ID   RAD25_HALMA             Reviewed;         621 AA.
AC   Q5V5F7;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   14-MAY-2014, sequence version 2.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Putative DNA helicase Rad25 {ECO:0000255|HAMAP-Rule:MF_01489};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01489};
GN   Name=rad25 {ECO:0000255|HAMAP-Rule:MF_01489}; OrderedLocusNames=rrnAC0181;
OS   Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM
OS   B-1809) (Halobacterium marismortui).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Haloarculaceae; Haloarcula.
OX   NCBI_TaxID=272569;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809;
RX   PubMed=15520287; DOI=10.1101/gr.2700304;
RA   Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W.,
RA   Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E.,
RA   Hood L., Ng W.V.;
RT   "Genome sequence of Haloarcula marismortui: a halophilic archaeon from the
RT   Dead Sea.";
RL   Genome Res. 14:2221-2234(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01489};
CC   -!- SIMILARITY: Belongs to the helicase family. RAD25/XPB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01489}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAV45245.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AY596297; AAV45245.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_004963031.1; NZ_CP039138.1.
DR   AlphaFoldDB; Q5V5F7; -.
DR   SMR; Q5V5F7; -.
DR   STRING; 272569.rrnAC0181; -.
DR   EnsemblBacteria; AAV45245; AAV45245; rrnAC0181.
DR   GeneID; 40154481; -.
DR   GeneID; 64823064; -.
DR   KEGG; hma:rrnAC0181; -.
DR   PATRIC; fig|272569.17.peg.977; -.
DR   eggNOG; arCOG00874; Archaea.
DR   HOGENOM; CLU_008213_4_0_2; -.
DR   Proteomes; UP000001169; Chromosome I.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_01489; Helicase_Rad25_arch; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR032438; ERCC3_RAD25_C.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR030882; Helicase_Rad25_arc.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF16203; ERCC3_RAD25_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..621
FT                   /note="Putative DNA helicase Rad25"
FT                   /id="PRO_0000429043"
FT   DOMAIN          268..417
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01489"
FT   DOMAIN          469..621
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01489"
FT   REGION          441..465
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           371..374
FT                   /note="DEAH box"
FT   COMPBIAS        446..462
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         281..288
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01489"
SQ   SEQUENCE   621 AA;  69875 MW;  B2F4A5192289CDA3 CRC64;
     MTDEEPADRD DDSDSTPELE LDAVYDAIDA VGRPHLTATE FSRKTDLTPD EARAALERLA
     DDGDIERQDV SEVESVWYPT DIAEVTDRER VVLFPDRREV VVEHPDQFTR AQLSQFARLQ
     DTNRSGGYVY ELREEDIWAA PHESLDDLLT TMRDVLGERS PHLEEWVTSQ WERARKFRLK
     THEDGYVVLE AESDDLMGNV ARPKLDDDHL RAPISDSESW VNEDATAEIK RTLYEAGYPV
     RDDRDLETGD AIEMDLRLRL RDYQQDWVER FTEQGSGVFV GPPGSGKTVA AMGAMAAIGG
     ETLILVPSRE LATQWRDELV RHTSLTDDDI GEYHGGEKEI RAVTIATYRT AGMDRHRKLF
     DQRKWGLIVF DEVHHVPSPI HRRSADLQTK HRLGLTATPT RESDDEEEIF TLIGPPIGTD
     WGKLFDEGYV AEPEVEIRLV PWGDETEQSE YSSTSGHDRR QAAASNTGKI DEIRYALAEN
     PAAKALVFIE YLDQGEAISE AIDAPFISGE TPHARREKLF DEFRRGELTT LVVSRVGDEG
     IDLPDAELAL VASGLGGSRR QGAQRAGRTM RPAGDARMVI LATRGTTEED FVRRQMRHLA
     SKGIRVTETE AEAVEPPAKT E