ID   RAD28_YEAST             Reviewed;         506 AA.
AC   Q12021; D6VS14;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 158.
DE   RecName: Full=Radiation-sensitive protein 28;
GN   Name=RAD28; OrderedLocusNames=YDR030C; ORFNames=YD9813.08c;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   FUNCTION.
RX   PubMed=8830695; DOI=10.1128/jb.178.20.5977-5988.1996;
RA   Bhatia P.K., Verhage R.A., Brouwer J., Friedberg E.C.;
RT   "Molecular cloning and characterization of Saccharomyces cerevisiae RAD28,
RT   the yeast homolog of the human Cockayne syndrome A (CSA) gene.";
RL   J. Bacteriol. 178:5977-5988(1996).
RN   [4]
RP   FUNCTION.
RX   PubMed=9336455; DOI=10.1093/nar/25.21.4257;
RA   Reagan M.S., Friedberg E.C.;
RT   "Recovery of RNA polymerase II synthesis following DNA damage in mutants of
RT   Saccharomyces cerevisiae defective in nucleotide excision repair.";
RL   Nucleic Acids Res. 25:4257-4263(1997).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA   Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-terminal
RT   acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC   -!- FUNCTION: Involved in transcription-coupled repair nucleotide excision
CC       repair (NER) of UV-induced DNA lesions. {ECO:0000269|PubMed:8830695,
CC       ECO:0000269|PubMed:9336455}.
CC   -!- INTERACTION:
CC       Q12021; P39009: DUN1; NbExp=2; IntAct=EBI-29718, EBI-6194;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z47814; CAA87809.1; -; Genomic_DNA.
DR   EMBL; Z74326; CAA98852.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA11874.1; -; Genomic_DNA.
DR   PIR; S50937; S50937.
DR   RefSeq; NP_010313.3; NM_001180338.3.
DR   AlphaFoldDB; Q12021; -.
DR   BioGRID; 32079; 35.
DR   DIP; DIP-5177N; -.
DR   IntAct; Q12021; 13.
DR   STRING; 4932.YDR030C; -.
DR   PaxDb; Q12021; -.
DR   PRIDE; Q12021; -.
DR   EnsemblFungi; YDR030C_mRNA; YDR030C; YDR030C.
DR   GeneID; 851594; -.
DR   KEGG; sce:YDR030C; -.
DR   SGD; S000002437; RAD28.
DR   VEuPathDB; FungiDB:YDR030C; -.
DR   eggNOG; KOG4155; Eukaryota.
DR   GeneTree; ENSGT00940000176625; -.
DR   HOGENOM; CLU_042821_0_0_1; -.
DR   InParanoid; Q12021; -.
DR   OMA; DNGLFFT; -.
DR   BioCyc; YEAST:G3O-29645-MON; -.
DR   Reactome; R-SCE-6781823; Formation of TC-NER Pre-Incision Complex.
DR   Reactome; R-SCE-6782135; Dual incision in TC-NER.
DR   Reactome; R-SCE-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR   PRO; PR:Q12021; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; Q12021; protein.
DR   GO; GO:0031464; C:Cul4A-RING E3 ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:0000109; C:nucleotide-excision repair complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IC:SGD.
DR   GO; GO:0006281; P:DNA repair; IGI:SGD.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR   GO; GO:0006283; P:transcription-coupled nucleotide-excision repair; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR042238; Rad28/ERCC8/Ckn1/ATCSA-1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR46202; PTHR46202; 1.
DR   Pfam; PF00400; WD40; 2.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 4.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   DNA damage; DNA repair; Nucleus; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..506
FT                   /note="Radiation-sensitive protein 28"
FT                   /id="PRO_0000268697"
FT   REPEAT          55..94
FT                   /note="WD 1"
FT   REPEAT          193..233
FT                   /note="WD 2"
FT   REPEAT          285..325
FT                   /note="WD 3"
FT   REPEAT          357..396
FT                   /note="WD 4"
FT   REPEAT          404..451
FT                   /note="WD 5"
SQ   SEQUENCE   506 AA;  58230 MW;  3517F97D9C1F4874 CRC64;
     MDPFLEFRVG NISLNEFYRR TIQSEFERIL EDPLSNMKNY RFSKQSNYST KEKTPLSIGV
     NCLDIDDTGQ VLLGGGDDGS LSIWGLDESL HRNDEGEQEL INKRLNYIKR QPHQSDDEPA
     QIMGYKNKRT RINDNNTMRL VHSFQTQRNK YRMYRQSSAA VPVQRSHISN KTDSPIGFSE
     TLSETDSEAS ISHHKYGITT LKWYKADNGM FFTGSNDKTV KIWDTNRFEA VQDINLGYKI
     NQIDNNVVDD SSLLVVASED YYPRLIDLRT MNSGVTALGM GNQTRMQSEI LCCKFNPVRE
     QIIACGDMEG GVKLWDLRMR NRLYSELKRN KNRFKTINND DNDDQSDVYF SSNQSKAHLR
     CCSDIVWNSE GSELCSVGMD GKLNVWRPFT EILQPEGLAS YSQLGTQDLS RIKYKKRVSR
     RLLWFDKFLL CITDNGEVEI YNTEEKKLWN KLEYPMVNQV KKNQASHCQF SSMIVQTNIM
     NSVGLKLFFG TNNNTVSDGG SIFECS