT00500000044913; -.
DR   HOGENOM; CLU_035332_2_1_1; -.
DR   InParanoid; Q5R7X9; -.
DR   OMA; QITMSPE; -.
DR   OrthoDB; 1440961at2759; -.
DR   TreeFam; TF101211; -.
DR   Proteomes; UP000001595; Chromosome 5.
DR   GO; GO:0005694; C:chromosome; IEA:Ensembl.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:Ensembl.
DR   GO; GO:0008853; F:exodeoxyribonuclease III activity; IEA:UniProtKB-EC.
DR   GO; GO:0071479; P:cellular response to ionizing radiation; IEA:Ensembl.
DR   GO; GO:0000077; P:DNA damage checkpoint signaling; IEA:Ensembl.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0051598; P:meiotic recombination checkpoint signaling; IEA:Ensembl.
DR   InterPro; IPR003011; Cell_cycle_checkpoint_Rad1.
DR   InterPro; IPR003021; Rad1_Rec1_Rad17.
DR   PANTHER; PTHR10870; PTHR10870; 1.
DR   Pfam; PF02144; Rad1; 1.
DR   PRINTS; PR01245; RAD1REC1.
DR   PRINTS; PR01246; RAD1REPAIR.
PE   2: Evidence at transcript level;
KW   DNA damage; DNA repair; Exonuclease; Hydrolase; Nuclease; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..282
FT                   /note="Cell cycle checkpoint protein RAD1"
FT                   /id="PRO_0000225007"
SQ   SEQUENCE   282 AA;  31827 MW;  075FBD4CF8A4FDB2 CRC64;
     MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ
     ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM
     LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS EGLREAFSEL DMTSEVLQIT
     MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK
     VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES