ID   RAD25_HALSA             Reviewed;         600 AA.
AC   Q9HPZ2;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   14-MAY-2014, sequence version 2.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Putative DNA helicase Rad25 {ECO:0000255|HAMAP-Rule:MF_01489};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01489};
GN   Name=rad25 {ECO:0000255|HAMAP-Rule:MF_01489}; Synonyms=rhl;
GN   OrderedLocusNames=VNG_1406G;
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=64091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01489};
CC   -!- SIMILARITY: Belongs to the helicase family. RAD25/XPB subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01489}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG19725.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE004437; AAG19725.1; ALT_INIT; Genomic_DNA.
DR   PIR; A84295; A84295.
DR   RefSeq; WP_012289321.1; NC_002607.1.
DR   AlphaFoldDB; Q9HPZ2; -.
DR   SMR; Q9HPZ2; -.
DR   STRING; 64091.VNG_1406G; -.
DR   PaxDb; Q9HPZ2; -.
DR   EnsemblBacteria; AAG19725; AAG19725; VNG_1406G.
DR   GeneID; 5952958; -.
DR   KEGG; hal:VNG_1406G; -.
DR   PATRIC; fig|64091.14.peg.1074; -.
DR   HOGENOM; CLU_074056_0_0_2; -.
DR   InParanoid; Q9HPZ2; -.
DR   OrthoDB; 4838at2157; -.
DR   PhylomeDB; Q9HPZ2; -.
DR   Proteomes; UP000000554; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_01489; Helicase_Rad25_arch; 1.
DR   InterPro; IPR032438; ERCC3_RAD25_C.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR030882; Helicase_Rad25_arc.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF16203; ERCC3_RAD25_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..600
FT                   /note="Putative DNA helicase Rad25"
FT                   /id="PRO_0000429044"
FT   DOMAIN          253..402
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01489"
FT   DOMAIN          457..600
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01489"
FT   REGION          569..600
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           356..359
FT                   /note="DEAH box"
FT   COMPBIAS        569..589
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         266..273
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01489"
SQ   SEQUENCE   600 AA;  66902 MW;  A389DA9E8B29B0C0 CRC64;
     MTDISKDRFL DALEGVGRPV ATAEQIAREL DCTQATAADA LAALAADDEV ERANVEPDPV
     VWYPRDWLEL TDRERIVPFP DRREIIVDQP SQLTRAQLSR VAHLTDTTRT GSYRYEVRAE
     DVWAAPFDSL SDLVAAITEL LPRDCPTLIE WVREQWTRAT RFRLRTHDDG YVVLEAATDS
     LLGNVAEQKL DDDVLRAPIS DTEAWVAAEQ VAPLKRTLYE AGYPVRDDRD LESGEPLAVD
     LHLDLRPYQQ DWVDRFEDAS AGVLVGPPGS GKTVAAMGAL ESVGGETLVL VPSRELATQW
     REELLANTSL TGDQIGEYHG GEKRVRPVTI ATYQTAGMDR HRHVFDDREW GLIVYDEVHH
     IPAEVARRSA SLQSKHRLGL TATPVREDDK EADIYTLVGR PIGTDWDALF DAGFVAEPEV
     EIRYVPWRDD DDRYEYAAAS GHTRRRLAAE NPAKEAEIEH LVDQHGDTQA LVFVDYLSQG
     ERIAERLDAP FVNGETPHSR RETHFDQFRT GALDALVVSR IGDEGIDLPD AEFAVVASGL
     GGSRRQGAQR AGRTMRPGSQ SLLFVLATRG TEEEDHARSR MRHLSTKGVR VTESDASHSP