RAD33_YEAST
ID RAD33_YEAST Reviewed; 177 AA.
AC Q04231; D6VZG3;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=DNA repair protein RAD33;
GN Name=RAD33; OrderedLocusNames=YML011C; ORFNames=YM9571.07C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION.
RX PubMed=16595192; DOI=10.1016/j.dnarep.2006.02.002;
RA den Dulk B., Sun S.M., de Ruijter M., Brandsma J.A., Brouwer J.;
RT "Rad33, a new factor involved in nucleotide excision repair in
RT Saccharomyces cerevisiae.";
RL DNA Repair 5:683-692(2006).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [7]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-19, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- FUNCTION: Involved in nucleotide excision repair (NER) of damaged DNA.
CC Required for the repair of RNA polymerase I-transcribed rDNA and RNA
CC polymerase II-transcribed DNA regions. May have a role in stabilizing
CC the DNA repair proteins RAD4 and RAD34. {ECO:0000269|PubMed:16595192}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1080 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z49810; CAA89941.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09887.1; -; Genomic_DNA.
DR PIR; S55108; S55108.
DR RefSeq; NP_013702.1; NM_001182368.1.
DR PDB; 7K04; EM; 9.25 A; E=1-177.
DR PDB; 7M2U; EM; 8.20 A; E=1-177.
DR PDBsum; 7K04; -.
DR PDBsum; 7M2U; -.
DR AlphaFoldDB; Q04231; -.
DR SMR; Q04231; -.
DR BioGRID; 35158; 45.
DR DIP; DIP-2124N; -.
DR IntAct; Q04231; 3.
DR MINT; Q04231; -.
DR STRING; 4932.YML011C; -.
DR iPTMnet; Q04231; -.
DR MaxQB; Q04231; -.
DR PaxDb; Q04231; -.
DR PRIDE; Q04231; -.
DR EnsemblFungi; YML011C_mRNA; YML011C; YML011C.
DR GeneID; 854998; -.
DR KEGG; sce:YML011C; -.
DR SGD; S000004472; RAD33.
DR VEuPathDB; FungiDB:YML011C; -.
DR eggNOG; ENOG502RZDT; Eukaryota.
DR HOGENOM; CLU_117800_1_0_1; -.
DR InParanoid; Q04231; -.
DR OMA; EMISCAT; -.
DR BioCyc; YEAST:G3O-32615-MON; -.
DR PRO; PR:Q04231; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04231; protein.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0006289; P:nucleotide-excision repair; IMP:SGD.
DR InterPro; IPR014841; Rad33.
DR Pfam; PF08730; Rad33; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; DNA damage; DNA repair; Isopeptide bond;
KW Nucleus; Reference proteome; Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..177
FT /note="DNA repair protein RAD33"
FT /id="PRO_0000203263"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CROSSLNK 19
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
SQ SEQUENCE 177 AA; 20270 MW; 23BAE68F510EED31 CRC64;
MSKSTNVSYE RVELFENPKV PIEVEDEILE KYAESSLDHD MTVNELPRFF KDLQLEPTIW
KLVRNEDVII EGTDVIDFTK LVRCTCQLLI LMNNLTVIDD LWSMLIRNCG RDVDFPQVAL
RDHVLSVKDL QKISNLIGAD QSSGTIEMIS CATDGKRLFM TYLDFGCVLG KLGYLKM
